Nuclear Androgen Receptors in the Mouse Kidney: Validation of a New Assay*

Isomaa, Veli; Pajunen, Antti; Bardin, C. Wayne; Jänne, Olli A.

doi:10.1210/endo-111-3-833

Cited by 119 publications

(86 citation statements)

References 38 publications

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“…Although mRNA induction accounts fully for the induction of OrnDCase in the experimental system considered here, a different result was found when the mechanism of induction of the enzyme in mouse kidney by androgen was examined (31,41,42). In that system, an increase in enzyme half-life augmented the inductive effect of increased OrnDCase mRNA levels.…”

Section: Discussionmentioning

confidence: 64%

Nerve growth factor rapidly induces ornithine decarboxylase mRNA in PC12 rat pheochromocytoma cells.

Feinstein¹,

Dana²,

McConlogue³

et al. 1985

Proc. Natl. Acad. Sci. U.S.A.

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The mechanism by which nerve growth factor (NGF) stimulates ornithine decarboxylase (OrnDCase; EC 4.1.1.17) activity in the rat pheochromocytoma cell line PC12 was investigated. As demonstrated previously, NGF rapidly induces OrnDCase activity in a dose-dependent manner, with maximal enzymatic activity at 4-6 hr after exposure to NGF. Activity subsequently returns to near basal levels. A cloned OrnDCase cDNA was used to analyze the levels of OrnDCase RNA. In response to NGF administration, OrnDCase RNA levels were induced. The time course of the OrnDCase RNA induction paralleled that of the enzyme activity induction, and the magnitude of both inductions was quantitatively the same. Increased concentration of OrnDCase RNA was clearly detected at the earliest time point examined, 2 hr. No change was observed in the size of OrnDCase RNA. The dose-response curves for both RNA and enzyme activity inductions were also similar. Thus, increased OrnDCase RNA levels fully account for, and are responsible for, the induction of activity. Further, one-third of the OrnDCase RNA induction was unaffected by cycloheximide treatment but was fully blocked by actinomycin D treatment, suggesting that NGF acts through at least two mechanisms to mediate the OrnDCase induction. The first mechanism is cycloheximide insensitive and the second is mediated through an event requiring ongoing protein synthesis. Both mechanisms require ongoing transcription, as evidenced by the complete sensitivity of the induction process to actinomycin D.

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Section: Discussionmentioning

confidence: 64%

Nerve growth factor rapidly induces ornithine decarboxylase mRNA in PC12 rat pheochromocytoma cells.

Feinstein¹,

Dana²,

McConlogue³

et al. 1985

Proc. Natl. Acad. Sci. U.S.A.

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“…Stabilization of ODC is involved in various types of ODC induction, such as that in the kidney of androgen-treated female mice [37][38][39] and that in cells treated with amino acids such as asparagine or glutamine [40][41][42], hypotonic medium [41,[43][44][45], or inhibitors of polyamine synthesis [46][47][48][49][50]. Conversely, ODC is rapidly destabilized by removal of stabilizing amino acids [40], reversal to isotonicity [40,45], or addition of polyamines [51][52][53].…”

Section: Historical Background Rapid Turnover Of Odcmentioning

confidence: 99%

Rapid and regulated degradation of ornithine decarboxylase

Hayashi

Murakami

1995

Biochemical Journal

194

131

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“…However, the preparation of monospecific anti-ODC antibodies (Kameji et al, 1982;Persson, 1982;Isomaa et al, 1983;Seely & Pegg, 1983) as well as ODC cDNAs (Berger et al, 1984;Kahana & Nathans, 1984;Kontula et al, 1984;McConlogue et al, 1984) has provided adequate tools for such studies. In previous reports we have demonstrated that the polyamines exert a feedback control of ODC synthesis (Persson et al, 1985(Persson et al, , 1986.…”

Section: Introductionmentioning

confidence: 99%

Feedback control of ornithine decarboxylase expression by polyamines. Analysis of ornithine decarboxylase mRNA distribution in polysome profiles and of translation of this mRNA in vitro

Holm

Persson

Stjernborg

et al. 1989

Biochemical Journal

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Cell growth and differentiation require the presence of optimal concentrations of polyamines. Ornithine decarboxylase (ODC) catalyses the first and rate-controlling step in polyamine synthesis. In studies using cultures of Ehrlich ascites-tumour cells, we have shown that the expression of ODC is subject to feedback regulation by the polyamines. A decrease in the cellular polyamine concentration results in a compensatory increase in the synthesis of ODC, whereas an increase in polyamine concentration results in suppression of ODC synthesis. These changes in ODC synthesis were attributed to changes in the efficiency of ODC mRNA translation, because the steady-state amount of ODC mRNA remained constant. We now show that the number of ribosomes associated with ODC mRNA is low, and that the increase in ODC mRNA translation takes place without a shift in the distribution of ODC mRNA towards larger polysomes. This finding indicates that the polyamines regulate the efficiency of ODC mRNA translation by co-ordinately affecting the rates of initiation and elongation. By analysing ODC mRNA translation in vitro, using a rabbit reticulocyte lysate, polyadenylated RNA from a cell line with an amplified ODC gene, and a monospecific anti-ODC antibody, we also show that spermidine, but not putrescine, exerts a direct regulatory effect on ODC synthesis.

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Nuclear Androgen Receptors in the Mouse Kidney: Validation of a New Assay*

Cited by 119 publications

References 38 publications

Nerve growth factor rapidly induces ornithine decarboxylase mRNA in PC12 rat pheochromocytoma cells.

Nerve growth factor rapidly induces ornithine decarboxylase mRNA in PC12 rat pheochromocytoma cells.

Rapid and regulated degradation of ornithine decarboxylase

Feedback control of ornithine decarboxylase expression by polyamines. Analysis of ornithine decarboxylase mRNA distribution in polysome profiles and of translation of this mRNA in vitro

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