Novel vacuoles in Toxoplasma

Dooren, Giel G. van; Ralph, Stuart A.

doi:10.1111/j.1365-2958.2010.07197.x

Cited by 2 publications

(2 citation statements)

References 24 publications

(25 reference statements)

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“…In plants, chloroquine-like transporters localize to the plastid, where they appear to play a role in glutathione homeostasis and metabolism, possibly by mediating glutathione export from the organelle (10). In erythrocytic stages of Plasmodium, CRT proteins localize to the digestive vacuole (DV) (5), a lysosome-like organelle that functions primarily in the digestion of hemoglobin (33,34). The DV houses a suite of proteases that digest hemoglobin into its constituent amino acids, which are then utilized for parasite growth (1,33).…”

Section: Discussionmentioning

confidence: 99%

Characterization of the Chloroquine Resistance Transporter Homologue in Toxoplasma gondii

et al. 2014

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Section: Discussionmentioning

confidence: 99%

Characterization of the Chloroquine Resistance Transporter Homologue in Toxoplasma gondii

et al. 2014

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“…Characterization of a vacuolar type pyrophosphatase, TgVP1, and a cathepsin L protease, TgCPL, led to the description of a novel compartment in Toxoplasma gondii [14, 15, 42]. In characterizing the TgVP1 compartment, Miranda and colleagues discovered that it contains other proteins typical of the vacuoles of plants.…”

Section: Discussionmentioning

confidence: 99%

A Toxoplasma gondii protein with homology to intracellular type Na+/H+ exchangers is important for osmoregulation and invasion

Francia

Wicher

Pace

et al. 2011

Experimental Cell Research

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The obligate intracellular parasite Toxoplasma gondii is exposed to a variety of physiological conditions while propagating in an infected organism. The mechanisms by which Toxoplasma overcomes these dramatic changes in its environment are not known. In yeast and plants, ion detoxification and osmotic regulation are controlled by vacuolar compartments. A novel compartment named the plant-like vacuole or vacuolar compartment (PLV/VAC) has recently been described in Toxoplasma gondii, which could potentially protect extracellular tachyzoites against salt and other ionic stresses. Here we report the molecular characterization of the vacuolar type Na+/H+ exchanger in T. gondii, TgNHE3, and its co-localization with the PLV/VAC proton-pyrophosphatase (TgVP1). We have created a TgNHE3 knockout strain, which is more sensitive to hyperosmotic shock and toxic levels of sodium, possesses a higher intracellular Ca2+ concentration [Ca2+]i, and exhibits a reduced host invasion efficiency. The defect in invasion correlates with a measurable reduction in the secretion of the adhesin TgMIC2. Overall, our results suggest that the PLV/VAC, has functions analogous to those of the vacuolar compartments of plants and yeasts, providing the parasite with a mechanism to resist ionic fluctuations, and potentially, regulate protein trafficking.

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Novel vacuoles in Toxoplasma

Cited by 2 publications

References 24 publications

Characterization of the Chloroquine Resistance Transporter Homologue in Toxoplasma gondii

Characterization of the Chloroquine Resistance Transporter Homologue in Toxoplasma gondii

A Toxoplasma gondii protein with homology to intracellular type Na+/H+ exchangers is important for osmoregulation and invasion

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