Novel toxicity of native and HD Bacillus thuringiensis strains against to the sugarcane borer Diatraea saccharalis

Rosas-García, Ninfa M.; Pereyra-Alférez, Benito; Árevalo-Niño, Katiushka; ́n-Wong, Luis J. Gal; Morales-Ramos, Lilia H.

doi:10.1023/b:bico.0000034593.45819.e6

Cited by 5 publications

(4 citation statements)

References 16 publications

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“…Strain S76 was about eleven-fold more toxic than HD-1, as estimated by the LC50 values of 13.06 µg/L and of 143.88 µg/L for S76 and HD-1 strains, respectively. A Mexican B. thuringiensis kurstaki strain GM-34 with a much lower LC50 (33.21 µg/mL) (29) has been used in the insecticidal formulation against the sugarcane borer in Mexico (30). Comparison of both strains suggests that the Brazilian isolate is much more efficient than the Mexican bacterium in the control of D. saccharalis.…”

Section: Pcrmentioning

confidence: 99%

“…kurstaki strain HD-1 were carried out with success in the United States (11). More recently, a screening of Mexican B. thuringiensis kurstaki strains against the sugarcane borer resulted in a selection of the strain GM-34 with a LC 50 value of 33.21 µg/mL (29). This bacterium has been used as an active ingredient in the insecticidal formulation prepared to control the sugarcane borer in that country (30).…”

Section: Introductionmentioning

confidence: 99%

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A Brazilian Bacillus thuringiensis strain highly active to sugarcane borer Diatraea saccharalis (Lepidoptera: Crambidae)

Gitahy¹,

De-Souza²,

Monnerat³

et al. 2007

Braz. J. Microbiol.

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The control of the major sugarcane pest, Diatraea saccharalis, is limited by the stem location of the caterpillar. As part of a long-term project towards the development of an alternative and efficient delivery system of Cry proteins to control the sugarcane borer, the current work describes the selection and characterization of a Brazilian B. thuringiensis strain with prominent activity towards D. saccharalis. Strain S76 was eleven-fold more active than the HD-1 Lepidoptera-standard strain, as estimated by the LC50 of 13.06 µg/L and 143.88 µg/ L, respectively. We observed bipiramidal and cuboidal crystals similar to those found in other B. thuringiensis strains with entomopathogenic activity against Lepidoptera and Diptera. In addition, smaller and spherical crystalline inclusions were also observed. The plasmid profile of strain S76 is similar to that of HD-1. PCR amplifications of S76 DNA using cry specific primers confirmed the presence of cry1Aa, cry1Ab, cry1Ac, cry2Aa1, and cry2Ab2, but not cry1Ad, cry2Ac and cry9 type genes. No differences that could explain the superior activity of S76 when compared to HD-1, the Lepidoptera standard strain, were observed. Nevertheless, its higher entomopathogenic activity has pointed this strain S76 as a potential source of cry genes to control sugarcane borer, an important pest that affects sugarcane, a crop that occupies a planted area of about 6 million ha in Brazil.

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Section: Pcrmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

A Brazilian Bacillus thuringiensis strain highly active to sugarcane borer Diatraea saccharalis (Lepidoptera: Crambidae)

Gitahy¹,

De-Souza²,

Monnerat³

et al. 2007

Braz. J. Microbiol.

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“…Among several Bt strains active against the sugarcane borer Diatraea saccharalis Fabricius (Lepidoptera: Pyralidae) obtained from the International Collection from the Department of Microbiology and Immunology, FCB/UANL Mexico, the Bt var. kurstaki strain GM‐34 was chosen as the active ingredient for the formulation owing to its LC 50 of 33.21 µg mL −1 against this pest, as reported previously 32. The strain was maintained on agar slants at 4 °C.…”

Section: Experimental Methodsmentioning

confidence: 99%

Laboratory and field tests of spray‐dried and granular formulations of a Bacillus thuringiensis strain with insecticidal activity against the sugarcane borer

Rosas-García¹

2006

Pest Management Science

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Formulations of Bacillus thuringiensis Berliner (Bt) with insecticidal activity against the sugarcane borer, Diatraea saccharalis Fabricius (Lepidoptera: Pyralidae), were developed and tested under laboratory and field conditions. The formulations were prepared using biodegradable polymers such as modified corn starch as an encapsulating agent, gelatin as an adherent, powdered sugarcane as a feeding stimulant and a Bt var. kurstaki GM-34 strain from a non-sugarcane region as the active ingredient. The spore-crystal complex of this strain was mixed at three different concentrations (30, 70 and 100 g kg(-1)) with the other ingredients. The blends were prepared as spray-dried and granular formulations, and then submitted to laboratory tests with two day old larvae of D. saccharalis and field tests in sugarcane crops with natural sugarcane borer infestation. Spray-dried formulations in laboratory bioassays caused mortality near 100% with all three concentrations, and granular formulations caused mortality around 84%. The field tests showed that spray-dried formulations at 70 and 100 g kg(-1) concentrations were as effective as a commercial bioinsectide (Lepinox), while granular formulations were ineffective.

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“…Twelve Bt strains were evaluated by Rosas García et al [50] at a dosages of 50 and 500 μg of total protein and spores per milliliter against 2-day-old D. saccharalis larvae. The strains used were HD1, HD2, HD9, HD29, HD37, HD59, HD133, HD137, and HD559, as well as the GM7, GM10, and GM34 native strains.…”

Section: Bacteriamentioning

confidence: 99%

Pathogens Associated with Sugarcane Borers,Diatraeaspp. (Lepidoptera: Crambidae): A Review

Hernández-Velázquez

Lina-García

Obregón-Barboza

et al. 2012

International Journal of Zoology

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Novel toxicity of native and HD Bacillus thuringiensis strains against to the sugarcane borer Diatraea saccharalis

Cited by 5 publications

References 16 publications

A Brazilian Bacillus thuringiensis strain highly active to sugarcane borer Diatraea saccharalis (Lepidoptera: Crambidae)

A Brazilian Bacillus thuringiensis strain highly active to sugarcane borer Diatraea saccharalis (Lepidoptera: Crambidae)

Laboratory and field tests of spray‐dried and granular formulations of a Bacillus thuringiensis strain with insecticidal activity against the sugarcane borer

Pathogens Associated with Sugarcane Borers,Diatraeaspp. (Lepidoptera: Crambidae): A Review

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