NPY in rat retina is present in neurons, in endothelial cells and also in microglial and Müller cells

Abstract

NPY is present in the retina of different species but its role is not elucidated yet. In this work, using different rat retina in vitro models

Neuropeptide Y Receptors Y1and Y2are Present in Neurons and Glial Cells in Rat Retinal Cells in Culture

“…26 In the present study We carried out immunohistochemistry studies in adult rat retina slices ( NPY Y 1 and Y 2 receptor-ir were detected in cells which were immunoreactive for TUJ1, a neuronal marker (Figs. 3A, 3B).…”

“…24 In human retinal pigment epithelium (RPE), mRNA encoding for NPY Y 1 , Y 2 , and Y 5 receptors has been detected, while in bovine RPE only mRNAs encoding for NPY Y 1 and Y 2 receptors have been found. 25 Although the presence of NPY receptors in the rat retina has been described, in particular in NPY Y 1 , Y 2 , Y 4 , and Y 5 mRNA expression, 26,27 little is known about their localization. The NPY Y 1 receptor-ir is localized in horizontal and amacrine cell bodies and involved in their processes, 28 while NPY Y 2 receptor can be found in terminals of rod bipolar cells.…”

“…More specifically, NPY induces retinal cell proliferation through the activation of NPY Y 1 , Y 2 , and Y 5 receptors, 29 and also inhibits the [Ca 2þ ] i increase by the activation of NPY Y 1 , Y 4 , and Y 5 receptors in rat retinal cells. 30 The involvement of several NPY receptors in the same NPY effect, 26,[29][30][31] and the shortage of information on the distribution of NPY receptors in the retina have led us to characterize the localization of NPY receptors in the different cell types …”

Neuropeptide Y protects retinal neural cells against cell death induced by ecstasy

“…Moreover, it was recently described that NPY has a protective role against methamphetamine-induced neuronal apoptosis in the mouse striatum (Thiriet et al, 2005). NPY and its receptors are present in the retina of different mammalian and non-mammalian species (Bruun et al, 1986;Jen et al, 1994;Hutsler and Chalupa, 1995;Oh et al, 2002;D'Angelo and Brecha, 2004;Álvaro et al, 2007). Recently we showed that primary cell cultures of rat retinal neural cells express NPY and NPY receptors (Álvaro et al, 2007).…”

Inflammatory and Angiogenic Abnormalities in Diabetic Wound Healing: Role of Neuropeptides and Therapeutic Perspectives

“…NPY regulates multiple physiological processes including hypothalamic control of food intake, anxiety [128], and modulate the immune function [129]. NPY is not only expressed in the nervous system, but also by the heart, immune cells and EC [130][131][132][133][134]. The NPY acts trough NPY receptors.…”