Novel ultrasensitive immunosensor based on magnetic particles for direct detection of transferrin in blood

Matysiak-Brynda, Edyta; Bystrzejewski, M.; Więckowska, Agnieszka; Grudziński, Ireneusz P.; Nowicka, Anna M.

doi:10.1016/j.snb.2017.04.077

Cited by 10 publications

(4 citation statements)

References 38 publications

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“…Hence, direct electron transfer between the protein and the electrode surface was achieved through the integration of magnetic nanoparticles with monoclonal antibodies, without any need for a mediator molecule. The results showed linearity in the range of 5 × 10 −7 and 5 × 10 −2 g dL −1 , with a limit of detection of 24.0 ± 5.2 ng/dL [ 29 ]. Another label-free immunosensor was designed by Kong et al by utilizing a disposable carbon electrode modified with luminol-reduced gold nanoparticles (Lu-Re-GNP) to detect Tf in serum [ 30 ].…”

Section: Introductionmentioning

confidence: 99%

Clinically Deployable Bioelectronic Sensing Platform for Ultrasensitive Detection of Transferrin in Serum Sample

Kaur

Chittineedi

Bellala³

et al. 2023

Biosensors

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Varying levels of transferrin (Tf) have been associated with different disease conditions and are known to play a crucial role in various malignancies. Regular monitoring of the variations in Tf levels can be useful for managing related diseases, especially for the prognosis of certain cancers. We fabricated an immunosensor based on graphene oxide (GO) nanosheets to indirectly detect Tf levels in cancer patients. The GO nanosheets were deposited onto an indium tin oxide (ITO)-coated glass substrate and annealed at 120 °C to obtain reduced GO (rGO) films, followed by the immobilization of an antibody, anti-Tf. The materials and sensor probe used were systematically characterized by UV–Visible spectroscopy (UV–Vis), X-ray diffraction (XRD), atomic force microscopy (AFM), and Fourier transform infrared spectroscopy (FTIR). Cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS), and differential pulse voltammetry (DPV) were also used for the stepwise sensor probe characterizations and Tf detection in serum samples, respectively. The anti-Tf/rGO/ITO immunosensor DPV output demonstrated an excellent Tf detection capability in the linear range of 0.1 mg mL−1 to 12 mg mL−1 compared to the enzyme-linked immunosorbent assay (ELISA) detection range, with a limit of detection (LOD) of 0.010 ± 0.007 mg mL−1. Furthermore, the results of the fabricated immunosensor were compared with those of the ELISA and autobioanalyzer techniques, showing an outstanding match with < 5% error and demonstrating the immunosensor’s clinical potential.

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Section: Introductionmentioning

confidence: 99%

Clinically Deployable Bioelectronic Sensing Platform for Ultrasensitive Detection of Transferrin in Serum Sample

Kaur

Chittineedi

Bellala³

et al. 2023

Biosensors

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“…For transferrin detection, the routine sensing approach was based on the transferrin antibody immunoassay technique. 25 In this work, a highly selective molecularly imprinted polymer (MIP) anchored on a working electrode surface was demonstrated. The insertion of transferrin into MIP molecular cavities would hinder electron transfer, resulting in a decrease in current signals.…”

Section: ■ Introductionmentioning

confidence: 99%

“…The recognition reaction led to variations in the electric signals as a function of cholesterol concentration. For transferrin detection, the routine sensing approach was based on the transferrin antibody immunoassay technique . In this work, a highly selective molecularly imprinted polymer (MIP) anchored on a working electrode surface was demonstrated.…”

Section: Introductionmentioning

confidence: 99%

An Integrated Sweat Sensor for Synchronous Detection of Multiple Atherosclerosis Biomarkers

Wei,

Zhang,

Chang

et al. 2023

Anal. Chem.

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Atherosclerosis conditions are often assessed in the clinic by measuring blood viscosity, blood flow, and blood lesion levels. In alignment with precision medicine, it is essential to develop convenient and noninvasive approaches for atherosclerosis diagnostics. Herein, an integrated electrochemical sensor was successfully demonstrated for simultaneously detecting cholesterol, transferrin, and K+ in sweat, all biomarker indicators of atherosclerosis. The sensing substrate was based on carbon quantum dots integrated within multiwalled carbon nanotubes, creating a hybrid framework with low electron transfer resistance and highly efficient electron transfer rate, yielding a highly electrochemical active platform for ultrasensitive detection of trace sweat biomarkers. To ensure specificity to corresponding targets, the sensing mechanisms were based on molecular recognition reactions of cholesterol and β-cyclodextrin, transferrin and molecular cavities, and K+ and ion-selective permeation membrane. Moreover, the integrated nonenzymatic sensor exhibited excellent long-term stability. Furthermore, the practical utility of the sensor was successfully demonstrated by the simultaneous detection of three atherosclerosis biomarkers in sweat from volunteers who underwent predesigned daily activities. The sensor shows promise for convenient indexing of atherosclerosis conditions in a noninvasive way.

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“…For examples, antibody-conjugated particles could bind to target molecules in applications such as immunoassays. [20][21][22][23][24][25] Oligonucleotide-conjugated particles are used in the DNA library preparation for high-throughput sequencing. [26,27] Secondly, particles with unique properties, such as color, size and charge, are useful tags to label target molecules or cells.…”

Section: Introductionmentioning

confidence: 99%

Sieve-through vertical flow platform for efficient liquid exchange in particle-based assays

Zhang¹,

Lee²,

Farwin³

et al. 2019

Analytica Chimica Acta

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Novel ultrasensitive immunosensor based on magnetic particles for direct detection of transferrin in blood

Cited by 10 publications

References 38 publications

Clinically Deployable Bioelectronic Sensing Platform for Ultrasensitive Detection of Transferrin in Serum Sample

Clinically Deployable Bioelectronic Sensing Platform for Ultrasensitive Detection of Transferrin in Serum Sample

An Integrated Sweat Sensor for Synchronous Detection of Multiple Atherosclerosis Biomarkers

Sieve-through vertical flow platform for efficient liquid exchange in particle-based assays

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