Nuclear abnormalities in cells from nasal epithelium: a promising assay to evaluate DNA damage related to air pollution in infants

Mergener, Michelle; Rhoden, Cláudia Ramos; Amantéa, Sérgio L.

doi:10.1016/j.jpedp.2014.04.008

Cited by 5 publications

(6 citation statements)

References 23 publications

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“…2). (11) Claudia et al found that nuclear abnormalities were more in formalin-exposed individuals as stated in our study, (12) relative increase in the nuclear abnormalities found in the outdoor formaldehyde exposure studies were well in correlation with our study which included the indoor formalin-exposed individuals. (13) Metanuclear abnormalities were more in the workers and faculties working in the dissection hall for more than 10 years of exposure.…”

Section: Discussionsupporting

confidence: 92%

Metanuclear Abnormalities in Formalin-Exposed Individuals

Natarajan¹,

Sundarapandian²,

Sakthivel³

2017

jemds

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Section: Discussionsupporting

confidence: 92%

Metanuclear Abnormalities in Formalin-Exposed Individuals

Natarajan¹,

Sundarapandian²,

Sakthivel³

2017

jemds

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“…The results obtained from the umbilical cord blood of newborns from Ahome are very similar to the rates of nuclear abnormalities found in other studies of neonates and children 37 . These studies found lower frequencies (0.13%) of cells with micronuclei, 1.20% karyorrhexis, 0.03% pyknosis, 10.85% karyolysis, 1.11% condensed chromatin, 0.54 binucleated cells and 0.02% nuclear buds.…”

Section: Discussionsupporting

confidence: 87%

“…Mergener et al 37 . through a literature survey concluded that the frequency of nuclear abnormalities is very low at birth.…”

Section: Discussionmentioning

confidence: 98%

Nuclear abnormalities in umbilical cord blood lymphocytes of newborns from the Ahome and Guasave municipalities in Sinaloa, Mexico

Martínez-Valenzuela

Huichapan

Martínez

et al. 2020

J of Obstet and Gynaecol

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Aim We measured the frequency of nuclear abnormalities of 210 blood samples from the umbilical cord, since human fetuses are exposed to environmental mixtures of pesticides that induce DNA damage. Methods The determinations were made through the micronucleus assay test in lymphocytes from the umbilical cord blood of newborns whose mothers live in Ahome (n = 105) and Guasave (n = 105), Sinaloa, Mexico. Results The average frequency of anomalies in 1000 cells were, respectively: micronucleus 0.4 vs. 2.9, pyknotic cells 18.3 vs. 109.2, chromatin condensation 7.7 vs. 150.1, karyolitic cells 1.8 vs. 24.4, and binucleated cells 4.9 vs. 74.6. The calculated Pearson correlation factors of nuclear abnormality frequencies between both municipalities were low and negative, suggesting that they did not correlate between the Ahome and Guasave newborns and indicating a higher number of mothers exposed in Guasave. Conclusion Our data suggest that monitoring nuclear abnormalities in umbilical cord blood samples could be a useful tool to identify transplacental mutagens perfusion that is being discharged into the local environment.

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“…3). Total number of binucleated cells found manually by Ramadhan D was 64 as seen in our study in the 6-10 and 10 -15 years exposure group [13] In our study the nuclear abnormalities were more in formalin-exposed individuals as stated by Mergener et al, [14] relative increase in the nuclear abnormalities found in formaldehyde exposure studies were well in correlation with our study [15] Metanuclear abnormalities were more in the workers working in the dissection hall for more than 10 years of exposure. These results were well in correlation with a study conducted by Shekawat S et al [16] Leon Cleres Penido et al stated that students with a more extensive workload in anatomy laboratory showed increase in karyorrhexis, the same results were seen in our study and hence we need to reduce the duration of formalin exposure in the dissection hall and should advise the students to wear mask to prevent the absorption of formalin in the nasal epithelium [17].…”

Section: Resultssupporting

confidence: 69%

Formalin Induced Nuclear Abnormalities in Relation With Duration of Exposure: A Study of Formalin Exposed Anatomists, Attenders and Students

Natarajan¹,

Subramanian²,

Elayaperumal³

2019

IJAR

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Background: Exposure of Genotoxic agents like formalin causes cancer. The duration of exposure and the quantity of exposure plays an important role in inducing the nuclear abnormalities. The nuclear abnormalities are BN, KR, KL.Objective: This study provides an easy and inexpensive way to assess the formalin induced nuclear abnormalities in different groups with different duration of exposure.Materials and methods: Fifty individuals with different duration of exposure were included in our descriptive study. Oral squamous cells were collected with the wooden spatula. The slides were fixed with methanol glutaraldehyde fixative and stained with Giemsa, May-Grunwald stains. After air drying, 1000 cells were screened for nuclear abnormalities like BN, KR, KL.Results: Nuclear abnormalities like KR, KL, BN were assessed. KL is more in less than 1-year exposure group.KR is more in long term exposure groups. BN cells are seen 6 -10 and 10 -15 years exposure group. Conclusion:The genotoxic effect of formalin was confirmed by the increase in the nuclear abnormalities especially by the increase in the frequency of KR in tong term exposed group and KL in less than 1-year exposed group.

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Nuclear abnormalities in cells from nasal epithelium: a promising assay to evaluate DNA damage related to air pollution in infants

Cited by 5 publications

References 23 publications

Metanuclear Abnormalities in Formalin-Exposed Individuals

Metanuclear Abnormalities in Formalin-Exposed Individuals

Nuclear abnormalities in umbilical cord blood lymphocytes of newborns from the Ahome and Guasave municipalities in Sinaloa, Mexico

Formalin Induced Nuclear Abnormalities in Relation With Duration of Exposure: A Study of Formalin Exposed Anatomists, Attenders and Students

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