Novel volumetric adsorptive microsampling technique for determination of perfluorinated compounds in blood

Koponen, Jani; Rudge, James; Kushon, Stuart; Kiviranta, Hannu

doi:10.1016/j.ab.2018.01.015

Cited by 16 publications

(13 citation statements)

References 26 publications

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“…Results from this study suggest that self-collection of whole blood samples using VAMSs is well suited to characterize PFAS blood concentrations and that results differ from serum by approximately a factor of two, with high correlations between PFASs measured in traditional serum samples and whole blood sampled with VAMSs. Collection of data from a relatively large number of participants (53 people) helps to demonstrate that whole blood collected and analyzed using VAMSs can be used to predict serum levels with relatively good accuracy, which was not evaluated in the prior Koponen et al (2018) study . Therefore, individuals could use the VAMS to measure their PFAS exposure and convert their values to serum concentrations to support comparisons with NHANES data.…”

Section: Resultsmentioning

confidence: 99%

“…Collection requires a phlebotomist for a blood draw, a dedicated laboratory to separate serum from whole blood, and shipment on dry ice with a biohazard designation. Dried blood spots (DBSs) using a finger or heel prick have been used for many years and overcome many of these challenges. − While this approach has been used to characterize historical exposure to environmental contaminants including PFASs, there is a loss in accuracy due to the varying volume of blood in a DBS. As they precisely meter the absorption of blood via a proprietary hydrophilic polymer, volumetric absorptive microsamplers (VAMSs) show promise in overcoming this otherwise major DBS limitation for quantitative PFAS analyses of human blood.…”

Section: Introductionmentioning

confidence: 99%

“…A prior study by Koponen et al 2018 utilized VAMSs for PFAS analysis in whole blood; however, they encountered several limitations, particularly a small sample size (12 participants in a Finnish population). 9 In addition, serum was not collected and analyzed alongside the whole blood for comparison purposes, and this study only monitored 12 PFASs, of which only four were detected in more than 50% of the samples. This study also utilized 10 μL tips to collect the whole blood, which is a very small volume and may be more prone to inaccuracies in the volume sampled.…”

Section: ■ Introductionmentioning

confidence: 99%

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Self-Collection Blood Test for PFASs: Comparing Volumetric Microsamplers with a Traditional Serum Approach

Carignan,

Bauer,

Patterson

et al. 2023

Environ. Sci. Technol.

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A remote sampling approach was developed at Eurofins for quantifying per-and polyfluoroalkyl substances (PFASs) in whole blood samples collected using volumetric absorptive microsamplers (VAMSs), which allow for self-collection of blood using a finger prick. This study compares PFAS exposure measured by self-collection of blood using VAMSs to the standard venous serum approach. Blood samples were collected from participants (n = 53) in a community with prior PFAS drinking water contamination using a venous blood draw as well as participant self-collection using VAMSs. Whole blood from the venous tubes was also loaded onto VAMSs to compare differences in capillary vs venous whole blood PFAS levels. Samples were quantified for PFASs using liquid chromatography tandem mass spectrometry and online solid-phase extraction. PFAS levels in serum were highly correlated with measurements in capillary VAMSs (r ≥ 0.91 and p < 0.05). Serum PFAS levels were generally twofold higher than whole blood, reflecting expected differences in their composition. Of interest, FOSA was detected in whole blood (both venous and capillary VAMSs) but not in serum. Overall, these findings indicate that VAMSs are useful self-collection tools for assessing elevated human exposure to PFASs.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: ■ Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Self-Collection Blood Test for PFASs: Comparing Volumetric Microsamplers with a Traditional Serum Approach

Carignan,

Bauer,

Patterson

et al. 2023

Environ. Sci. Technol.

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“…Microsampling techniques such as plasma extraction cards, VAMS, and CMS (Figs. A, B, and C) are employed to collect very small volumes (~10–100 µL) (Jonsson et al, ; Bowen et al, ; Jonsson et al, ; Chapman et al, ; White et al, ; Kar, Maiti, & Chakraborty, ; Spooner et al, ; Mercolini et al, ; Niu et al, ; Barco et al, ; van den Broek et al, ; Kita & Mano, ; Qu et al, ; Fang et al, ; Koponen et al, ), which greatly reduces exposure to biohazards, eliminates the need for a large number of satellite animals, and allows for the collection of samples from individuals who are not able to provide large sample volumes such as newborn infants, the elderly, and the critically ill (White et al, ).…”

Section: Microsamplingmentioning

confidence: 99%

Emerging trends in paper spray mass spectrometry: Microsampling, storage, direct analysis, and applications

Frey

Damon

Badu‐Tawiah

2019

Mass Spectrometry Reviews

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Recent advancements in the sensitivity of chemical instrumentation have led to increased interest in the use of microsamples for translational and biomedical research. Paper substrates are by far the most widely used media for biofluid collection, and mass spectrometry is the preferred method of analysis of the resultant dried blood spot (DBS) samples. Although there have been a variety of review papers published on DBS, there has been no attempt to unify the century old DBS methodology with modern applications utilizing modified paper and paper‐based microfluidics for sampling, storage, processing, and analysis. This critical review will discuss how mass spectrometry has expanded the utility of paper substrates from sample collection and storage, to direct complex mixture analysis to on‐surface reaction monitoring.

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“…However, when compared with partial-punch DBS approaches, VAMS, in which a fixed volume of blood is absorbed by a polymeric absorbent tip, offers several advantages: the volume absorbed is not dependent on the haematocrit, there is no potential homogeneity issue [ 12 , 13 , 14 ] and, importantly, users indicated a preference towards VAMS [ 15 ]. Although VAMS has been applied for a multitude of analytes [ 16 ], including proteins such as haemoglobin [ 17 ], β-lactoglobulin and myoglobin [ 18 ], drugs [ 19 ] and contaminants such as perfluorinated compounds [ 20 ], to our knowledge, VAMS has never been tested for multiple mycotoxins analysis.…”

Section: Introductionmentioning

confidence: 99%

Volumetric Absorptive Microsampling as an Alternative Tool for Biomonitoring of Multi-Mycotoxin Exposure in Resource-Limited Areas

Vidal

Belova

Stove

et al. 2021

Toxins

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Biomonitoring of biological samples arises as an effective tool to evaluate the exposure to mycotoxins in the population. Owing to the wide range of advantages, there is a growing interest in the use of non- and minimally invasive alternative sampling strategies, such as dried blood spot sampling or volumetric absorptive microsampling (VAMS). A VAMS-based multi-mycotoxin method was developed and validated for 24 different mycotoxins. Method validation was based on the Bioanalytical Method Validation Guideline of the Food and Drug Administration from the United States and for most of the studied mycotoxins, the results of the performance characteristics were in agreement with the criteria of the European Commission Decision 2002/657/EC. The recovery for the different mycotoxins was not haematocrit dependent and remained acceptable after storing the VAMS for 7 and 21 days at refrigeration temperature (4 °C) and room temperature, demonstrating that VAMS could be applied to assess mycotoxin exposure in blood in resource-limited areas, where there may be a delay between sampling and analysis. Finally, a comparison between VAMS and a procedure for liquid whole blood analysis, performed on 20 different blood samples, did not result in missed exposed cases for VAMS. Moreover, both methods detected similar levels of ochratoxin A, ochratoxin alpha, zearalenone and aflatoxin B1. Given all the benefits associated with VAMS and the developed method, VAMS sampling may serve as an alternative to conventional venous sampling to evaluate multiple mycotoxin exposure.

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Novel volumetric adsorptive microsampling technique for determination of perfluorinated compounds in blood

Cited by 16 publications

References 26 publications

Self-Collection Blood Test for PFASs: Comparing Volumetric Microsamplers with a Traditional Serum Approach

Self-Collection Blood Test for PFASs: Comparing Volumetric Microsamplers with a Traditional Serum Approach

Emerging trends in paper spray mass spectrometry: Microsampling, storage, direct analysis, and applications

Volumetric Absorptive Microsampling as an Alternative Tool for Biomonitoring of Multi-Mycotoxin Exposure in Resource-Limited Areas

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