Nps1/Sth1p, a component of an essential chromatin‐remodeling complex of <i>Saccharomyces cerevisiae</i>, is required for the maximal expression of early meiotic genes

Yukawa, Masashi; Katoh, Soko; Miyakawa, Tokichi; Tsuchiya, Eiko

doi:10.1046/j.1365-2443.1999.00242.x

Cited by 29 publications

(25 citation statements)

References 75 publications

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“…Other observations do however connect the RSC complex with transcription. Some rsc mutations a ect the expression of the CHA1 gene as well as certain genes involved in early meiosis (Moreira and Holmberg, 1999;Yukawa et al, 1999). The RSC complex also appears to cooperate with histone acetylases.…”

Section: Swi/snf Complexes In Saccharomyces Cerevisiaementioning

confidence: 99%

When the SWI/SNF complex remodels … the cell cycle

2001

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Mammalian cells contain several chromatin-remodeling complexes associated with the Brm and Brg1 helicaselike proteins. These complexes likely represent the functional homologs of the SWI/SNF and RSC complexes found in Saccharomyces cerevisiae. The mammalian chromatin-remodeling complexes are involved in both activation and repression of a variety of genes. Several lines of evidence also indicate that they play a speci®c role in the regulation of cell growth. Brm is down-regulated by ras signaling and its forced reexpression suppresses transformation by this oncogene. Besides, the Brg1 gene is silenced or mutated in several tumors cell lines and a Brg1-associated complex was recently found to co-purify with BRCA1, involved in breast and ovarian cancers. Finally, the gene encoding SNF5/Ini1, a subunit common to all mammalian SWI/ SNF complexes, is inactivated in rhabdoid sarcomas, a very aggressive form of pediatric cancer. The current review will address observations made upon inactivation of Brm, Brg1 and SNF5/Ini1 by homologous recombination in the mouse, as well as the possible implication of these factors in the regulation of the Retinoblastoma pRb-mediated repression of the transcription factor E2F. Oncogene (2001) 20, 3067 ± 3075.

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Section: Swi/snf Complexes In Saccharomyces Cerevisiaementioning

confidence: 99%

When the SWI/SNF complex remodels … the cell cycle

2001

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“…3) We observed previously that nps1-105, rsc1Á or rsc2Á mutation shows a notable decrease and delay in the expression of IME2 with a slight decrease in IME1 expression. 10,11) Northern blot analysis of the expression of these two early-meiotic genes in WT (W303D), nps1-105 (WTH1D) and nps1-13 (WHK13-D) strains carrying each plasmid were grown to early log phase in a minimal selection medium followed by cultivation in YPAc to early stationary phase. The cells were then shifted to sporulation medium and samples were removed at the indicated times to monitor sporulation.…”

Section: Resultsmentioning

confidence: 99%

“…Our previous studies showed that the homozygous diploid of the temperature sensitive nps1-105 mutant or the deletion allele of either the RSC1 or the RSC2 genes showed reduced expression of several early-meiotic genes, and sporulates poorly. 10,11) The nps1-105 mutation harbors an amino acid substitution within the ATPase domain, and the RSC1 and the RSC2 genes encode additional components of RSC.…”

Section: Rsc Nucleosome-remodeling Complex Plays Prominent Roles In Tmentioning

confidence: 99%

“…RNA preparation and Northern blots were carried out as described. 10) Probes for detecting DIT1, NDT80, SMK1, SPO70, SPO73, SPS100, and SWM1 mRNA were prepared as follows: the DNA fragment of each gene was amplified by PCR using the oligonucleotides listed in Table 1 with genomic DNA as a template, labeled with 32 P using the Multiprime DMA labeling kit (Amersham) and 32 PdCTP. For SWM1 probe preparation, a set of primers, SWM12-U and SWM12-R, was used.…”

Section: )mentioning

confidence: 99%

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RSC Nucleosome-remodeling Complex Plays Prominent Roles in Transcriptional Regulation throughout Budding Yeast Gametogenesis

Koyama

Nagao

Inai

et al. 2004

Bioscience, Biotechnology, and Biochemistry

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RSC is a nucleosome-remodeling complex of Saccharomyces cerevisiae essential for growth that can alter histone-DNA interaction by using the energy of ATP hydrolysis. Nps1p/Sth1p is an ATPase subunit of RSC. A mutation in the conserved ATPase domain of Nps1p causes a sporulation defect with decreased expression of early meiotic genes, especially IME2. This defect is partially suppressed by the overexpression of either IME1 or IME2. A homozygous diploid of a novel temperature-sensitive nps1 mutation, nps1-13, harboring amino acid substitutions within the bromodomain, was unable to sporulate. Overexpression of IME, IME2, or both of these genes allowed the completion of meiosis I and meiosis II in nps1-13 but not the formation of mature asci. In nps1-13 carrying YEpIME1, the expression of a group of sporulation-specific genes, which express at the middle stages of sporulation and are required for spore-wall formation, notably diminished, and several late sporulation genes expressed at the early stages of sporulation. These results suggest that Nps1p/ RSC plays important roles during the spore development process by controlling gene expression for initiating both meiosis and spore morphogenesis, and ensures proper expression timing of late meiotic genes.

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“…[8][9][10] Previous studies have indicated that acceleration of histone H3 acetylation at URS1 by Gcn5/HAT is essential for the activation of IME2, in addition to Ime1 and the ATP-dependent chromatin-remodeling complex RSC. 11,12) The molecular mechanisms underlying the transition from hypoacetylated repressive chromatin state maintained by the Rpd3-Sin3/HDAC complex to hyperacetylated active chromatin by Gcn5 at the URS1 of IME2 have been of much interest, because URS1 was bound by Ume6 under all conditions tested. It has long been thought that the binding of Ime1 to Ume6 excludes or inhibits the HDAC complex to allow H3 acetylation by Gcn5.…”

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confidence: 99%