NOX1 down-regulation attenuated the autophagy and oxidative damage in pig intestinal epithelial cell following transcriptome analysis of transport stress

Li, Huari; He, Yulong; Zhang, Cheng; Ba, Tongtong; Guo, Zeheng; Zhuo, Yisha; He, Lihua; Dai, Hanchuan

doi:10.1016/j.gene.2020.145071

Cited by 6 publications

(2 citation statements)

References 41 publications

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“…BBB damage is a key event in the secondary Selenium attenuates ischemia/reperfusion injury-induced damage to the blood-brain barrier in hyperglycemia through PI3K/AKT/mTOR pathway-mediated autophagy inhibition cNS damage following stroke, which is often associated with the aberrant autophagy of brain microvascular endothelial cells (16). Studies have revealed that excessive autophagy can lead to cell dysfunction and death, as shown by the activation of the autophagy pathway following oxygen-glucose deprivation (OGd), the loss of tight junction (TJ) protein expression and the increase in endothelial cell permeability (17,18). Furthermore, it has been revealed that the inhibition of autophagy significantly reduces endothelial cell permeability.…”

Section: Introductionmentioning

confidence: 99%

Selenium attenuates ischemia/reperfusion injury‑induced damage to the blood‑brain barrier in hyperglycemia through PI3K/AKT/mTOR pathway‑mediated autophagy inhibition

Yang

et al. 2021

Int J Mol Med

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Ischemic stroke is a leading cause of mortality and disability. diabetes mellitus, characterized by hyperglycemia, is a common concomitant disease of ischemic stroke, which is associated with autophagy dysfunction and blood-brain barrier (BBB) damage following cerebral ischemia/reperfusion (I/R) injury. At present, there is no effective treatment strategy for the disease. The purpose of the present study was to explore the molecular mechanisms underlying the protective effects of selenium on the BBB following I/R injury in hyperglycemic rats. Middle cerebral artery occlusion was performed in diabetic Sprague-dawley rats. Treatment with selenium and the autophagy inhibitor 3-methyladenine significantly reduced cerebral infarct volume, brain water content and Evans blue leakage, while increasing the expression of tight junction (TJ) proteins and decreasing that of autophagy-related proteins (P<0.05). In addition, selenium increased the phosphorylation levels of PI3K, AKT and mTOR (P<0.05). A mouse bEnd.3 brain microvascular endothelial cell line was co-cultured in vitro with an MA-h mouse astrocyte-hippocampal cell line to simulate the BBB. The cells were then subjected to hyperglycemia, followed by oxygen-glucose deprivation for 1 h and reoxygenation for 24 h. It was revealed that selenium increased TJ protein levels, reduced BBB permeability, decreased autophagy levels and enhanced the expression of phosphorylated (p)-AKT/AKT and p-mTOR/mTOR proteins (P<0.05). Treatment with wortmannin (an inhibitor of PI3K) significantly prevented the beneficial effects of selenium on the BBB, whereas insulin-like growth factor 1 (a PI3K activator) mimicked the effects of selenium. In conclusion, the present findings indicated that selenium can inhibit autophagy by regulating the PI3K/AKT/mTOR signaling pathway, significantly preventing BBB damage following cerebral I/R injury in hyperglycemic conditions.

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Section: Introductionmentioning

confidence: 99%

Selenium attenuates ischemia/reperfusion injury‑induced damage to the blood‑brain barrier in hyperglycemia through PI3K/AKT/mTOR pathway‑mediated autophagy inhibition

Yang

et al. 2021

Int J Mol Med

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“…Free radical mediated oxidative stress may play a decisive role in the pathogenesis and progression of chronic metabolic diseases. Anti-oxidative therapy has been proposed as a promising and effective strategy for preventing metabolic diseases ( 58 , 59 ).…”

Section: Pharmacological Applications Of Phytochemicals In Pig Productionmentioning

confidence: 99%

Pharmacological Applications and Action Mechanisms of Phytochemicals as Alternatives to Antibiotics in Pig Production

Sun

Zhao

et al. 2021

Front. Immunol.

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Antibiotics are widely used for infectious diseases and feed additives for animal health and growth. Antibiotic resistant caused by overuse of antibiotics poses a global health threat. It is urgent to choose safe and environment-friendly alternatives to antibiotics to promote the ecological sustainable development of the pig industry. Phytochemicals are characterized by little residue, no resistance, and minimal side effects and have been reported to improve animal health and growth performance in pigs, which may become a promising additive in pig production. This paper summarizes the biological functions of recent studies of phytochemicals on growth performance, metabolism, antioxidative capacity, gut microbiota, intestinal mucosa barrier, antiviral, antimicrobial, immunomodulatory, detoxification of mycotoxins, as well as their action mechanisms in pig production. The review may provide the theoretical basis for the application of phytochemicals functioning as alternative antibiotic additives in the pig industry.

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The postoperative cognitive dysfunction induced by central inflammation with possible involvement of the gut-brain axis

Lin¹,

Wang²,

Wang³

et al. 2022

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NOX1 down-regulation attenuated the autophagy and oxidative damage in pig intestinal epithelial cell following transcriptome analysis of transport stress

Cited by 6 publications

References 41 publications

Selenium attenuates ischemia/reperfusion injury‑induced damage to the blood‑brain barrier in hyperglycemia through PI3K/AKT/mTOR pathway‑mediated autophagy inhibition

Selenium attenuates ischemia/reperfusion injury‑induced damage to the blood‑brain barrier in hyperglycemia through PI3K/AKT/mTOR pathway‑mediated autophagy inhibition

Pharmacological Applications and Action Mechanisms of Phytochemicals as Alternatives to Antibiotics in Pig Production

The postoperative cognitive dysfunction induced by central inflammation with possible involvement of the gut-brain axis

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