NPR1-Independent Activation of Immediate Early Salicylic Acid-Responsive Genes in <i>Arabidopsis</i>

Uquillas, Carolina; Letelier, Ingrid; Blanco, Francisca; Jordana, Xavier; Holuigue, Loreto

doi:10.1094/mpmi.2004.17.1.34

Cited by 101 publications

(103 citation statements)

References 54 publications

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“…5), suggesting this motif may be active. In addition, the glucosyltransferase UGT73B1 (At4g34138) identified by our microarray studies contains an as-1-like element in its upstream region and was also shown recently to be induced by 2,4-D and salicylic acid (88), suggesting a potential role for these elements in the activation of some Arabidopsis glucosyltransferase promoters during xenobiotic stress. Inspection of the upstream regions for the remaining 10 UGTs shown in Table III revealed only one additional UGT gene (UGT75B1; At1g05560) containing an as-1-like element on the "Ϫ" strand (not shown); however, steady-state mRNA levels for this UGT were unresponsive to 10 M 2,4-D treatments and exhibited only modest increases (ϳ3-fold) in response to 100 M treatments (Fig.…”

Section: Response Of Selected Genes To Xenobiotics and Inducers Of Xesupporting

confidence: 54%

Detoxification and Transcriptome Response in Arabidopsis Seedlings Exposed to the Allelochemical Benzoxazolin-2(3H)-one

Baerson

Sánchez‐Moreiras

Pedrol

et al. 2005

Journal of Biological Chemistry

176

200

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Benzoxazolin-2(3H)-one (BOA) is an allelochemical most commonly associated with monocot species, formed from the O-glucoside of 2,4-dihydroxy-2H-1,4-benzoxazin-3(4H)-one by a two-step degradation process. The capacity of Arabidopsis to detoxify exogenously supplied BOA was analyzed by quantification of the major known metabolites BOA-6-OH, BOA-6-O-glucoside, and glucoside carbamate, revealing that detoxification occurs predominantly through O-glucosylation of the intermediate BOA-6-OH, most likely requiring the sequential action of as-yet-unidentified cytochrome P450 and UDP-glucosyltransferase activities. Transcriptional profiling experiments were also performed with Arabidopsis seedlings exposed to BOA concentrations, representing I 50 and I 80 levels based on root elongation inhibition assays. One of the largest functional categories observed for BOA-responsive genes corresponded to protein families known to participate in cell rescue and defense, with the majority of these genes potentially associated with chemical detoxification pathways. Further experiments using a subset of these genes revealed that many are also transcriptionally induced by a variety of structurally diverse xenobiotic compounds, suggesting they comprise components of a coordinately regulated, broad specificity xenobiotic defense response. The data significantly expand upon previous studies examining plant transcriptional responses to allelochemicals and other environmental toxins and provide novel insights into xenobiotic detoxification mechanisms in plants.

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Section: Response Of Selected Genes To Xenobiotics and Inducers Of Xesupporting

confidence: 54%

Detoxification and Transcriptome Response in Arabidopsis Seedlings Exposed to the Allelochemical Benzoxazolin-2(3H)-one

Baerson

Sánchez‐Moreiras

Pedrol

et al. 2005

Journal of Biological Chemistry

176

200

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“…Thus, both leaf and root application of MeJA and SA led to the induced systemic GS response. This is in contrast to the B. oleracea in which only root JA application yielded a systemic response (van Dam et al, 2004).…”

Section: Discussioncontrasting

confidence: 56%

“…However, there are many exceptions to this basic framework, and recent works suggest that interactions between the JA and SA pathways may play important roles in finetuning defense responses (Takahashi et al, 2004;Flors et al, 2008;Smith et al, 2009;RobertSeilaniantz et al, 2011). SA-applications did not disturb the JA-induced GS accumulation in Brassica oleracea and Brassica nigra (van Dam et al, 2004), but attenuated in several Arabidopsis genotypes (Cipollini et al, 2004). All these studies were based on the separate pre-and post-treatment of the tissues with either SA or JA.…”

Section: Introductionmentioning

confidence: 99%

Leaf and root glucosinolate profiles of Chinese cabbage (Brassica rapa ssp. pekinensis) as a systemic response to methyl jasmonate and salicylic acid elicitation

Zang

Huang

et al. 2015

J. Zhejiang Univ. Sci. B

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Zang et al. / J Zhejiang Univ-Sci B (Biomed & Biotechnol) 2015 16(8)Abstract: Glucosinolates (GSs) are an important group of defensive phytochemicals mainly found in Brassicaceae. Plant hormones jasmonic acid (JA) and salicylic acid (SA) are major regulators of plant response to pathogen attack. However, there is little information about the interactive effect of both elicitors on inducing GS biosynthesis in Chinese cabbage (Brassica rapa ssp. pekinensis). In this study, we applied different concentrations of methyl jasmonate (MeJA) and/or SA onto the leaf and root of Chinese cabbage to investigate the time-course interactive profiles of GSs. Regardless of the site of the elicitation and the concentrations of the elicitors, the roots accumulated much more GSs and were more sensitive and more rapidly responsive to the elicitors than leaves. Irrespective of the elicitation site, MeJA had a greater inducing and longer lasting effect on GS accumulation than SA. All three components of indole GS (IGS) were detected along with aliphatic and aromatic GSs. However, IGS was a major component of total GSs that accumulated rapidly in both root and leaf tissues in response to MeJA and SA elicitation. Neoglucobrassicin (neoGBC) did not respond to SA but to MeJA in leaf tissue, while it responded to both SA and MeJA in root tissue. Conversion of glucobrassicin (GBC) to neoGBC occurred at a steady rate over 3 d of elicitation. Increased accumulation of 4-methoxy glucobrassicin (4-MGBC) occurred only in the root irrespective of the type of elicitors and the site of elicitation. Thus, accumulation of IGS is a major metabolic hallmark of SA-and MeJA-mediated systemic response systems. SA exerted an antagonistic effect on the MeJA-induced root GSs irrespective of the site of elicitation. However, SA showed synergistic and antagonistic effects on the MeJA-induced leaf GSs when roots and leaves are elicitated for 3 d, respectively.

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“…be:8080/PlantCARE). Interestingly, this sequence forms the core of the as-1 element found in the promoter of genes responding in a SA-dependent, NPR1-independent fashion (Uquillas et al, 2004). We additionally found several apparently novel motifs that have not yet been described as targets of known transcription factors (according to searches in the PlantCARE, TRANSFAC, and PLACE databases).…”

Section: Conserved Cis-elements Among Genes From the Same Categorymentioning

confidence: 83%

Genome-Wide Expression Profiling Arabidopsis at the Stage ofGolovinomyces cichoracearumHaustorium Formation

et al. 2008

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(T.S., K.D.)Compatibility between plants and obligate biotrophic fungi requires fungal mechanisms for efficiently obtaining nutrients and counteracting plant defenses under conditions that are expected to induce changes in the host transcriptome. A key step in the proliferation of biotrophic fungi is haustorium differentiation. Here we analyzed global gene expression patterns in Arabidopsis thaliana leaves during the formation of haustoria by Golovinomyces cichoracearum. At this time, the endogenous levels of salicylic acid (SA) and jasmonic acid (JA) were found to be enhanced. The responses of wild-type, npr1-1, and jar1-1 plants were used to categorize the sensitivity of gene expression changes to NPR1 and JAR1, which are components of the SA and JA signaling pathways, respectively. We found that the infection process was the major source of variation, with 70 genes identified as having similarly altered expression patterns regardless of plant genotype. In addition, principal component analysis (PCA) identified genes responding both to infection and to lack of functional JAR1 (17 genes) or NPR1 (18 genes), indicating that the JA and SA signaling pathways function as secondary sources of variation. Participation of these genes in the SA or JA pathways had not been described previously. We found that some of these genes may be sensitive to the balance between the SA and JA pathways, representing novel markers for the elucidation of cross-talk points between these signaling cascades. Conserved putative regulatory motifs were found in the promoter regions of each subset of genes. Collectively, our results indicate that gene expression changes in response to infection by obligate biotrophic fungi may support fungal nutrition by promoting alterations in host metabolism. In addition, these studies provide novel markers for the characterization of defense pathways and susceptibility features under this infection condition.

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NPR1-Independent Activation of Immediate Early Salicylic Acid-Responsive Genes in Arabidopsis

Cited by 101 publications

References 54 publications

Detoxification and Transcriptome Response in Arabidopsis Seedlings Exposed to the Allelochemical Benzoxazolin-2(3H)-one

Detoxification and Transcriptome Response in Arabidopsis Seedlings Exposed to the Allelochemical Benzoxazolin-2(3H)-one

Leaf and root glucosinolate profiles of Chinese cabbage (Brassica rapa ssp. pekinensis) as a systemic response to methyl jasmonate and salicylic acid elicitation

Genome-Wide Expression Profiling Arabidopsis at the Stage ofGolovinomyces cichoracearumHaustorium Formation

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