Nuclear and Cytoplasmic Messenger-like RNA and Their Relation to the Active Messenger RNA in Polyribosomes of HeLa Cells

Scherrer, Klaus; Spohr, Georges; Granboulan, Nicole; Morel, Carlos Médicis; Grosclaude, Jeanne; Chezzi, Carlo

doi:10.1101/sqb.1970.035.01.070

Cited by 74 publications

(29 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Recently a cytoplasmic DNA dependent RNA polymerase (RNA polymerase C) has been described [22] which could be involved in the transcription of the "communication DNA" in the cytoplasm. Furthermore, there is evidence that no precise transcriptional control is executed within the nucleus [5,6].…”

Section: Resultsmentioning

confidence: 99%

Transport of nuclear DNA into the cytoplasm in animal cells The complexity of the transported DNA

Koch¹,

Götz²

1972

FEBS Letters

View full text Add to dashboard Cite

Section: Resultsmentioning

confidence: 99%

Transport of nuclear DNA into the cytoplasm in animal cells The complexity of the transported DNA

Koch¹,

Götz²

1972

FEBS Letters

View full text Add to dashboard Cite

“…Thus, informational (or spacer) sequences will represent the bulk of the hybridized RNA. However, since a large fraction of the nascent RNA is turning over [2] , the specific activity of the A-rich sequences among the hybridized RNA molecules will be higher than that of the informational (or spacer) part, unless the RNA is labelled uniformly.…”

Section: Discussionmentioning

confidence: 99%

“…Hybridization was carried out either in liquid phase and evaluated by the Sephadex technique [3] or on filters by the urea low-temperature technique [2] . In the latter case RNA was isolated from nitrocellulose filters either by heating for 15 min to 90" with 0.01 X SSC or to 60" in the same buffer with 5% formamide.…”

Section: Methodsmentioning

confidence: 99%

“…By measuring actual concentrations of nucleotides we can determine the composition of the hybridized RNA which corresponds to the steady state population of mlRNa, i.e., the pool of metabolized and stabilized molecules in nucleus or cytoplasm [2]. The base composition of the hybrid, identical to that of the steady state mlRNA suggests that RNA corresponding to average structural genes has been hybridized to some extent during the 4 days of incubation.…”

Section: North-holland Publishing Company ~ Amsterdammentioning

confidence: 99%

“…

We interpreted this observation as demonstrating the existence in mlRNA, and particularly in the nascent fraction, of homologous signal or spacer loci, distributed over the genome [2]. Recently the existence of A-rich sequences in vaccinia-virus specific mRNA [4] and in HeLa cell RNA was described [S] and A-rich sequences were found in globin mRNA PI .

…”

mentioning

confidence: 93%

See 2 more Smart Citations

Adenosine‐rich sequences in rapidly hybridizing messenger‐like RNA and their possible significance for reiterated base sequences in eukaryotic DNA

Scherrer¹

1971

FEBS Letters

View full text Add to dashboard Cite

show abstract

RNA‐dependent nuclear matrix contains a 33 kb globin full domain transcript as well as prosomes but no 26S proteasomes

Ioudinkova

Razin

Borunova

et al. 2004

J of Cellular Biochemistry

View full text Add to dashboard Cite

Previously, we have shown that in murine myoblasts prosomes are constituents of the nuclear matrix; a major part of the latter was found to be RNase sensitive. Here, we further define the RNA-dependent matrix in avian erythroblastosis virus (AEV) transformed erythroid cells in relation to its structure, presence of specific RNA, prosomes and/or proteasomes. These cells transcribe but do not express globin genes prior to induction. Electron micrographs show little difference in matrices treated with DNase alone or with both, DNase and RNase. In situ hybridization with alpha globin riboprobes shows that this matrix includes globin transcripts. Of particular interest is that, apparently, a nearly 35 kb long globin full domain transcript (FDT), including genes, intergenic regions and a large upstream domain is a part of the RNA-dependent nuclear matrix. The 23K-type of prosomes, previously shown to be co-localized with globin transcripts in the nuclear RNA processing centers, were found all over the nuclear matrix. Other types of prosomes show different distributions in the intact cell but similar distribution patterns on the matrix. Globin transcripts and at least 80% of prosomes disappear from matrices upon RNase treatment. Interestingly, the 19S proteasome modulator complex is insensitive to RNase treatment. Only 20S prosomes but not 26S proteasomes are thus part of the RNA-dependent nuclear matrix. We suggest that giant pre-mRNA and FDTs in processing, aligning prosomes and other RNA-binding proteins are involved in the organization of the dynamic nuclear matrix. It is proposed that the putative function of RNA within the nuclear matrix and, thus, the nuclear dynamic architecture, might explain the giant size and complex organization of primary transcripts and their introns.

show abstract

Nuclear and Cytoplasmic Messenger-like RNA and Their Relation to the Active Messenger RNA in Polyribosomes of HeLa Cells

Cited by 74 publications

References 0 publications

Transport of nuclear DNA into the cytoplasm in animal cells The complexity of the transported DNA

Transport of nuclear DNA into the cytoplasm in animal cells The complexity of the transported DNA

Adenosine‐rich sequences in rapidly hybridizing messenger‐like RNA and their possible significance for reiterated base sequences in eukaryotic DNA

RNA‐dependent nuclear matrix contains a 33 kb globin full domain transcript as well as prosomes but no 26S proteasomes

Contact Info

Product

Resources

About