Nrf2/HO-1-inductive Preventive Effect of 3,5-dicaffeoylquinic Acid, Antioxidant Compound from Green Coffee Beans, on Dimethylnitrosamine-induced Liver Fibrosis in Rats

Abstract

In this study, we investigated the hepatoprotective properties of 3,5-dicaffeoylquinic acid (DQA), a polyphenolic compound from green coffee beans, against dimethylnitrosamine (DMN)-induced hepatic fibrosis rat models. DQA, up to 10 μM concentration, did not exhibit any cytotoxic effect on Chang liver cells and Huh7 cells. DMN-treated rats showed typical biological symptoms of hepatic fibrosis, i.e., loss of body weight and pathological elevation of serum biomarkers for hepatic function. However, oral administration of DQA recovered body weight, and maintained serum biochemical markers such as albumin, bilirubin, ALP, ALT and AST in the normal range. Notably, histological and western assay proved DQA significantly alleviated collagen accumulation (α-smooth muscle actin, alpha 1 collagen type I) in liver tissue. The upregulated tissue expression of erythroid 2-related factor 2 (Nrf2) and heme oxygenase-1 (HO-1) suggested DQA upregulated the representative antioxidant enzymes and ameliorated the progression of hepatic fibrosis in DMN-treated rats.

Anti‐inflammatory potential of stevia residue extract against uric acid‐associated renal injury in mice

“…Zhao et al (2019) documented that STR enriched with chlorogenic acids and flavonoids ameliorates D‐galactose‐induced oxidative stress in aging mice by the Akt/Nrf2/HO‐1 pathway. The quercetin, dihydroquercetin, chlorogenic acid, and 3, 5‐dicaffeoylquinic acid were also reported to prevent renal injury via the Nrf2 pathway (Bao et al, 2018; Kim et al, 2020; Peng et al, 2021; Wang et al, 2020).…”

“…HO-1 pathway. The quercetin, dihydroquercetin, chlorogenic acid, and 3, 5-dicaffeoylquinic acid were also reported to prevent renal injury via the Nrf2 pathway (Bao et al, 2018;Kim et al, 2020;Peng et al, 2021;Wang et al, 2020).…”