Novel Variant (
 bla
 VIM-11
 ) of the Metallo-β-Lactamase
 bla
 VIM
 Family in a GES-1 Extended-Spectrum-β-Lactamase-Producing
 Pseudomonas aeruginosa
 Clinical Isolate in Argentina

Pasterán, Fernando; Faccone, Diego; Petroni, Alejandro; Rapoport, Melina; Galas, Marcelo; Vazquez, M.; Procopio, Adriana

doi:10.1128/aac.49.1.474-475.2005

Cited by 57 publications

(36 citation statements)

References 8 publications

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“…The isolation of total DNA was performed as described previously (18). Strains were analyzed for bla VIM, bla IMP , bla SPM , bla KPC , bla Sme , and bla OXA genes (subgroups I, II, and III) by using the primers described previously (18,21,25,26). For bla IMI and bla NMC-A , specific primers were designed (forward, 5Ј-CAGAGCAAATGAACGATTTC-3Ј; reverse, 5Ј-TGGTACGCTAGCACGAATAC-3Ј).…”

Section: Methodsmentioning

confidence: 99%

Sensitive Screening Tests for Suspected Class A Carbapenemase Production in Species of Enterobacteriaceae

et al. 2009

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The detection of class A serine-carbapenemases among species of Enterobacteriaceae remains a challenging issue. Methods of identification for routine use in clinical microbiology laboratories have not been standardized to date. We developed a novel screening methodology suitable for countries with high basal levels of carbapenem resistance due to non-carbapenemase-mediated mechanisms and standardized several simple confirmatory methods that allow the recognition of bacteria producing class A carbapenemases, including KPC, Sme, IMI, NMC-A, and GES, by using boronic acid (BA) derivatives. A total of 28 genetically unrelated Enterobacteriaceae strains producing several class A carbapenemases were tested. Thirty-eight genetically unrelated negative controls were included. The isolates were tested against imipenem (IPM), meropenem (MEM), and ertapenem (ETP) by MIC and disk diffusion assays in order to select appropriate tools to screen for suspected carbapenemase production. It was possible to differentiate class A carbapenemase-producing bacteria from non-carbapenemase-producing bacteria by using solely the routine IPM susceptibility tests. The modified Hodge test was evaluated and found to be highly sensitive, although false-positive results were documented. Novel BA-based methods (a double-disk synergy test and combined-disk and MIC tests) using IPM, MEM, and ETP, in combination with 3-aminophenylboronic acid as an inhibitor, were designed as confirmatory tools. On the basis of the performance of these methods, a sensitive flow chart for suspicion and confirmation of class A carbapenemase production in species of Enterobacteriaceae was designed. By using this methodology, isolates producing KPC, GES, Sme, IMI, and NMC-A carbapenemases were successfully distinguished from those producing other classes of ␤-lactamases (extended-spectrum ␤-lactamases, AmpCs, and metallo-␤-lactamases, etc). These methods will rapidly provide useful information needed for targeting antimicrobial therapy and appropriate infection control.

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Section: Methodsmentioning

confidence: 99%

Sensitive Screening Tests for Suspected Class A Carbapenemase Production in Species of Enterobacteriaceae

et al. 2009

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“…The occurrence of bla VIM-2 in different clones and in different Pseudomonas species is indicative of the presence of horizontal gene transfer. Moreover, another study has revealed the presence of bla VIM-11 , a novel variant of the metallo-␤-lactamase bla VIM family which differs from bla VIM-2 in a unique mutation, in a Pseudomonas aeruginosa clinical isolate in the same medical center (22). Metallo-ß-lactamase-producing P. putida isolates were first detected in 2002 in Ricardo Gutierrez Children's Hospital.…”

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confidence: 99%

“…The strain was analyzed for the presence of the bla VIM, bla IMP , and bla SPM genes, using previously described primers (10,23). Additionally, according to IEF results, the strain was tested by PCR for the bla TEM , bla GES , and bla PER ß-lactamase genes (2,17,22). Thermal cycler T-gradient and MgCl 2 , deoxynucleoside triphosphate, specific primers, and Taq polymerase were supplied by Biometra, Göttingen, Germany, and Invitrogen, United States, respectively.…”

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First Case of Human Infection Due to Pseudomonas fulva , an Environmental Bacterium Isolated from Cerebrospinal Fluid

et al. 2010

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We report the first case of human infection due to Pseudomonas fulva. P. fulva caused acute meningitis following the placement of a drainage system in a 2-year-old female. Additionally, the isolate displayed a VIM-2 carbapenemase in a class 1 integron context. CASE REPORTA 2-year-old female was brought to our center after 30 days of treatment in another center due to a history of fever, malaise, and partial food rejection. A sample of urine was taken for a bacteriological culture, and empirical treatment with amoxicillin was begun. The urinalysis showed pathological sediment with more than 25 leukocytes per field, pyuria, and bacteriuria. The urinary culture yielded Ͼ10 5 CFU of Proteus penneri per ml. Based on the susceptibility test result, amoxicillin was discontinued and a 1-week course of trimethoprimsulfamethoxazole (TMP-SMX) therapy was prescribed. Since she did not recover, she was hospitalized in the same center for evaluation. After 48 h, she presented a poor general state with signs of meningismus. Therapy with ceftriaxone (150 mg/kg/ day) was started. A computerized axial tomography (CAT) test, performed after lumbar puncture, revealed ventricular dilatation. The cerebrospinal fluid (CSF) cytochemical analysis was normal, and the CSF culture was negative.Due to a torpid evolution and because the patient presented facial paralysis, she was referred to Ricardo Gutierrez Children's Hospital, where a new brain CAT was performed showing progressive hydrocephalus. In order to drain the CSF, an external catheter was placed in the lateral ventricle. A presumptive diagnosis of meningitis with a bad evolution or tuberculous meningitis was made. Empirical treatment with vancomycin (60 mg/kg/day) (intravenous [i.v.]), cefotaxime (220 mg/kg/day) (i.v.), and tuberculostatic drugs (isoniazid, 5 mg/ kg/day; rifampin, 10 mg/kg/day; streptomycin, 15 mg/kg/day; pyrazinamide, 20 mg/kg/day) was started. Since the lumbar puncture was traumatic, CSF chemistry and cell count were not carried out; however, the CSF culture obtained by lumbar puncture (for bacteria, fungi, and mycobacteria) was negative.After 9 days of having the catheter drainage in place, the patient showed no neurological sign improvement. The catheter was changed, and a CSF sample for bacterial culture was taken with negative results. The CSF cell count was 8 leukocytes/mm 3 , and protein and glucose contents were 17 mg/dl and 70 mg/dl, respectively. Vancomycin was suspended, and the patient continued with ceftriaxone, and despite a negative result for the detection of Mycobacterium tuberculosis by PCR, the patient continued on tuberculosis drugs until the culture was finished.As indicated by neurosurgery, a nuclear magnetic resonance test was carried out, showing a lesion that occupied the midbrain and pons consistent with a probable ependymoma.On the eighth day after the new drainage system was placed, the patient was in very poor condition; therefore, internal ventriculoperitoneal shunting and performing a brain biopsy of the mass were considered. A new...

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“…Addition of 4 mmol/L APB resulted in inhibition of carbapenemase activity of K. pneumoniae M9171, C. freundii M9169, both transconjugants, and K. pneumoniae D5/07, but not a VIM-11 control run in parallel (10).…”

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Klebsiella pneumoniaeCarbapenemase–2, Buenos Aires, Argentina

Pasterán

Otaegui

Guerriero

et al. 2008

Emerg. Infect. Dis.

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Novel Variant ( bla _VIM-11 ) of the Metallo-β-Lactamase bla _VIM Family in a GES-1 Extended-Spectrum-β-Lactamase-Producing Pseudomonas aeruginosa Clinical Isolate in Argentina

Cited by 57 publications

References 8 publications

Sensitive Screening Tests for Suspected Class A Carbapenemase Production in Species of Enterobacteriaceae

Sensitive Screening Tests for Suspected Class A Carbapenemase Production in Species of Enterobacteriaceae

First Case of Human Infection Due to Pseudomonas fulva , an Environmental Bacterium Isolated from Cerebrospinal Fluid

Klebsiella pneumoniaeCarbapenemase–2, Buenos Aires, Argentina

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Novel Variant ( bla VIM-11 ) of the Metallo-β-Lactamase bla VIM Family in a GES-1 Extended-Spectrum-β-Lactamase-Producing Pseudomonas aeruginosa Clinical Isolate in Argentina

Cited by 57 publications

References 8 publications

Sensitive Screening Tests for Suspected Class A Carbapenemase Production in Species of Enterobacteriaceae

Sensitive Screening Tests for Suspected Class A Carbapenemase Production in Species of Enterobacteriaceae

First Case of Human Infection Due to Pseudomonas fulva , an Environmental Bacterium Isolated from Cerebrospinal Fluid

Klebsiella pneumoniaeCarbapenemase–2, Buenos Aires, Argentina

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Novel Variant ( bla _VIM-11 ) of the Metallo-β-Lactamase bla _VIM Family in a GES-1 Extended-Spectrum-β-Lactamase-Producing Pseudomonas aeruginosa Clinical Isolate in Argentina