NS3/4A helicase inhibitory alkaloids from <i>Aptenia cordifolia</i> as HCV target

Said, Asmaa Abo Elgoud; Afifi, Ahmed H.; Ali, Taha F. S.; Samy, Mamdouh Nabil; Abdelmohsen, Usama Ramadan; Fouad, Mostafa A.; Attia, Eman Zekry

doi:10.1039/d1ra06139a

Cited by 5 publications

(3 citation statements)

References 26 publications

(25 reference statements)

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“…Generally, the process to isolate alkaloids post-extraction begins with liquid–liquid extraction by changing their partition coefficients with variation of the pH of the medium. Then, preparative thin layer chromatography (TLC), 59,60 column chromatography (commonly with a silica gel stationary phase), 61,62 preparative liquid chromatography (LC) 63–65 or a combination of them 66,67 are performed. Nowadays, an interesting post-extraction technique used for the isolation and purification of alkaloids is high-speed countercurrent chromatography (HSCCC).…”

Section: Traditional Workflow In the Study Of Alkaloidsmentioning

confidence: 99%

Sustainable approaches for the study of alkaloids from plants using supercritical fluid-based processes

et al. 2022

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Section: Traditional Workflow In the Study Of Alkaloidsmentioning

confidence: 99%

Sustainable approaches for the study of alkaloids from plants using supercritical fluid-based processes

et al. 2022

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“…Mesembryanthemum crystallinum Linn. is a medicinal and edible halophyte that originates from South Africa [30,31], while Aptenia cordifolia (L. f.) Schwant, which is also known as Mesembryanthemum cordifolium L. f., is a salt-tolerant succulent with potential medicinal value that is endemic to South Africa [32][33][34]. M. crystallinum and A. cordifolia were used in this study in order to examine the effect of biochar and BOF on plant growth, soil physicochemical properties, and soil bacteria communities in saline-alkali land and to explore the feasibility of using these components to improve the soil.…”

Section: Introductionmentioning

confidence: 99%

Impact of Biochar and Bioorganic Fertilizer on Rhizosphere Bacteria in Saline–Alkali Soil

Zhang

Liang

et al. 2022

Microorganisms

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Biochar and bioorganic fertilizers (BOF) that are used in agriculture can, both directly and indirectly, impact rhizosphere soil microorganisms. However, changes to the halophyte rhizosphere bacterial community after applying biochar and BOF to saline–alkali soil have not been thoroughly described. This study has investigated the bacterial communities of halophytes in saline–alkali soil through the addition of different biochar and BOF formulas using Illumina-based sequencing of the 16S rRNA gene fragment. B_BOF (biochar and BOF combined application) had the best effect, either by promoting the plant growth or by improving the physical and chemical properties of the soil. The concentration of the rhizosphere bacterial communities correlated with the changes in soil organic matter (OM) and organic carbon (OC). Proteobacteria, Actinobacteria, Chloroflexi, and Acidobacteria accounted for >80% of the total bacteria in each treatment. In addition, the abundance of Micromonospora was much higher in response to B_BOF than to the other treatments. BOF, with or without biochar, significantly influenced the bacterial community composition in the saline–alkali soil. The OC, OM, total nitrogen, and the available phosphorus had significant effects on the bacterial structure of this soil. The complex correlation of the bacterial communities between CK and B_BOF was higher compared to that between CK and FB or between CK and BOF. These findings suggested that the plant growth, the soil characteristics, and the diversity or community composition of the rhizosphere bacteria in saline–alkali soil were significantly influenced by B_BOF, followed by BOF, and then biochar; fine biochar had a stronger effect than medium or coarse biochar. This study provides an insight into the complex microbial compositions that emerge in response to biochar and BOF.

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“…These targets are protease (NS3), RNA-dependent RNA polymerase (NS5B), helicase (NS3), and the NS3–NS4A protein allosteric pocket located at the interface among the protease domain and helicase domain. 11 …”

Section: Introductionmentioning

confidence: 99%

NS3 helicase inhibitory potential of the marine sponge Spongia irregularis

et al. 2022

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NS3/4A helicase inhibitory alkaloids from Aptenia cordifolia as HCV target

Abstract: Chemical investigation of Aptenia cordifolia roots extract, using chromatographic and spectroscopic techniques, resulted in isolation and identification of eight known compounds.

Cited by 5 publications

References 26 publications

Sustainable approaches for the study of alkaloids from plants using supercritical fluid-based processes

Sustainable approaches for the study of alkaloids from plants using supercritical fluid-based processes

Impact of Biochar and Bioorganic Fertilizer on Rhizosphere Bacteria in Saline–Alkali Soil

NS3 helicase inhibitory potential of the marine sponge Spongia irregularis

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