NTRC Effects on Non-Photochemical Quenching Depends on PGR5

Naranjo, Belén; Penzler, Jan-Ferdinand; Rühle, Thilo; Leister, Dario

doi:10.3390/antiox10060900

Cited by 13 publications

(15 citation statements)

References 47 publications

(93 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…g H + can be estimated through inverse of the lifetime of the rapid decay signal, upon light-to-dark transitions, of carotenoid absorption changes at 518 or 520 nm in the thylakoid membranes, which is called the electrochromic shift ( Baker et al, 2007 ). By contrast to above arguments about the CET-related function of PGR5, the role of PGR5 in CET is supported by the identification of PGR5-interacting proteins such as PGRL1, Cyt b 6 f , and NTRC ( DalCorso et al, 2008 ; Hertle et al, 2013 ; Nikkanen et al, 2018 ; Naranjo et al, 2021 ; Wu et al, 2021 ). Suppression of LEF in the Δ 5 mutant ( Suorsa et al, 2016 ) and no relationship between elevated g H + and chloroplast ATP synthase activity in the pgr5 mutant ( Yamamoto and Shikanai, 2020 ) suggest that PGR5 is related to the photosynthetic CET.…”

Section: H + -Dependent Transporters and Regulator...mentioning

confidence: 83%

Chloroplast pH Homeostasis for the Regulation of Photosynthesis

Trinh

Masuda

2022

Front. Plant Sci.

View full text Add to dashboard Cite

The pH of various chloroplast compartments, such as the thylakoid lumen and stroma, is light-dependent. Light illumination induces electron transfer in the photosynthetic apparatus, coupled with proton translocation across the thylakoid membranes, resulting in acidification and alkalization of the thylakoid lumen and stroma, respectively. Luminal acidification is crucial for inducing regulatory mechanisms that protect photosystems against photodamage caused by the overproduction of reactive oxygen species (ROS). Stromal alkalization activates enzymes involved in the Calvin–Benson–Bassham (CBB) cycle. Moreover, proton translocation across the thylakoid membranes generates a proton gradient (ΔpH) and an electric potential (ΔΨ), both of which comprise the proton motive force (pmf) that drives ATP synthase. Then, the synthesized ATP is consumed in the CBB cycle and other chloroplast metabolic pathways. In the dark, the pH of both the chloroplast stroma and thylakoid lumen becomes neutral. Despite extensive studies of the above-mentioned processes, the molecular mechanisms of how chloroplast pH can be maintained at proper levels during the light phase for efficient activation of photosynthesis and other metabolic pathways and return to neutral levels during the dark phase remain largely unclear, especially in terms of the precise control of stromal pH. The transient increase and decrease in chloroplast pH upon dark-to-light and light-to-dark transitions have been considered as signals for controlling other biological processes in plant cells. Forward and reverse genetic screening approaches recently identified new plastid proteins involved in controlling ΔpH and ΔΨ across the thylakoid membranes and chloroplast proton/ion homeostasis. These proteins have been conserved during the evolution of oxygenic phototrophs and include putative photosynthetic protein complexes, proton transporters, and/or their regulators. Herein, we summarize the recently identified protein players that control chloroplast pH and influence photosynthetic efficiency in plants.

show abstract

Section: H + -Dependent Transporters and Regulator...mentioning

confidence: 83%

Chloroplast pH Homeostasis for the Regulation of Photosynthesis

Trinh

Masuda

2022

Front. Plant Sci.

View full text Add to dashboard Cite

show abstract

“…The PGRL1 protein interacts with PGR5, and plants that are devoid of PGRL1 (in the pgrl1ab mutant) fail to accumulate PGR5 and generally show a pgr5 -like phenotype ( DalCorso et al, 2008 ). PGRL1 contains six redox-active cysteines (Cys), and is a target for thioredoxin-mediated redox regulation of the CEF pathway ( Hertle et al, 2013 ; Okegawa et al, 2020 ; Wolf et al, 2020 ; Naranjo et al, 2021 ). PGR5, however, has only one Cys residue, which is not essential for its functionality in Chlamydomonas reinhardtii ( Buchert et al, 2020 ).…”

Section: Introductionmentioning

confidence: 99%

Commonalities and specialties in photosynthetic functions of PROTON GRADIENT REGULATION5 variants in Arabidopsis

et al. 2022

Self Cite

View full text Add to dashboard Cite

The PROTON GRADIENT REGULATION5 (PGR5) protein is required for trans-thylakoid proton gradient formation and acclimation to fluctuating light. PGR5 functionally interacts with two other thylakoid proteins, PGR5-like 1 (PGRL1) and 2 (PGRL2); however, the molecular details of these interactions are largely unknown. In the Arabidopsis (Arabidopsis thaliana) pgr5-1 mutant, the PGR5G130S protein accumulates in only small amounts. In this work, we generated a knockout allele of PGR5 (pgr5-Cas) using CRISPR-Cas9 technology. Like pgr5-1, pgr5-Cas is seedling-lethal under fluctuating light, but photosynthesis and particularly cyclic electron flow (CEF), as well as chlorophyll content, are less severely affected in both pgr5-Cas and pgrl1ab (which lacks PGRL1 and PGR5) than in pgr5-1. These differences are associated with changes in the levels of 260 proteins, including components of the Calvin-Benson cycle, PSII and PSI, and the NDH complex, in pgr5-1 relative to the wild type (WT), pgr5-Cas and pgrl1ab. Some of the differences between pgr5-1 and the other mutant lines could be tentatively assigned to second-site mutations in the pgr5-1 line, identified by whole-genome sequencing. However, others, particularly the more pronounced photosynthetic defects and PGRL1 depletion (compared to pgr5-Cas), are clearly due to specific negative effects of the amino-acid substitution in PGR5G130S, as demonstrated by complementation analysis. Moreover, pgr5-1 and pgr5-Cas plants are less tolerant to long-term exposure to high light than pgrl1ab plants. These results imply that, in addition to the previously reported necessity of PGRL1 for optimal PGR5 function, PGR5 is required alongside PGRL1 to avoid harmful effects on plant performance.

show abstract

“…Redox regulation of linear photosynthetic electron transport and alternative pathways has been reported previously (Johnson, 2003). Courteille and coworkers (2013) showed that cyclic electron transport involving the NDH complex was altered in Trx m4 mutants, Nikkanen et al (2018) reported the involvement of NTRC in the control of NDH complex-dependent and Naranjo et al (2021) in PGR5-dependent cyclic flow. Both alternative electron transport pathways, cyclic and pseudocyclic flow, are in competition and down-regulation of cyclic flow is therefore supposed to stimulate pseudocyclic flow.…”

Section: Discussionmentioning

confidence: 99%

A complex and dynamic redox network regulating oxygen reduction at photosystem I

Hani,

Naranjo,

Shimakawa

et al. 2023

Preprint

Self Cite

View full text Add to dashboard Cite

Thiol-dependent redox regulations of enzyme activities play a central role in regulating photosynthesis. Beside the regulation of metabolic pathways, alternative electron transport has been shown to be subjected to thiol-dependent regulation. We investigated the regulation of O2reduction at photosystem I. The level of O2reduction in leaves and isolated thylakoid membranes depends on the photoperiod in which plants are grown. We used a set of Arabidopsis mutant plants affected in the stromal, membrane and lumenal thiol network to study the redox protein partners involved in regulating O2reduction. Light-dependent O2reduction was determined in leaves and in thylakoids of plants grown in short day and long day conditions using a spin-trapping EPR assay. In wild type samples from short day, ROS generation was twice the amount of that in samples from long day, while this difference was abolished in several redoxin mutants. An in vitro reconstitution assays showed that thioredoxin m, NADPH-dependent reductase C (NTRC) and NADPH are required for high O2reduction levels in long day thylakoids. Using isolated photosystem I, we also show that reduction of a PSI protein is responsible for the increase in O2reduction. Furthermore, differences in the membrane localization of thioredoxins m and 2-Cys peroxiredoxin were demonstrated between thylakoids of short day and long day plants. Finally, we propose a model of redox regulation of O2reduction according to the reduction power of the stroma and the capabilities of the different thiol-containing proteins to form a network of redox interactions.

show abstract

NTRC Effects on Non-Photochemical Quenching Depends on PGR5

Cited by 13 publications

References 47 publications

Chloroplast pH Homeostasis for the Regulation of Photosynthesis

Chloroplast pH Homeostasis for the Regulation of Photosynthesis

Commonalities and specialties in photosynthetic functions of PROTON GRADIENT REGULATION5 variants in Arabidopsis

A complex and dynamic redox network regulating oxygen reduction at photosystem I

Contact Info

Product

Resources

About