Novel transcriptional regulation of the GAP promoter in Pichia pastoris towards high expression of heterologous proteins
Xiangna Lin,
Weiqiu Ding,
Shaoyan Zheng
et al.
Abstract:Background
Pichia pastoris (Komagataella phaffii) is a promising production host, but the usage of methanol limits its application in the medicine and food industries.
Results
To improve the constitutive expression of heterologous proteins in P. pastoris, four new potential transcription regulators (Loc1p, Msn2p, Gsm1p, Hot1p) of the glyceraldehyde triphosphate dehydrogenase promoter (pGAP) were revealed in this study by using cellulase E4 as repor… Show more
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