Novel yeast isolated from broilers’ feedstuff, gut and faeces as aflatoxin B<sub>1</sub>adsorbents

Magnoli, Alejandra Paola; Rodriguez, M.; Poloni, Valeria; Rojo, María Cecilia; Combina, Mariana; Chiacchiera, Stella Maris; Dalcero, Ana María; Cavaglieri, Lilia Reneé

doi:10.1111/jam.13297

Cited by 17 publications

(12 citation statements)

References 48 publications

(45 reference statements)

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“…The adsorption of mycotoxins by yeast-delivered products may be also influenced by different environmental conditions such as pH, temperature, presence of bile salts and digestive enzymes in gastrointestinal track, feed components, type of ions and their concentration in aqueous solutions, as well as intestinal microbiota activity [15,23,27,32,38,39,42]. Obtained results show lower adsorption of ZEN under near neutral and alkali conditions comparing with acidic conditions regardless of the type of preparations tested (cell walls and β -glucan).…”

Section: Discussionmentioning

confidence: 99%

“…The possibility of minimization or alleviation of mycotoxin effects on animals by yeast-delivered preparations is an area of present research with promising results [16,20,21,22,23,24]. Yeast-based feed additives may counteract the toxic effect of mould secondary metabolites by acting as adsorbents but also by modulation of local humoral cellular response [24,25,26].…”

Section: Introductionmentioning

confidence: 99%

“…Yeast-based feed additives may counteract the toxic effect of mould secondary metabolites by acting as adsorbents but also by modulation of local humoral cellular response [24,25,26]. The efficiency of toxins binding by preparations of yeast origin is correlated mostly with the composition, structure and content of polymers which form yeast cell walls [23,27,28,29,30,31]. However, accessible surphase area of the adsorbent is a crucial parameter also [5,32].…”

Section: Introductionmentioning

confidence: 99%

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Candida utilis ATCC 9950 Cell Walls and β(1,3)/(1,6)-Glucan Preparations Produced Using Agro-Waste as a Mycotoxins Trap

Bzducha-Wróbel

Bryła

Gientka

et al. 2019

Toxins

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Mycotoxins are harmful contaminants of food and feed worldwide. Feed additives with the abilities to trap mycotoxins are considered substances which regulate toxin transfer from feed to tissue, reducing its absorption in animal digestive tract. Market analysis emphasizes growing interest of feed producers in mycotoxins binders obtained from yeast biomass. The aim of the study was to prescreen cell walls (CW) and β(1,3)/(1,6)-glucan (β-G) preparations isolated from Candida utilis ATCC 9950 cultivated on waste potato juice water with glycerol as adsorbents for aflatoxin B1 (AFB1), zearalenone (ZEN), ochratoxin A (OTA), deoxynivalenol (DON), nivalenol (NIV), T-2 toxin (T-2) and fumonisin B1 (FB1). The adsorption was studied in single concentration tests at pH 3.0 and 6.0 in the presence of 1% of the adsorbent and 500 ng/mL of individual toxin. Evaluated CW and β-G preparations had the potential to bind ZEN, OTA and AFB1 rather than DON, NIV, T-2 toxin and FB1. The highest percentage of adsorption (about 83%), adsorption capacity (approx. 41 µg/ g preparation) and distribution coefficient (458.7mL/g) was found for zearalenone when CW preparation was used under acidic conditions. Higher protein content in CW and smaller particles sizes of the formulation could influence more efficient binding of ZEN, OTA, DON and T-2 toxin at appropriate pH compared to purified β-G. Obtained results show the possibility to transform the waste potato juice water into valuable Candida utilis ATCC 9950 preparation with mycotoxins adsorption properties. Further research is important to improve the binding capacity of studied preparations by increasing the active surface of adsorption.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Candida utilis ATCC 9950 Cell Walls and β(1,3)/(1,6)-Glucan Preparations Produced Using Agro-Waste as a Mycotoxins Trap

Bzducha-Wróbel

Bryła

Gientka

et al. 2019

Toxins

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“…Generally, all the listed publications report positive (mainly significant) results regarding aflatoxin control by microbes and/or enzymes. Besides the known and practically used microbes such as Saccharomyces strains, there is an abundance of promising research aiming at new candidates that are isolated from normal animal microbiota, thereby increasing the probability of colonization [56][57][58][59], while other studies focus on affordable mass production options [60]. Antimicrobial/antifungal effects of LAB were shown in the studies of Dogi et al (2013 and [61,62], and Drobná et al (2017) [57], while the synergistic effects of different LAB strains on aflatoxin degradation were published in the study of Zielinska and Fabiszewska (2018) [63].…”

Section: Microbes and Enzymesmentioning

confidence: 99%

A Systematic Review of the Efficacy of Interventions to Control Aflatoxins in the Dairy Production Chain—Feed Production and Animal Feeding Interventions

Farkas

Országh

Engelhardt

et al. 2022

Toxins

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The study presents a systematic review of published scientific articles investigating the effects of interventions aiming at aflatoxin reduction at the feed production and animal feeding phases of the milk value chain in order to identify the recent scientific trends and summarize the main findings available in the literature. The review strategy was designed based on the guidance of the systematic review and knowledge synthesis methodology that is applicable in the field of food safety. The Web of Science and EBSCOhost online databases were searched with predefined algorithms. After title and abstract relevance screening and relevance confirmation with full-text screening, 67 studies remained for data extraction, which were included in the review. The most important identified groups of interventions based on their mode of action and place in the technological process are as follows: low-moisture production using preservatives, acidity regulators, adsorbents and various microbiological additives. The results of the listed publications are summarized and compared for all the identified intervention groups. The paper aimed to help feed producers, farmers and relevant stakeholders to get an overview of the most suitable aflatoxin mitigation options, which is extremely important in the near future as climate change will likely be accompanied by elevated mycotoxin levels.

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“…P. kudriavzevii isolated from chicken feces exhibits probiotic properties in vitro [ 46 ]. With a capacity to bind to aflatoxin B1, dietary supplementation of P. kudriavzevii mitigates the adverse effect of aflatoxin B1 on the growth performance of broilers [ 47 ]. Candida species are a part of commensal mycobiota in the GI tract but may cause candidiasis in poultry and humans [ 36 , 41 , 44 ].…”

Section: Discussionmentioning

confidence: 99%

Perturbations of the ileal mycobiota by necrotic enteritis in broiler chickens

Liu

Robinson

Whitmore

et al. 2021

J Animal Sci Biotechnol

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Background Intestinal microbiota is critical for maintaining animal health and homeostasis. However, involvement of the fungal community, also known as the mycobiota, in animal health and disease is poorly understood. This study was aimed to examine the association between the intestinal mycobiota and the severity of necrotic enteritis (NE), an economically significant poultry disease. Methods A total of 90 day-of-hatch Cobb broilers were infected with Eimeria maxima on d 10, followed by an oral challenge with C. perfringens on d 14 to induce NE, while another 10 broilers were served as mock-infected controls. On d 17, the lesions in the jejunum were scored, and the ileal digesta were subjected to DNA isolation and real-time PCR quantification of total bacterial and fungi populations. Internal transcribed spacer 2 (ITS2) amplicon sequencing was also performed to profile the ileal mycobiota composition. Changes in the ileal mycobiota in response to NE were investigated. Spearman correlation analysis was further conducted to identify the correlations between relative abundances of individual ileal fungi and the severity of NE. Results While the total bacterial population in the ileum was increased by 2- to 3-fold in NE chickens, the total fungal population was progressively declined in more exacerbated NE, with the most severely infected chickens showing a nearly 50-fold reduction relative to mock-infected controls. Richness of the ileal mycobiota also tended to reduce in chickens with NE (P = 0.06). Compositionally, among 30 most abundant fungal amplicon sequence variants (ASVs), 11 were diminished and 7 were enriched (P < 0.05), while 12 remained largely unchanged in NE-afflicted chickens (P > 0.05). Multiple Wallemia and Aspergillus species were markedly diminished in NE (P < 0.05) and also showed a significant negative correlation with NE severity (P < 0.05). Conclusions Dysbiosis of the ileal mycobiota is induced evidently by NE and the extent of the dysbiosis is positively correlated with disease severity. These findings suggest a possible role of the intestinal mycobiota in NE pathogenesis and highlight the mycobiota as a new potential target for NE mitigation in poultry.

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Novel yeast isolated from broilers’ feedstuff, gut and faeces as aflatoxin B₁adsorbents

Abstract: This study makes a contribution to using native yeasts from broilers' environment for controlling chronic aflatoxicosis in avian production.

Cited by 17 publications

References 48 publications

Candida utilis ATCC 9950 Cell Walls and β(1,3)/(1,6)-Glucan Preparations Produced Using Agro-Waste as a Mycotoxins Trap

Candida utilis ATCC 9950 Cell Walls and β(1,3)/(1,6)-Glucan Preparations Produced Using Agro-Waste as a Mycotoxins Trap

A Systematic Review of the Efficacy of Interventions to Control Aflatoxins in the Dairy Production Chain—Feed Production and Animal Feeding Interventions

Perturbations of the ileal mycobiota by necrotic enteritis in broiler chickens

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Novel yeast isolated from broilers’ feedstuff, gut and faeces as aflatoxin B1adsorbents

Abstract: This study makes a contribution to using native yeasts from broilers' environment for controlling chronic aflatoxicosis in avian production.

Cited by 17 publications

References 48 publications

Candida utilis ATCC 9950 Cell Walls and β(1,3)/(1,6)-Glucan Preparations Produced Using Agro-Waste as a Mycotoxins Trap

Candida utilis ATCC 9950 Cell Walls and β(1,3)/(1,6)-Glucan Preparations Produced Using Agro-Waste as a Mycotoxins Trap

A Systematic Review of the Efficacy of Interventions to Control Aflatoxins in the Dairy Production Chain—Feed Production and Animal Feeding Interventions

Perturbations of the ileal mycobiota by necrotic enteritis in broiler chickens

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Product

Resources

About

Novel yeast isolated from broilers’ feedstuff, gut and faeces as aflatoxin B₁adsorbents