Nuclear antigens in normal and leukaemic leucocytes: A histochemical study using human auto‐immune antinuclear antibodies

Beck, J. Swanson; Paterson, Joan C.

doi:10.1002/path.1700900225

J. Pathol.

1965

DOI: 10.1002/path.1700900225

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Nuclear antigens in normal and leukaemic leucocytes: A histochemical study using human auto‐immune antinuclear antibodies

J. Swanson Beck

Joan C. Paterson

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1967

1978

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Cited by 6 publications

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References 25 publications

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“…These antibodies react not only with the patients' own nuclei, but also with nuclei from other individuals and from a wide range of animal species (1,3,4,6); thus these antinuclear antibodies are immunochemically specific but not species specific. Human antinuclear antibodies can be used as discriminating cytochemical tools with the immunofluorescence technic ( 5 , 8 ) and with immuno-electron microscopy ( 10). They have also been used recently to investigate the antigenicity…”

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confidence: 99%

Distribution of the DNA and the DNA‐Histone Antigens in the Nuclei of Free‐Living and Parasitic Sarcomastigophora

Stewart

Beck

1967

The Journal of Protozoology

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SYNOPSIS. Sixty‐seven species of the subphylum Sarcomastigophora were stained with 2 human antinuclear antibodies to detect the corresponding nuclear antigens, using the indirect immunofluorescence technic. One antinuclear serum contained an antibody to DNA and the other an antibody to DNA linked to histone (DNA‐H). Results were classified according to the revised classification of the Society of Protozoologists' Committee on Taxonomy and Taxanomic Problems (1964). The DNA antigen was detected in the nuclei of all species studied. The DNA‐H antigen was found in all the freeliving species examined, but it was not present in any of the 40 parasitic species tested. The free‐living species of Sarcodina studied represented the orders Amoebida (families Amoebidae, Mayorellidae) and Actinophryida. Parasitic species tested were all from the Amoebida. Within the Mastigophora, free‐living species were examined from the orders Cryptomonadida, Euglenida, Volvocida, Dinoflagellida, Kinetoplastida (suborder Bodonina), and Rhizomastigida. Parasitic species studied represented the orders Kinetoplastida (suborder Trypanosomatina), Trichomonadida and Rhizomastigida. It is suggested that the absence of the DNA‐H antigen in parasitic species is perhaps linked with the inability of these species to survive in a free‐living environment.

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confidence: 99%

Distribution of the DNA and the DNA‐Histone Antigens in the Nuclei of Free‐Living and Parasitic Sarcomastigophora

Stewart

Beck

1967

The Journal of Protozoology

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In Vivo and In Vitro Anti‐Nuclear Reactions in Lupus Patients

Zweiman

Hildreth

1968

Arthritis & Rheumatism

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HE antinuclear factors found in the T sera of patients with systemic lupus erythematosus (SLE) bind to the nuclei of a variety of mammalian cells as measured by immunofluorescent techniques.l Yet tissues from lupus patients rarely show gamma globulin (presumably antinuclear factors) bound in vivo to cell nuclei.2J Evidence has been presented4 suggesting that tissue nuclei of lupus patients do not react with antinuclear factors in vitro as consistently as do nuclei from normal individuals -possibly implying a lack of available binding sites in the tissues of lupus patients.In this study, the capacity of leukocyte nuclei from lupus patients to react in vitro and in oivo with various types of antinuclear factors was studied, with a comparison of the patterns of nuclear fluorescence induced by certain antinuclear MATERIAL AND METHODSSmears were made from the peripheral blood of normal subjects and of 18 patients with typical SLE (with varying degrees of disease activity). Serum specimens were obtained simultaneously from these individuals. These sera exhibited a variety of nuclear fluorescence patterns (diffuse, peripheral rim, speckled) when incubated with normal leukocyte nuclei in o h . In addition, 2 other serum specimens from lupus patients (patients RS and HM) were used. One serum (RS) had consistently induced "peripheral rim" nuclear fluorescence, and the other serum (HM) had led to a combination of "speckled" and "diffuse" nuclear fluorescence when incubated with leukocytes of normal subjects.Peripheral blood smears were utilized in the direct or indirect fluorescent antibody method of detccting antinuclear factors as advocated by Barnett et al.7 Smears were fixed in 95 per cent ethanol at 37 C for 30 minutes and then dried prior to use, evcept where noted below. RESULTSA peripheral blood smear from each lupus patient was incubated with fluorescein-labelled anti-human gamma globulin goat serum (Hyland Labs) looking for gamma globulin bound in viuo to cell nuclei. In 17 of the 18 smears, no nuclear fluorescence was seen. In one smear, weak nuclear fluorescence was seen in a minority of the leukocytes (Table 1).A serum specimen from each lupus p7-tient was incubated for 30 minutes with an autologous peripheral blood smear and with a normal donor's blood smear. After being washed with phosphate-buffered saine (PBS), the smears were then incubated with fluorescein-labelled anti-human gamma globulin goat serum. Nuclear fluorescence was seen after incubation with 17 of the 18 sera whether autologous or normal donor blood smears were used (Table 1). The nuclear fluorescence pattern seen after incubation of a particular lupus serum with normal blood leukocytes was generally but not always similar to the pattern resulting

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Clinical features of the arthritis of mixed connective tissue disease

Halla

Hardin

1978

Arthritis & Rheumatism

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Seventeen of 19 patients with mixed connective tissue disease (MCTD) had arthritis as a significant initial feature of their disease; 8 were given an initial diagnosis of rheumatoid arthritis (RA) and 4 received chrysotherapy. RA‐like hand deformities were present in 35% and contractures and/or persistent loss of joint motion in 47%. Joint radiographs showed abnormalities in 41% and included erosions and/or cysts in 30%. The arthritis of MCTD may be both erosive and deforming and this disease should be considered in patients presenting as RA with unusual features.

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Nuclear antigens in normal and leukaemic leucocytes: A histochemical study using human auto‐immune antinuclear antibodies

Cited by 6 publications

References 25 publications

Distribution of the DNA and the DNA‐Histone Antigens in the Nuclei of Free‐Living and Parasitic Sarcomastigophora

Distribution of the DNA and the DNA‐Histone Antigens in the Nuclei of Free‐Living and Parasitic Sarcomastigophora

In Vivo and In Vitro Anti‐Nuclear Reactions in Lupus Patients

Clinical features of the arthritis of mixed connective tissue disease

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