Nrf2-mediated activation of HO-1 is required in the blocking effect of compound K, a ginseng saponin metabolite, against oxidative stress damage in ARPE-19 human retinal pigment epithelial cells

Park, Cheol; Cha, Hee‐Jae; Song, Kyoung-Seob; Kim, Heui‐Soo; Bang, EunJin; Lee, Hyesook; Jin, Cheng‐Yun; Kim, Gi‐Young; Choi, Yung Hyun

doi:10.1016/j.jgr.2022.09.007

Cited by 9 publications

(5 citation statements)

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“…However, excessive active oxygen can have predominantly negative effects on biological systems. High concentrations of ROS directly interact with lipids, proteins, and DNA in cells, causing damage and even cell death (Guo and DiPietro, 2010;Jiang et al, 2011;Dunnill et al, 2017;Cheng et al, 2023;Park et al, 2023). CeO 2 has been thoroughly explored for its controlled synthesis and catalytic uses due to its plentiful oxygen vacancies and reversible transition between Ce (iii) and Ce (iv) 3.…”

Section: Bioevaluation Resultsmentioning

confidence: 99%

In vitro and in vivo evaluation of alginate hydrogel-based wound dressing loaded with green chemistry cerium oxide nanoparticles

Zhao,

Wan

et al. 2023

Front. Chem.

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Interactive wound dressings have displayed promising outcomes in enhancing the wound healing process. This study focuses on creating a nanocomposite wound dressing with interactive and bioactive properties, showcasing potent antioxidant effects. To achieve this, we developed cerium oxide nanoparticles utilizing curcumin as both the reducing and capping agent. Characterization techniques such as SEM, EDX, DLS, Zetasizer, FTIR, and XRD were utilized to analyze the cerium oxide nanoparticles synthesized through a green approach. The image analysis on the obtained TEM images showed that the curcumin-assisted biosynthesized CeO2NPs have a size of 18.8 ± 4.1 nm. The peaks located at 28.1, 32.7, 47.1, 56.0, 58.7, 69.0, and 76.4 correspond to (111), (200), (220), (311), (222), (400), and (331) crystallographic planes. We applied the Debye–Scherrer equation and observed that the approximate crystallite size of the biosynthesized NPs is around 8.2 nm based on the most intensive broad Bragg peak at 28.1°. The cerium oxide nanoparticles synthesized were integrated into an alginate hydrogel matrix, and the microstructure, porosity, and swelling behavior of the resulting wound dressing were assessed. The characterization analyses provided insights into the physical and chemical properties of the green-synthesized cerium oxide nanoparticles and the alginate hydrogel-based wound dressing. In vitro studies demonstrated that the wound dressing based on alginate hydrogel exhibited favorable antioxidant properties and displayed hemocompatibility and biocompatibility. Animal studies conducted on a rat full-thickness skin wound model showed that the alginate hydrogel-based wound dressing effectively accelerated the wound healing process. Overall, these findings suggest that the alginate hydrogel-based wound dressing holds promise as a highly effective material for wound healing applications.

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Section: Bioevaluation Resultsmentioning

confidence: 99%

In vitro and in vivo evaluation of alginate hydrogel-based wound dressing loaded with green chemistry cerium oxide nanoparticles

Zhao,

Wan

et al. 2023

Front. Chem.

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“…Consistent with previous findings ( Park et al ., 2022 ; Choi, 2023 ; Park et al ., 2023 ), exposure to H 2 O 2 increased the frequency of apoptosis-induced cells in ARPE-19 cells, with loss of MMP and accumulation of ROS. As is well known, abnormal accumulation of ROS due to oxidative stress contributes to mitochondrial membrane depolarization, resulting in MMP loss indicative of mitochondrial impairment ( Bock and Tait, 2020 ; Tiwari et al ., 2022 ).…”

Section: Discussionmentioning

confidence: 99%

“…To examine the expression level of γH2AX in the nucleus, cells attached to the coverslip were treated with H 2 O 2 in the presence or absence of mangiferin, fixed with 2% paraformaldehyde, and then permeabilized with 0.1% Triton-X-100 as previously described ( Park et al ., 2023 ). The cells were probed with anti-γH2AX antibody (Ser139, Thermo Fisher Scientific) and then reacted with Alexa Fluor 594-conjugated antibody (Cell Signaling Technology).…”

Section: Methodsmentioning

confidence: 99%

Activation of Heme Oxygenase-1 by Mangiferin in Human Retinal Pigment Epithelial Cells Contributes to Blocking Oxidative Damage

Park,

Cha,

Hwangbo

et al. 2024

Biomol Ther (Seoul)

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Mangiferin is a kind of natural xanthone glycosides and is known to have various pharmacological activities. However, since the beneficial efficacy of this compound has not been reported in retinal pigment epithelial (RPE) cells, this study aimed to evaluate whether mangiferin could protect human RPE ARPE-19 cells from oxidative injury mimicked by hydrogen peroxide (H 2 O 2 ). The results showed that mangiferin attenuated H 2 O 2 -induced cell viability reduction and DNA damage, while inhibiting reactive oxygen species (ROS) production and preserving diminished glutathione (GSH). Mangiferin also antagonized H 2 O 2 -induced inhibition of the expression and activity of antioxidant enzymes such as manganese superoxide dismutase and GSH peroxidase, which was associated with inhibition of mitochondrial ROS production. In addition, mangiferin protected ARPE-19 cells from H 2 O 2 -induced apoptosis by increasing the Bcl-2/Bax ratio, decreasing caspase-3 activation, and blocking poly(ADP-ribose) polymerase cleavage. Moreover, mangiferin suppressed the release of cytochrome c into the cytosol, which was achieved by interfering with mitochondrial membrane disruption. Furthermore, mangiferin increased the expression and activity of heme oxygenase-1 (HO-1) and nuclear factor-erythroid-2 related factor 2 (Nrf2). However, the inhibition of ROS production, cytoprotective and anti-apoptotic effects of mangiferin were significantly attenuated by the HO-1 inhibitor, indicating that mangiferin promoted Nrf2-mediated HO-1 activity to prevent ARPE-19 cells from oxidative injury. The results of this study suggest that mangiferin, as an Nrf2 activator, has potent ROS scavenging activity and may have the potential to protect oxidative stress-mediated ocular diseases.

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“…Rh2 inhibited the oxidative stress of porcine oocytes induced by H 2 O 2 by regulating SIRT1 expression and mitochondrial activity [ 35 ]. CK protected human retinal pigment epithelial (RPE) cells from oxidative stress-stimulated damage by activating the Nrf2/HO-1 signaling [ 36 ]. PTS well improved DNA damage and excessive activation of the DNA repair enzyme PARP-1 and inhibited depletion of the intracellular substrate NAD+ of endothelial cells induced by H2O2, which also reversed the decrease of the ATP/ADP ratio and the GSH/GSSG ratio by increasing the activities of GR and glutathione peroxidase (GSH-Px) [ 37 ].…”

Section: Pharmacological Effects and Mechanisms Of Pds And Ptsmentioning

confidence: 99%

Structural Characters and Pharmacological Activity of Protopanaxadiol‐Type Saponins and Protopanaxatriol‐Type Saponins from Ginseng

Zhang,

Gao,

Yang

et al. 2024

Advances in Pharmacological and Pharmaceutical Sciences

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Ginseng has a long history of drug application in China, which can treat various diseases and achieve significant efficacy. Ginsenosides have always been deemed important ingredients for pharmacological activities. Based on the structural characteristics of steroidal saponins, ginsenosides are mainly divided into protopanaxadiol‐type saponins (PDS, mainly including Rb1, Rb2, Rd, Rc, Rh2, CK, and PPD) and protopanaxatriol‐type saponins (PTS, mainly including Re, R1, Rg1, Rh1, Rf, and PPT). The structure differences between PDS and PTS result in the differences of pharmacological activities. This paper provides an overview of PDS and PTS, mainly focusing on their chemical profile, pharmacokinetics, hydrolytic metabolism, and pharmacological activities including antioxidant, antifatigue, antiaging, immunodulation, antitumor, cardiovascular protection, neuroprotection, and antidiabetes. It is intended to contribute to an in‐depth study of the relationship between PDS and PTS.

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Nrf2-mediated activation of HO-1 is required in the blocking effect of compound K, a ginseng saponin metabolite, against oxidative stress damage in ARPE-19 human retinal pigment epithelial cells

Cited by 9 publications

References 35 publications

In vitro and in vivo evaluation of alginate hydrogel-based wound dressing loaded with green chemistry cerium oxide nanoparticles

In vitro and in vivo evaluation of alginate hydrogel-based wound dressing loaded with green chemistry cerium oxide nanoparticles

Activation of Heme Oxygenase-1 by Mangiferin in Human Retinal Pigment Epithelial Cells Contributes to Blocking Oxidative Damage

Structural Characters and Pharmacological Activity of Protopanaxadiol‐Type Saponins and Protopanaxatriol‐Type Saponins from Ginseng

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