Nuclear abnormalities in cells from nasal epithelium: a promising assay to evaluate DNA damage related to air pollution in infants

Mergener, Michelle; Rhoden, Cláudia Ramos; Amantéa, Sérgio L.

doi:10.1016/j.jped.2014.04.009

Cited by 6 publications

(4 citation statements)

References 25 publications

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“…Conversely, this study design did not allow to compare the MN and comet test results at high and low air pollutants levels. Various studies found a positive association between air pollution and DNA damage tests at lower levels of air pollutants than that found in our study [40][41][42][43][44][45][46][47][48][49]. However, other studies provided negative or inconsistent results regarding the association between MN frequency in children's cells and air pollutant exposure, highlighting the role of other behavioural factors in the MN occurrence [47,52,53].…”

Section: Discussionsupporting

confidence: 47%

“…A previous study conducted on preschool children (3-6 years old) living in the same town [39] during two consecutive winters, also found a high mean MN frequency in children's buccal mucosa cells, similarly to that found in present study. Other studies evaluated the frequency of MN in exfoliated buccal cells in children [40][41][42][43][44][45][46][47][48][49] and found a significant association between MN frequencies and various air pollutants in children exposed to air pollution.…”

Section: Discussionmentioning

confidence: 99%

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Winter Air Pollution and Genotoxic Effects in Children Living in a Highly Polluted Urban Area

et al. 2021

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Air pollutants, especially PM, have been found to determine various effects on human health, including genotoxic effects. The aim of this study was to assess DNA damage with micronuclei (MN) and comet tests on buccal cells of 6–8 years old children living in an area with high air pollution. Both tests were repeated in the same children in two consecutive winters to compare the levels of DNA damage under different pollution conditions. A complete data set including lifestyle, air pollutants levels and biological sampling was available for 180 children in the two winters. A high mean MN frequency was found in both seasons, with higher value in the first (0.51 ± 0.59) than the second winter (0.40 ± 0.52), whereas DNA damage measured with comet test showed higher damage in the second versus the first winter (visual score 208.8 ± 67.1 vs. 173.2 ± 50.8). The associations between air pollutant levels (CO, NO2, SO2, benzene, O3, PM10, and PM2.5; PM0.5 and PAHs) and DNA damage were investigated at different lag times, and mainly, no significant association was found. This study on repeated measure of MN frequency and DNA damage in children’s buccal did not show an association with various air pollutants evaluated in an area with high levels of air pollution.

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Section: Discussionsupporting

confidence: 47%

Section: Discussionmentioning

confidence: 99%

Winter Air Pollution and Genotoxic Effects in Children Living in a Highly Polluted Urban Area

et al. 2021

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“…The danger of these radiation types primarily originates from their high penetration and ionization energy, which can destroy normal human cells and lead to gene mutation. Long-term exposure to nuclear radiation causes immune decline, cancer, and even immediate death, among other problems, in humans [ 8 , 9 , 10 , 11 , 12 ]. Thus, the effective radiation shielding of nuclear facilities is very important.…”

Section: Introductionmentioning

confidence: 99%

Preparation and Comprehensive Properties of a High-Radiation-Shielding UHPC by Using Magnetite Fine Aggregate

Han

et al. 2022

Materials

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Nuclear technology benefits humans, but it also produces nuclear radiation that harms human health and the environment. Based on the modified Andreasen and Andersen particle packing model for achieving a densely compacted cementitious matrix, a new magnetite ultra-high-performance concrete (MUHPC) was designed using magnetite fine aggregate as a substitute for river sands with 0%, 20%, 40%, 60%, 80%, and 100% replacement ratios. The comprehensive properties of the developed MUHPC were tested and evaluated. These properties were fluidity, static and dynamic compressive strengths, high-temperature performance, antiradiation behaviors, hydration products, and micropore structures. Experimental results indicate that the developed MUHPC has high work performance and static and dynamic mechanical properties. The gamma ray shielding performance of MUHPC substantially improves with increased magnetite fine aggregate. Corresponding with 100% magnetite fine aggregate substitution, the linear attenuation coefficient of MUHPC is enhanced by 56.8% compared with that of ordinary concrete. Magnetite addition does not change the type of cement hydration products but improves the micropore structures of MUHPC and effectively reduces its total porosity and average pore diameter, thereby contributing to its mechanical and radiation shielding properties. The compressive strength and linear attenuation coefficient of the MUHPC can reach 150 MPa and 0.2 cm−1, respectively. In addition, the MUHPC also exhibits superior mechanical and radiation shielding performance at elevated temperatures (<400 °C). Finally, high strength and antiradiation performance support the use of MUHPC in radiation protection materials in the future.

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“…In some respiratory disorders, such as asthma and acute viral bronchiolitis, 7 Micronuclei (MN) have been identified as useful markers of cell damage. MN are capable of signaling events associated with cellular and molecular genotoxicity, providing support for a better understanding of etiology and pathogenesis, and consequently for the establishment of prevention strategies.…”

Section: Introductionmentioning

confidence: 99%

Micronucleus count in nasal epithelial cells from patients with chronic rhinosinusitis and polyps

Drummond

Rhoden

Neto

et al. 2020

Brazilian Journal of Otorhinolaryngology

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Nuclear abnormalities in cells from nasal epithelium: a promising assay to evaluate DNA damage related to air pollution in infants

Abstract: The assay proposed here is suitable for assessing the frequency of nuclear abnormalities from nasal cells in infants.

Cited by 6 publications

References 25 publications

Winter Air Pollution and Genotoxic Effects in Children Living in a Highly Polluted Urban Area

Winter Air Pollution and Genotoxic Effects in Children Living in a Highly Polluted Urban Area

Preparation and Comprehensive Properties of a High-Radiation-Shielding UHPC by Using Magnetite Fine Aggregate

Micronucleus count in nasal epithelial cells from patients with chronic rhinosinusitis and polyps

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