Novel Translational Therapeutic Strategy by Sequencing Primary Liver Cancer Genomes

Glantzounis, Georgios K.; Kyrochristos, Ioannis D; Ziogas, Dimosthenis; Lykoudis, Efstathios G.; Roukos, Dimitrios H

doi:10.2217/fon-2017-0087

Cited by 3 publications

(2 citation statements)

References 19 publications

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“…The majority of patients are diagnosed at an advanced stage, which results in poor survival rates (1). The mortality rate for patients with HCC is second only to that of lung cancer (2–4).…”

Section: Introductionmentioning

confidence: 99%

Forkhead box P3 gene silencing inhibits the expression of chemokines and chemokine receptors associated with cell growth, migration, and apoptosis in hepatocellular carcinoma cells

Zhang

Tian

et al. 2019

Exp Ther Med

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The aberrant expression of forkhead box P3 (FOXP3) leads to the formation of malignant tumors. FOXP3 expression levels are also elevated in hepatocellular carcinoma (HCC). The aim of the present study was to investigate the effects of FOXP3 silencing on cell proliferation, migration, apoptosis and chemokine/chemokine receptor expression in the MHCC-97H HCC cell line. Three FOXP3 short hairpin (sh)RNA constructs were designed: Sh-FOXP3-1-pGreenPuro, sh-FOXP3-2-pGreenPuro, and sh-FOXP3-3-pGreenPuro. MHCC-97H cells were transfected with shRNA-FOXP3, and the mRNA and protein expression levels of C-X-C motif chemokine (CXC) ligand 12 (CXCL12), CXCL11, CXC receptor 4 (CXCR4) and CXCR7 were measured. Cell Counting Kit-8, terminal deoxynucleotidyl-transferase-mediated dUTP nick end labeling and Transwell assays were used to evaluate cell proliferation, apoptosis and migration, respectively. Of the three FOXP3 lentivirus carriers constructed, sh-FOXP3-1 significantly reduced FOXP3 expression levels and was chosen for further experiments. sh-FOXP3-1 inhibited cell proliferation, promoted apoptosis and inhibited cell migration compared with the negative control. The mRNA and protein expression levels of CXCL12, CXCL11, CXCR4 and CXCR7 were decreased significantly in response to FOXP3 silencing. FOXP3 silencing may therefore inhibit cell growth, induce apoptosis and inhibit migration in HCC cells, possibly by impairing the chemokine/chemokine receptor axes.

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Section: Introductionmentioning

confidence: 99%

Forkhead box P3 gene silencing inhibits the expression of chemokines and chemokine receptors associated with cell growth, migration, and apoptosis in hepatocellular carcinoma cells

Zhang

Tian

et al. 2019

Exp Ther Med

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“…The death rate ranks the second in tumor-related deaths. The occurrence and development of liver cancer seriously harm the health and the quality of life of human ( 1 - 3 ). The pathogenesis of liver cancer is very complicated, which involves many factors such as the regulation of the relevant signaling pathways.…”

Section: Introductionmentioning

confidence: 99%

c[RGDyk]-coated liposomes loaded with adriamycin and miR-21 mimics inhibit the growth of hepatoma cell line SMCC-7721 via up-regulating Bax and p53

Wei

You

Zhang

et al. 2019

Transl. Cancer Res.

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Background This study was conducted to investigate the effects of c[RGDyk]-coated liposomes loaded with Adriamycin (nanodrug) and miR-21 mimics on hepatoma cell line SMCC-7721. Methods SMCC-7721 cells were divided into five groups: control (receiving no treatment), nanodrug, miR-21 mimics + nanodrug and miR-21 mimics and empty vector. The concentration and duration of treatments were determined using the MTT assay. Cell apoptosis was detected using flow cytometer. The expression of Bax, Bcl-2 and p53 was measured using qPCR and Western blot analysis. Results MTT showed that the nanodrug inhibited cell proliferation. Nanodrug and miR-21 led to cell arrest at S phase and apoptosis. qPCR showed that cells treated with nanodrug and miR-21 increased the expression of Bax and p53. Western blot analysis indicated that Bcl-2 expression was significantly reduced. Conclusions Our work demonstrates that nanodrug and miR-21 have inhibitory effect on SMCC-7721 cells via up-regulating Bax and p53.

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The role of liver resection in the management of intermediate and advanced stage hepatocellular carcinoma. A systematic review

Glantzounis

Paliouras

Stylianidi

et al. 2018

European Journal of Surgical Oncology

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Novel Translational Therapeutic Strategy by Sequencing Primary Liver Cancer Genomes

Cited by 3 publications

References 19 publications

Forkhead box P3 gene silencing inhibits the expression of chemokines and chemokine receptors associated with cell growth, migration, and apoptosis in hepatocellular carcinoma cells

Forkhead box P3 gene silencing inhibits the expression of chemokines and chemokine receptors associated with cell growth, migration, and apoptosis in hepatocellular carcinoma cells

c[RGDyk]-coated liposomes loaded with adriamycin and miR-21 mimics inhibit the growth of hepatoma cell line SMCC-7721 via up-regulating Bax and p53

The role of liver resection in the management of intermediate and advanced stage hepatocellular carcinoma. A systematic review

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