Aiming at elevating Scenedesmus obliquus biofilm growth from the perspectives of CO 2 transfer enhancement and light delivery optimization, a membrane-based photobioreactor with built-in optical fibers (OF-GP-BPBR) was proposed in this work. In particular, the S. obliquus biofilm attached to the membrane featured by gas permeability could directly assimilate the CO 2 molecules diffused across the membrane. The emergent light of the external light-emitting diode light sources was guided, diluted, and redistributed within the biofilm providing light energy for S. obliquus cell photoautotrophic proliferation. Responses of S. obliquus biofilm growth to various optical fiber quantities, CO 2 concentrations, and initial nitrate concentrations were measured. The maximum areal S. obliquus biofilm biomass density was achieved to be 37.32 g m −2 under an initial nitrate concentration of 1.18 mM, a CO 2 concentration of 10%, and an optical fiber quantity of 13. This work demonstrates a prospective approach to promote biofilm-based microalgae biomass accumulation by introducing light-guiding materials into photobioreactors.