NUCKS overexpression in breast cancer

Drosos, Yiannis; Kouloukoussa, Mirsini; Østvold, Anne Carine; Grundt, Kirsten; Goutas, Nikos; Vlachodimitropoulos, Dimitrios; Havaki, Sophia; Κollia, Panagoula; Kittas, Christos; Marinos, Evangelos

doi:10.1186/1475-2867-9-19

Cited by 41 publications

(39 citation statements)

References 33 publications

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“…There are several scientific reports utilizing array-based Comparative Genomic Hybridization (CGH) techniques, which show that there is a high frequency (59-74%) of gain of chromosome region 1q.32 in breast cancer and this gain is an early event in the cancer progression (Nyante et al, 2004). In harmony with our results Drosos et al (2009) reported in his study that the quantitative, real-time RT-PCR analysis of primary culture cells derived from benign lesions and breast carcinomas revealed higher NUCKS-1 gene expression compared to normal and fibroadenoma culture cells.…”

Section: 837 Effect Of Nucks-1 Overexpression On Cytokines Profilingsupporting

confidence: 78%

Effect of NUCKS-1 Overexpression on Cytokine Profiling in Obese Women with Breast Cancer

Soliman

Zineldeen²,

El-Khadrawy³

2014

Asian Pacific Journal of Cancer Prevention

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Background: Overweight and obesity are recognized as major drivers of cancers including breast cancer. Several cytokines, including interleukin-6 (IL-6), IL-10 and lipocalin 2 (LCN2), as well as dysregulated cell cycle proteins are implicated in breast carcinogenesis. The nuclear, casein kinase and cyclin-dependent kinase substrate-1 (NUCKS-1), is a nuclear DNA-binding protein that has been implicated in several human cancers, including breast cancer. Objectives: The present study was conducted to evaluate NUCKS-1 mRNA expression in breast tissue from obese patients with and without breast cancer and lean controls. NUCKS-1 expression was correlated to cytokine profiles as prognostic and monitoring tools for breast cancer, providing a molecular basis for a causal link between obesity and risk. Materials and Methods: This study included 39 females with breast cancer (G III) that was furtherly subdivided into two subgroups according to cancer grading (G IIIa and G IIIb) and 10 control obese females (G II) in addition to 10 age-matched healthy lean controls (G I). NUCKS-1 expression was studied in breast tissue biopsies by means of real-time PCR (RT-PCR). Serum cytokine profiles were determined by immunoassay. Lipid profiles and glycemic status as well as anthropometric measures werealso recorded for all participants. Results: IL-6, IL-12 and LCN2 were significantly higher in control obese and breast cancer group than their relevant lean controls (p<0.05), while NUCKS-1 mRNA expression was significantly higher in the breast cancer group compared to the other groups (p<0.05). Significant higher levels of IL-6, IL-12, and LCN2 as well as NUCKS-1 mRNA levels were reported in G IIIb than G IIIa, and positively correlated with obesity markers in all obese patients. Conclusions: Evaluation of cytokine levels as well as related gene expression may provide a new tool for understanding interactions for three axes of carcinogenesis, innate immunity, inflammation and cell cycling, and hope for new strategies of management.

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Section: 837 Effect Of Nucks-1 Overexpression On Cytokines Profilingsupporting

confidence: 78%

Effect of NUCKS-1 Overexpression on Cytokine Profiling in Obese Women with Breast Cancer

Soliman

Zineldeen²,

El-Khadrawy³

2014

Asian Pacific Journal of Cancer Prevention

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“…Thus, we believe that the expression of NUCKS in different types of skin tumors should be considered as biomarker for tumor malignancy and not for inflammation. This remains in agreement with general findings of the other authors [7] where the most intense NUCKS staining was found in invasive ductal carcinoma compared to normal tissues. In summary, we would like to emphasize that NUCKS is overexpressed in BCC, SCC cells and it is also expressed in KA cells showing much higher expression than that of Ki67.…”

Section: Discussionsupporting

confidence: 83%

“…Some of these analyses related the occurrence of the protein to histological grade or to a particular tumor type and also to benign epithelial proliferations. Several studies performed during the last four years by independent research groups have revealed similar results in relation to significance of NUCKS expression in a number of breast lesions [7,11]. Using patient-matched samples of normal breast tissue and breast cancer by mass spectrometry we revealed elevated levels of NUCKS in protein extracts from ductal breast cancers [11].…”

Section: Introductionsupporting

confidence: 51%

“…This supports the assumption that stronger NUCKS expression may reflect higher malignancy potential of examined tumor. Drosos et al [7] found that the expression of NUCKS did not show correlation with Ki67 expression, neither in different breast lesions, including malignant tumors, nor in cell cultures. In our present study we found that the strong expression of NUCKS was observed in larger amount of cells than the expression of Ki67 and this referred to all examined skin tumors.…”

Section: Discussionmentioning

confidence: 99%

“…Until now NUCKS has been studied in detail using a variety of biochemical and cell biological methods [1,2,[7][8][9][10][11]. Some of these analyses related the occurrence of the protein to histological grade or to a particular tumor type and also to benign epithelial proliferations.…”

Section: Introductionmentioning

confidence: 99%

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The comparison of nuclear ubiquitous casein and cyclin-dependent kinases substrate (NUCKS) with Ki67 proliferation marker expression in common skin tumors

Zduniak¹,

Agrawal²,

Symonowicz³

et al. 2014

pjp

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Nuclear ubiquitous casein and cyclin-dependent kinases substrate (NUCKS) is a chromosomal protein of unknown function. Its amino acid composition and structure of its DNA binding domain resemble those of high mobility group A (HMGA) proteins which are associated with various malignancies. Since changes in expression of HMGA are considered as a marker of tumor progression, it is possible that similar changes in expression of NUCKS could be a useful tool in diagnosis of malignant skin tumors. To investigate this assumption we used specific antibodies against NUCKS for immunohistochemistry of squamous (SCC) and basal cell carcinoma (BCC) as well as keratoacanthoma (KA). We found high expression of NU-CKS in nuclei of SCC and BCC cells which exceeded expression of the well-known proliferation marker Ki67. Expression of NUCKS in benign KA was much below that of malignant tumors. With the present study and based on our previous experience we would like to suggest the NUCKS protein as a novel proliferation marker for immunohistochemical evaluation of formalin-fixed and paraffin-embedded skin tumor specimens. We would like to emphasize that NUCKS abundance in malignant skin tumors is higher than that of the well-known proliferation marker Ki67, thus allowing more precise assessment of tumor proliferation potential.

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Identification of NUCKS1 as a colorectal cancer prognostic marker through integrated expression and copy number analysis

Kikuchi

Ishikawa

Mogushi

et al. 2012

Intl Journal of Cancer

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We identified a novel prognostic biomarker for the distant metastasis of colorectal cancer (CRC) using comprehensive combined copy number and gene expression analyses. Expression of mRNA in CRC tissue was profiled in 115 patients using an Affymetrix Gene Chip, and copy number profiles were generated for 122 patients using an Affymetrix 250K Sty array. Genes showing both upregulated expression and copy number gains in cases involving distant CRC metastasis were extracted as candidate biomarkers. Expression of the candidate gene mRNA was validated in 86 patients using quantitative reverse transcription polymerase chain reaction assays. Expression of the protein encoded by the candidate gene was assessed using immunohistochemical staining of tissue from 269 patients. The relationship between protein expression and clinicopathologic features was also examined. Following combined copy number and gene expression analyses, three genes linked to distant metastasis of CRC were extracted as candidate biomarkers. The expression of NUCKS1, reportedly overexpressed in several cancers other than CRC, was significantly higher in CRC tissue than in normal tissue. Overexpression of the NUCKS1 protein in CRC cells was found to be associated with significantly worse overall survival and relapse-free survival, indicating that NUCKS1 is an independent risk factor for CRC recurrence. The overexpression of NUCKS1 in cancer cells could be used as a CRC prognostic marker and might also be a target for treatment of this disease.Colorectal cancer (CRC) is now the third most prevalent cancer and the second leading cause of cancer-related mortality worldwide. 1 In Japan, the incidence of CRC has doubled over the past 20 years such that CRC is now the second most deadly neoplastic disease. 2,3 Surgery is still the most effective treatment for CRC. Among those patients that undergo curative surgery, some develop local recurrence or distant metastases that lead to shorter survival times. 4 Distant metastasis has a critical influence on the prognosis of CRC. Clinicopathologic indicators such as the tumor node metastasis (TNM) classification system of the International Union Against Cancer (UICC) remain the standard for prognosis and provide the basis for therapeutic decision making. However, clinicopathologic indicators alone do not enable clinicians to make precise prognoses for individual CRC patients. 5 In order to develop personalized therapy regimens, it is therefore critical that researchers identify novel genes involved in distant metastasis that can serve as predictive biomarkers. 6 A particularly powerful tool for identifying potential biomarker genes for use in cancer prognosis is the microarray. 7-9 It is now possible with microarray analysis to investigate several thousand cancer-related or cancer-specific genes at once.Chromosomal structural alterations play an important role in cancer development. In CRC, copy number aberrations (CNAs), including gains on chromosomes 7, 8, 13 and 20, and losses on chromosomes 1p, 8p, 17p and 18, a...

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NUCKS overexpression in breast cancer

Cited by 41 publications

References 33 publications

Effect of NUCKS-1 Overexpression on Cytokine Profiling in Obese Women with Breast Cancer

Effect of NUCKS-1 Overexpression on Cytokine Profiling in Obese Women with Breast Cancer

The comparison of nuclear ubiquitous casein and cyclin-dependent kinases substrate (NUCKS) with Ki67 proliferation marker expression in common skin tumors

Identification of NUCKS1 as a colorectal cancer prognostic marker through integrated expression and copy number analysis

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