Novobiocin and the differentiation of peptococci and peptostreptococci.

Abstract

SUMMARY Paper discs containing 5 /tg of novobiocin were used as a presumptive test to differenitiate peptococci and peptostreptococci. Zone diameters were measured and minimum inhibitory concentrations (MIC) of the antibiotic for each group were performed to ascertain the activity of the antibiotic against these genera. All strains of peptococci showed no zone of inhibition in the disc test together with an MIC of 25 ,ug/ml or greater. All strains of peptostreptococci showed zones of inhibition of at least 15 mm diameter together with an MIC of 1 f6 jug/ml or less.

Anaerobic Periurethral Flora of Healthy Women and Women Susceptible to Urinary-tract Infection

“…They were then inoculated onto lysed-blood medium and incubated in air and anaerobically for 3 days. All obligate anaerobes were identified by gram staining followed by antibiograms when necessary (Leigh and Simmons, 1977;Wren, Eldon and Dakin, 1977). The density of bacteria on the periurethral surface was calculated in terms of number of bacteria per cm2 by counting the number of colonies transferred from the gelatin cylinder and multiplying by a factor of 14 (Cooper et al, 1980).…”

Prolonged primary incubation in the isolation of anaerobic bacteria from clinical specimens

“…Earlier studies in this laboratory have shown that unbroken primary incubation for 48 h may be an important phase in the cultural process (Wren, et al, 1977a;Wren, 1977). The present study shows that only 29% of the cultivable anaerobic flora in a group of specimens can be isolated after incubation for 24 h and 60% after 48 h by the CONV system.…”

Effect of glucose concentration in the growth medium upon neutral and acidic fermentation end-products of Clostridium bifermentans, Clostridium sporogenes and Peptostreptococcus anaerobius

“…The anaerobes were characterised on the basis of morphology in gram-stained smears, fermentation pattern (Holdeman et al, 1977) after growth in cooked meat medium (Lab-m Ltd, Ford Lane, Salford), and result profile in API 20A (API Ltd, Farnborough, Hants;Hauser and Zabransky, 1976). Additional tests used for P. anaerobius were sensitivity to novobiocin (Wren, Eldon and Dakin, 1977) and liquoid (Wideman ef al., 1976); API 20A was not used for this organism.…”