Novel trypsin–FITC@MOF bioreactor efficiently catalyzes protein digestion

Liu, Wan Ling; Lo, Sheng Han; Singco, Brenda; Yang, Chun; Huang, Hsi Ya; Lin, Chia Her

doi:10.1039/c3tb00257h

Cited by 157 publications

(108 citation statements)

References 37 publications

(25 reference statements)

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“…For [Bmim][OAc] encapsulated MIL‐101(Cr), exemplified by 1.5‐[Bmim][OAc]@MIL‐101, it displayed two steps of weight loss: the first step was related to decomposition of [Bmim][OAc] between 240 and 420°C (Figure a), which was far higher than pure [Bmim] [OAc]. This might arise from the confinement effect of MOF structure, which results in the higher stability of IL in the restricted cavity than on unlimited condition . And the second one was induced by decomposition of terephthalic acid and collapse of the framework in the range of 405–545°C (Figure b).…”

Section: Resultsmentioning

confidence: 99%

Efficient adsorption separation of acetylene and ethylene via supported ionic liquid on metal‐organic framework

et al. 2016

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Ionic liquid (IL) supported metal‐organic framework (MOF) was utilized to efficiently separate acetylene from ethylene. A common IL, 1‐butyl‐3‐methylimidazolium acetate ([Bmim][OAc]), was encapsulated into a hydrothermally stable MOF, namely MIL‐101(Cr). Characterization techniques including FTIR, Powder X‐ray diffraction, BET, and thermal gravimetric analysis were used to confirm successful encapsulation of the IL within MIL‐101(Cr). Adsorption isotherms of acetylene and ethylene in the IL‐encapsulated MOF were tested. From the results, the MOF composite retained a relatively high adsorption capacity. Remarkably, the adsorption selectivity of acetylene/ethylene has dramatically increased from 3.0 to 30 in comparison with the parent MIL‐101(Cr). Furthermore, the potential of industrial practice was examined by breakthrough and regeneration experiments. It not only satisfies the industrial production of removal of low level of acetylene from ethylene, but also is notably stable during the adsorption‐desorption process. The high designability of ILs combined with richness of MOFs’ structures exploits a novel blueprint for gas separation. © 2016 American Institute of Chemical Engineers AIChE J, 63: 2165–2175, 2017

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Section: Resultsmentioning

confidence: 99%

Efficient adsorption separation of acetylene and ethylene via supported ionic liquid on metal‐organic framework

et al. 2016

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“…In addition, cages usually have small windows, which can efficiently encapsulate enzymes, diminishing aggregation, eliminating leaching and optimizing the catalytic environment. Unfortunately, there are very few reported MOFs with enzyme compatible cages, and even fewer can survive in aqueous media 3,11,12 . Thus, MOFs with larger cages and better stability are highly desired for enzyme encapsulation.…”

Section: Resultsmentioning

confidence: 99%

Stable metal-organic frameworks containing single-molecule traps for enzyme encapsulation

et al. 2015

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. However, the engineering of enzymes is severely hampered due to their low operational stability and difficulty of reuse. Here, we develop a series of stable metal-organic frameworks with rationally designed ultra-large mesoporous cages as single-molecule traps (SMTs) for enzyme encapsulation. With a high concentration of mesoporous cages as SMTs, PCN-333(Al) encapsulates three enzymes with record-high loadings and recyclability. Immobilized enzymes that most likely undergo single-enzyme encapsulation (SEE) show smaller K m than free enzymes while maintaining comparable catalytic efficiency. Under harsh conditions, the enzyme in SEE exhibits better performance than free enzyme, showing the effectiveness of SEE in preventing enzyme aggregation or denaturation. With extraordinarily large pore size and excellent chemical stability, PCN-333 may be of interest not only for enzyme encapsulation, but also for entrapment of other nanoscaled functional moieties.

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“…[177] Due to their mutable properties, MOFs show great promise in many potential applications such as catalysis, separation, chemical sensing and drug delivery. [178][179][180][181] MOFs are of interest as promising drug delivery carriers mainly due to their high drug loading capability. However, most studies are focus on delivering small molecular drugs, indeed, many poor solubility and/or stability drugs (e.g., busulfan, Dox, cidofovir, nimesulide, cisplatin, and siRNAs) have been successfully loaded into MOFs which not only protect drugs from biodegradation but also enhance their therapeutic efficacy.…”

Section: Metal-based Nanoparticlesmentioning

confidence: 99%

Rational Design of Nanocarriers for Intracellular Protein Delivery

et al. 2019

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Protein/antibody therapeutics have exhibited the advantages of high specificity and activity even at an extremely low concentration compared to small molecule drugs. However, they are accompanied by unfavorable physicochemical properties such as fragile tertiary structure, large molecular size, and poor penetration of the membrane, and thus the clinical use of protein drugs is hindered by inefficient delivery of proteins into the host cells. To overcome the challenges associated with protein therapeutics and enhance their biopharmaceutical applications, various protein‐loaded nanocarriers with desired functions, such as lipid nanocapsules, polymeric nanoparticles, inorganic nanoparticles, and peptides, are developed. In this review, the different strategies for intracellular delivery of proteins are comprehensively summarized. Their designed routes, mechanisms of action, and potential therapeutics in live cells or in vivo are discussed in detail. Furthermore, the perspective on the new generation of delivery systems toward the emerging area of protein‐based therapeutics is presented as well.

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Novel trypsin–FITC@MOF bioreactor efficiently catalyzes protein digestion

Cited by 157 publications

References 37 publications

Efficient adsorption separation of acetylene and ethylene via supported ionic liquid on metal‐organic framework

Efficient adsorption separation of acetylene and ethylene via supported ionic liquid on metal‐organic framework

Stable metal-organic frameworks containing single-molecule traps for enzyme encapsulation

Rational Design of Nanocarriers for Intracellular Protein Delivery

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