NovelFam3000 – Uncharacterized human protein domains conserved across model organisms

Abstract

Background: Despite significant efforts from the research community, an extensive portion of the proteins encoded by human genes lack an assigned cellular function. Most metazoan proteins are composed of structural and/or functional domains, of which many appear in multiple proteins. Once a domain is characterized in one protein, the presence of a similar sequence in an uncharacterized protein serves as a basis for inference of function. Thus knowledge of a domain's function, or the protein within which it arises, can facilitate the analysis of an entire set of proteins.

SCP4-STK35/PDIK1L complex is a dual phospho-catalytic signaling dependency in acute myeloid leukemia

“…In accord with prior studies (Kemmer et al, 2006;Zhao et al, 2014;Kang et al, 2016), we found that SCP4 was localized to the nucleus of AML cells (Figure 4A). However, our efforts at performing SCP4 chromatin immunoprecipitation sequencing (ChIP-seq) in MOLM-13 cells failed to identify evidence of sequence-specific DNA occupancy (data not shown).…”

The SCP4-STK35/PDIK1L complex is a dual phospho-catalytic signaling dependency in acute myeloid leukemia

“…In accord with prior studies (Kemmer et al, 2006;Zhao et al, 2014;Kang et al, 2016), we found that SCP4 was localized to the nucleus of AML cells (Figure 4A). However, our efforts at performing SCP4 ChIP-seq in MOLM-13 cells failed to identify evidence of sequence-specific DNA occupancy (data not shown).…”

A systematic study of nuclear interactome of C-terminal domain small phosphatase-like 2 using inducible expression system and shotgun proteomics

“…It is a highly conserved and pivotal enzyme for modifying the CTD of RNA Pol II (3) . While searching for FCP1 homologs, proteins containing a region sharing homology with the catalytic domain of the 1 st identified and characterized FCP1, but not the BRCT domain, have been identified, including small CTD phosphatase 1 (SCP1), SCP2, SCP3, CTD small phosphatase like protein 2 (CTDSPL2), mitochondrial import inner membrane translocase subunit TIM50 (TIMM50), and UBLCP (4 - 7) . SCPs are transcriptional regulators that can silence neuronal genes in non-neuronal tissue in a global manner.…”