Nuclear and mitochondrial genetic markers highly conserved between Chinese and Philippine Schistosoma japonicum

Bowles, Josephine; Hope, Michelle; Tiu, Wilfred U.; Liu, Xushian; McManus, Donald P.

doi:10.1016/0001-706x(93)90079-q

Cited by 213 publications

(125 citation statements)

References 28 publications

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“…cox1 Sequences have been widely used to define species limits and phylogenetic affinities in the platyhelminthes (Bowles et al 1993(Bowles et al , 1995Bowles and McManus 1994;Iwagami et al 2000Iwagami et al , 2003Morgan et al 2003;Razo-Mendivil et al 2004; nevertheless, most of those studies used a region of the cox1 that overlaps only in a short fragment at the 5 0 end of the standard barcode. The generation of primers that amplify the standard barcode region for a wide variety of platyhelminth groups has been a challenge (Moszczynska et al 2009), and is still at the experimental stage.…”

Section: Discussionmentioning

confidence: 99%

“…Amplification and sequencing were performed using the primers JB3 5 0 -TTTTT-TGGG CATCCTGAGGTTTAT-3 0 (forward) and JB4.5 5 0 -TAAAGAAAGAACATAATG AAAATG-3 0 (reverse) (Bowles et al 1993 Note: Minimum and maximum values are shown for groups with multiple sequences and intraspecific variation. O, Oaxaca; V, Veracruz; C, Colima; P, Puebla; and JO, Jalisco-Oaxaca.…”

Section: Laboratory Methodsmentioning

confidence: 99%

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Evidence of new species ofHaematoloechus(Platyhelminthes: Digenea) using partialcox1sequences

León‐Régàgnon

2010

Mitochondrial DNA

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To cite this article: Virginia León-Règagnon (2010) Evidence of new species of Haematoloechus (Platyhelminthes: Digenea) using partial cox1 sequences, Mitochondrial DNA, 21:sup1, 12-17, DOI: 10.3109/19401736.2010.523700 To link to this article: https://doi.org/10. 3109/19401736.2010.523700 Abstract Background and aims. Haematoloechus, digeneans parasites of amphibians, is a species-rich genus with more than 50 species around the globe. Establishing an accurate taxonomy for this group has been difficult due to high intraspecific variability. Nuclear DNA sequences have given independent information about species validity and phylogeny of the group.Materials and methods. In this paper, I test the performance of partial sequences of the mitochondrial cytochrome c oxidase subunit I (cox1) gene in the differentiation of recognized species of the genus and in the detection of potential new taxa. Samples from 13 nominal species were sequenced, plus four samples that could not be assigned to any described species based on morphology.Results. Parsimony analysis of the amplified 360 bp fragment resulted in six most parsimonious trees showing the same grouping of samples, differing in the samples' arrangement within those groups. All 13 species were recovered on the trees, and five potential new species are shown.Conclusion. Additional sampling and sequencing is necessary to support this hypothesis, but with this preliminary information the search for diagnostic characters that allow the description of the new taxa is less difficult.

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Section: Discussionmentioning

confidence: 99%

Section: Laboratory Methodsmentioning

confidence: 99%

Evidence of new species ofHaematoloechus(Platyhelminthes: Digenea) using partialcox1sequences

León‐Régàgnon

2010

Mitochondrial DNA

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“…The cytochrome oxidase subunit 1 gene, or COI gene, of each individual was amplified using the JB3 (S'-TTTTTTGGGCATCCTGAGGTTTAT-3') of Bowles et al (1993) as the forward primer. The reverse primer used was trem.coxl.rrnl (S'AATCATGATGCAAAAGGTA-S') of Králová-Hromadová et al (2001).…”

Section: Methodsmentioning

confidence: 99%

Trematode parasites of Otago Harbour (New Zealand) soft‐sediment intertidal ecosystems: Life cycles, ecological roles and DNA barcodes

Leung

Donald

Keeney

et al. 2009

New Zealand Journal of Marine and Freshwater Research

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Parasites, in particular trematodes (Platyhelminthes: Digenea), play major roles in the population dynamics and community structure of invertebrates on soft-sediment mudflats. Here, we provide a list of the 20 trematode species currently known to infect molluscs, crustaceans and polychaetes from Otago Harbour (New Zealand) soft-sediment intertidal areas, as well as information on their transmission modes, life cycles, andknown ecological impacts. Several of the host-parasite species combinations recorded here are reported for the first time. We also provide DNA barcodes, based on sequences of the cytochrome oxidase subunit l (CO1) gene, for 19 of the 20 trematode species, to facilitate future identification of these parasites in marine ecological studies.

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“…For PCR-amplification we used three rDNA marker gene regions: 18S, 28S and internal transcribed spacer 2 (ITS2) and the mitochondrial cytochrome oxidase subunit 1 (CO1) region. Primers used for the respective gene region are detailed as follows: ITS2: 3S (Forward)/ A28 (Reverse) (Bowles et al, 1995) 18S: EukA (Forward)/ EukB (Reverse) (Diez et al, 2001) 28S: dig12 (Forward)/ 1500R (Reverse) (Tkach et al, 2000) mtCO1: JB3 (Forward)/ JB4.5 (Reverse) (Bowles et al, 1993). The thermal gradient of these marker regions started with an initial denaturation at 94 ºC (5min), annealing -for 18S at 58 ºC (1.10 min), 28S at 57 ºC (1 min), ITS2 at 57 ºC (1.10 min) and CO1 at 56 ºC (1.10 min), and final extension at 72 ºC (10 min).…”

Section: Molecular Studymentioning

confidence: 99%

Morphological and molecular characterization of Posthodiplostomum sp. (Digenea: Diplostomidae) metacercaria in the muscles of snakeheads (Channa punctata) from Manipur, India

Athokpam

Tandon

2014

Helminthologia

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141 SummaryThe spotted snakehead, Channa punctata Bloch, 1793, is a locally important fish species commonly consumed by the natives in the state of Manipur, Northeast India. The fish host C. punctata from Lamphel area revealed a diplostomid metacercarial infection. Morphologically, the recovered metacercaria was identified as a species of Posthodiplostomum Dubois, 1936. Molecular characterization using the ribosomal RNA genes (rDNA 18S, ITS2 and 28S regions) and the mitochondrial CO1 region supplements the identification. Molecular analysis revealed the metacercaria to be closely related to Posthodiplostomum sp. Japan isolate, with sequence similarity variation from 97.5 -99.7 % while considering for the three rDNA markers. The secondary structure of the ITS2 region further corroborated these results; the typical four-helix model, when compared to the taxon from Japan, showed differences only in twelve bases (with seven transitions and five transversions). In phylogenetic analysis also, the metacercaria claded with the genus Posthodiplostomum, coming closer to the Japanese isolate, thus supplementing the morphological identification of the metacercaria.

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Nuclear and mitochondrial genetic markers highly conserved between Chinese and Philippine Schistosoma japonicum

Cited by 213 publications

References 28 publications

Evidence of new species ofHaematoloechus(Platyhelminthes: Digenea) using partialcox1sequences

Evidence of new species ofHaematoloechus(Platyhelminthes: Digenea) using partialcox1sequences

Trematode parasites of Otago Harbour (New Zealand) soft‐sediment intertidal ecosystems: Life cycles, ecological roles and DNA barcodes

Morphological and molecular characterization of Posthodiplostomum sp. (Digenea: Diplostomidae) metacercaria in the muscles of snakeheads (Channa punctata) from Manipur, India

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