Novel virus-encoded microRNA molecules expressed by ovine herpesvirus 2-immortalized bovine T-cells

Levy, Claire S.; Russell, George C.; Dalziel, Robert

doi:10.1099/vir.0.037606-0

Cited by 8 publications

(12 citation statements)

References 32 publications

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“…Eight of these were confirmed by northern hybridization [22]. Here we extend these studies using two PCR protocols to investigate the expression of the other predicted OvHV-2 miRNAs.…”

Section: Introductionmentioning

confidence: 58%

“…Using northern hybridization we have previously demonstrated the expression of the eight of the 45 predicted miRNAs that were represented by the highest number of reads in our parallel sequencing data [22]. RT-PCR using miRNA specific forward primers and a universal reverse primer for the remaining 37 predicted miRNAs initially identified 22 virus-encoded miRNAs (Table S1, Table 1).…”

Section: Resultsmentioning

confidence: 89%

“…Previous name : designation in Levy et al 2012. Abundance (RNA-seq) : Total number of sequence tags representing the miRNA in the RNA-seq data [22]. Walz et al .…”

Section: Resultsmentioning

confidence: 99%

“…Validation method : Levy et al . : validated previously [22]; Sequencing: validated using the RT-PCR and subsequent sequencing; Specific cDNA: validated using the approach of Varkonyi-Gasic et al [24]…”

Section: Resultsmentioning

confidence: 99%

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Expression of Ovine Herpesvirus -2 Encoded MicroRNAs in an Immortalised Bovine – Cell Line

et al. 2014

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Ovine herpesvirus-2 (OvHV-2) infects most sheep, where it establishes an asymptomatic, latent infection. Infection of susceptible hosts e.g. cattle and deer results in malignant catarrhal fever, a fatal lymphoproliferative disease characterised by uncontrolled lymphocyte proliferation and non MHC restricted cytotoxicity. The same cell populations are infected in both cattle and sheep but only in cattle does virus infection cause dysregulation of cell function leading to disease. The mechanism by which OvHV-2 induces this uncontrolled proliferation is unknown. A number of herpesviruses have been shown to encode microRNAs (miRNAs) that have roles in control of both viral and cellular gene expression. We hypothesised that OvHV-2 encodes miRNAs and that these play a role in pathogenesis. Analysis of massively parallel sequencing data from an OvHV-2 persistently-infected bovine lymphoid cell line (BJ1035) identified forty-five possible virus-encoded miRNAs. We previously confirmed the expression of eight OvHV-2 miRNAs by northern hybridization. In this study we used RT-PCR to confirm the expression of an additional twenty-seven OvHV-2-encoded miRNAs. All thirty-five OvHV-2 miRNAs are expressed from the same virus genome strand and the majority (30) are encoded in an approximately 9 kb region that contains no predicted virus open reading frames. Future identification of the cellular and virus targets of these miRNAs will inform our understanding of MCF pathogenesis.

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“…Eight of these were confirmed by northern hybridization [22]. Here we extend these studies using two PCR protocols to investigate the expression of the other predicted OvHV-2 miRNAs.…”

Section: Introductionmentioning

confidence: 58%

Section: Resultsmentioning

confidence: 89%

“…Previous name : designation in Levy et al 2012. Abundance (RNA-seq) : Total number of sequence tags representing the miRNA in the RNA-seq data [22]. Walz et al .…”

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

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Expression of Ovine Herpesvirus -2 Encoded MicroRNAs in an Immortalised Bovine – Cell Line

et al. 2014

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“…Therefore, ASFV-C9 with all other sequences can be considered as degradation products of viral RNA. Being aware of the reduced number of animals used, different tissues were used to generate a total of 14 RNA libraries, number that exceeds the majority of similar studies so far published, normally ranging from 1 to 4 and carried out in cell culture, and not in the natural host [29–32]. …”

Section: Discussionmentioning

confidence: 99%

African swine fever virus does not express viral microRNAs in experimentally infected pigs

et al. 2018

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BackgroundAfrican swine fever virus (ASFV) is the etiological agent of African swine fever (ASF), a re-expanding devastating and highly lethal hemorrhagic viral disease. microRNAs (miRNAs) are a new class of small non-coding RNAs that regulate gene expression post-transcriptionally. The discovery of virus specific miRNAs has increased both in number and importance in the past few years. We have recently described the differential expression of several porcine miRNAs during in vivo infection with attenuated and virulent ASFV strains. Here, we have extended these studies trying to identify the presence of viral miRNAs encoded by ASFV in an in vivo infection in pigs.ResultsSixteen small RNA libraries were analyzed from spleen and submandibular lymph nodes obtained from eight pigs, seven infected with either the virulent E75 ASFV strain or its attenuated counterpart E75CV1, or from pigs surviving E75CV1-infection and challenged with BA71 (heterologous challenge) and one non infected as negative control. Samples were recovered at different times post-infection. Libraries were analyzed by next-generation sequencing. Some viral miRNA candidates were initially identified, which did not correspond to porcine miRNAs. Further structural analyses were carried out in order to confirm if they met the conformational requirements to be considered a viral miRNA.ConclusionsThe analysis of sixteen small RNA libraries prepared from two different tissues obtained from pigs experimentally infected with E75, E75CV1 or with E75CV1 plus BA71, revealed the presence of six potential miRNA sequences but none of them met the requirements to be considered as viral miRNAs. Thus, we can conclude that ASFV does not express miRNAs in vivo, at least under the experimental conditions described here.

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Virus-Encoded microRNAs

Tuddenham

Pfeffer

2013

Encyclopedia of Molecular Cell Biology and Molecular Medicine

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Novel virus-encoded microRNA molecules expressed by ovine herpesvirus 2-immortalized bovine T-cells

Cited by 8 publications

References 32 publications

Expression of Ovine Herpesvirus -2 Encoded MicroRNAs in an Immortalised Bovine – Cell Line

Expression of Ovine Herpesvirus -2 Encoded MicroRNAs in an Immortalised Bovine – Cell Line

African swine fever virus does not express viral microRNAs in experimentally infected pigs

Virus-Encoded microRNAs

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