Nuage constituents arising from mitochondria: Is it possible?

Reunov, A. A.; Isaeva, V. V.; Au, Doris W.T.; Wu, Renren

doi:10.1046/j.1440-169x.2000.00492.x

Cited by 45 publications

(42 citation statements)

References 28 publications

(42 reference statements)

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“…This association has been described in previous studies, which gave rise to the hypothesis that the CB may originate from intermitochondrial material [12]. Other authors have suggested that the CB originates from nuclear genome products and is then supplemented by mitochondrial genome products [13]. The connection between the CB and mitochondria may also be explained by the participation of the CB in the synthesis and transport of the apocytochrome c, a type of cytochrome c isoform, which is expressed in the testis tissue [66].…”

Section: Discussionsupporting

confidence: 69%

“…The CB seems to be very important in the spermatogenesis process since mutations in some proteins located in this structure, such as tudor proteins (TDR1/MTR-1) and rat histocompatibility antigen (OX3) cause sterility in rats [46,47]. Besides of the importance of this structure, its origins are not fully understudied, and many different theories were thought aiming to understand where the CB molecular components come from nucleus [10,11], mitochondria [12,13], and/or nucleolus [14][15][16][17][18]. The nucleolus is a nuclear domain whose main function is linked to the ribosome synthesis [19].…”

Section: Discussionmentioning

confidence: 99%

“…Some authors suggest that the origin of the CB is nuclear [10,11]. Others have proposed that the CB precursor is a dense interstitial material occurring between mitochondrial clusters [12], or that it is a result of the mitochondrial products that are released in the cell cytoplasm [13]. However, still others have suggested that the CB originates from nuages, a ribonucleoproteic complex derived from the nucleolus, which migrates to the cytoplasm during earlier spermatogenesis [14][15][16][17][18].…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Morphological Changes of Mammalian Nucleoli during Spermatogenesis and Their Possible Role in the Chromatoid Body Assembling

2012

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Chromatoid body (CB) is a typical cytoplasmic organelle of germ cells, and it seems to be involved in RNA/protein accumulation for later germ-cell differentiation. Despite most of the events in mammals spermatogenesis had been widely described in the past decades and the increase in the studies related to the CB molecular composition and physiology, the origins and functions of this important structure of male germ cells are still unclear. The aims of this study were to describe the nucleolar cycle and also to find some relationship between the nucleolar organization and the CB assembling during the spermatogenesis in mammals. Cytochemical and cytogenetics analysis showed nucleolar fragmentation in post-pachytene spermatocytes and nucleolar reorganization in post-meiotic spermatids. Significant difference in the number and in the size of nucleoli between spermatogonia and round spermatids, as well as differences in the nucleolar position within the nucleus were also observed. Ultrastructural analysis showed the CB assembling in the cytoplasm of primary spermatocytes and the nucleolar fragmentation occurring at the same time. In conclusion our results suggest that the CB may play important roles during the spermatogenesis process in mammals and that its origin may be related to the nucleolar cycle during the meiotic cell cycle.

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Section: Discussionsupporting

confidence: 69%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Morphological Changes of Mammalian Nucleoli during Spermatogenesis and Their Possible Role in the Chromatoid Body Assembling

2012

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“…Some authors support the idea that CB originates from a nuclear product that runs through the pore complex into the cytoplasm of cells (Parvinen and Parvinen, 1979;Parvinen et al, 1997). Other authors claim that CB originates from the accumulation of material from the inter-mitochondrial cytoplasm of spermatids (Fawcett et al, 1970) or from mitochondrial products that were released into the cytoplasm (Reunov et al, 2000). However, other authors believe that CB is a structure derived from fragmentation and migration of nucleolar material from the nucleus to the cytoplasm (Comings and Okada, 1972;Peruquetti and Azeredo-Oliveira, 2009;Peruquetti et al, 2008Peruquetti et al, , 2010.…”

Section: Resultsmentioning

confidence: 99%

Presence of chromatoid bodies in the Rhodnius genus detected by cytochemical analysis

et al. 2016

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ABSTRACT. In addition to the epidemiological importance regarding the transmission of Chagas disease, triatomines are also important biological models for cellular studies, because they have holocentric chromosomes, post-reductional meiosis for sex chromosomes, and nucleolar persistence. Although the nucleolus is present during spermiogenesis, it was suggested that it is inactivated and all transcriptional activity necessary for cell differentiation is supported by the chromatoid body (CB). Thus, considering the importance of CB to triatomine spermatogenesis, this paper aims to analyze the presence of this organelle in the Rhodnius genus. The testicles of five adult R. neglectus and R. prolixus males were analyzed after semi- fine sections were performed, and the material was subjected to silver ion impregnation. The CB organelle was found in the spermatid of both species. Most CB studies focused on the Triatoma genus. This study described the existence of CB in Rhodnius, confirming thus the importance of this cytoplasmic organelle to spermiogenesis of these insect vectors of Chagas disease.

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“…Some authors suggest that the origin of the CB is nuclear (Parvinen and Parvinen 1979;Parvinen et al 1997). Others have proposed that the CB precursor is a dense interstitial material occurring between mitochondrial clusters (Fawcett et al 1970), or that it is a result of the mitochondrial products that are released in the cell cytoplasm (Reunov et al 2000). However, still others have suggested that the CB originates from nuages, a ribonucleoproteic complex derived from the nucleolus, which migrates to the cytoplasm during earlier spermatogenesis (Comings and Okada 1972;Andersen 1978;Andonov 1990;Peruquetti et al 2008, Peruquetti et al 2010a.…”

Section: Introductionmentioning

confidence: 99%

Relationship between the nucleolar cycle and chromatoid body formation in the spermatogenesis ofPhrynops geoffroanus(Reptilia Testudines)

Peruquetti

Taboga

Cabral

et al. 2012

Animal Cells and Systems

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The nucleolus is a distinct nuclear territory involved in the compartmentalization of nuclear functions. There is some evidence of a relationship between nuclear fragmentation during spermatogenesis and chromatoid body (CB) formation. The CB is a typical cytoplasmic organelle of haploid germ cells, and is involved in RNA and protein accumulation for later germ-cell differentiation. The goal of this study was to qualitatively and quantitatively describe the nucleolar cycle during the spermatogenesis of Phrynops geoffroanus (Reptilia Testudines), and compare this nucleolar fragmentation with CB formation in this species through the use of cytochemical and ultrastructural analysis. Qualitative analysis showed a fragmentation of the nuclear material after pachytene of the first meiotic division in the primary spermatocytes. Quantitative analysis of the nucleolar cycle revealed a significant difference in the number of nucleoli and in the size of the nucleolus between spermatogonia and early spermatids. Using ultrastructural analysis, we recorded the beginning of the CB formation process in the cytoplasm of primary spermatocytes at the same time as when nuclear fragmentation occurs. In the cytoplasm of primary spermatocytes, the CB was observed in association with mitochondrial aggregates and the Golgi complex. In the cytoplasm of early spermatids, the CB was observed in association with lipid droplets. In conclusion, our data show that the nucleolus plays a role in the CB formation process. During spermatogenesis of P. geoffroanus, the CB is involved in some important biological processes, including acrosome formation and mitochondrial migration to the spermatozoon tail and middle piece region.

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Nuage constituents arising from mitochondria: Is it possible?

Cited by 45 publications

References 28 publications

Morphological Changes of Mammalian Nucleoli during Spermatogenesis and Their Possible Role in the Chromatoid Body Assembling

Morphological Changes of Mammalian Nucleoli during Spermatogenesis and Their Possible Role in the Chromatoid Body Assembling

Presence of chromatoid bodies in the Rhodnius genus detected by cytochemical analysis

Relationship between the nucleolar cycle and chromatoid body formation in the spermatogenesis ofPhrynops geoffroanus(Reptilia Testudines)

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