Novel topical skin hydration agent containing Anadenanthera colubrina polysaccharide‐standardized herbal preparation

Abstract

Background Hydration is an important factor to promote skin barrier function, metabolism, and appearance. In this process, the presence of aquaglyceroporins, envelope and lipid synthesis, and metabolism proteins are essential to provide greater corneocyte cohesion and to form a barrier avoiding transepidermal water loss. Objective We evaluated the effects of a new topical pigment‐free agent containing an Anadenanthera colubrina polysaccharide‐rich dermocosmetic preparation (ACP) on the aquaporin‐3 (AQP‐3), filaggrin (FLG), involucrin (INV), glucocerebrosidase (GBA), and elongation of very‐long‐chain fatty acid (ELOVL) proteins production in skin human fragments, as well as on the transepidermal water loss in a double‐blind placebo‐controlled clinical trial. Methods AQP3, FLG, INV, GBA, and ELOVL3 levels were measured by immunofluorescence analysis in human skin explants. Clinical trial was conducted to evaluate the effects of ACP 1% and ACP 3% on the transepidermal water loss (TEWL). Results Image and statistical analysis showed that ACP 3% significantly increased at 90% the expression of AQP3. Similarly, ACP 3% was able to promote a significant increase of 68% and 51% in FLG and INV, respectively. ACP 3% produced no effects on the GBA and ELOVL3 proteins. Transepidermal water loss was significantly reduced in human volunteers under treatment with ACP 1% and ACP 3%. Conclusion ACP reduced transepidermal water loss in a clinical trial, promoting human skin hydration. These effects were related to modulation of the AQP3, FLG, and INV as evidenced by immunofluorescence assay. This way, A colubrina polysaccharide‐rich phytopharmaceutical preparation is an effective additive product to skin hydration.

Long‐term effects of two 24‐hour moisturizing products on skin barrier structure and function: A biometric and molecular study

“…To the authors' knowledge, the present work is the first molecular assessment on 24‐hour moisturizers; the increased level of AQP3 could explain the improving trend of TEWL and skin hydration even after stopping the product application. Additionally, in an in‐vivo setting, we confirmed the ex vivo report of Caverzan et al, showing that reduced TEWL and promoted human skin hydration are related to modulation of the AQP3 15 . Stimulating the biosynthesis of aquaporin is also associated with an increasing level of CD44, claudin‐1, and filaggrin proteins involved in skin water maintenance 16…”

“…Additionally, in an in-vivo setting, we confirmed the ex vivo report of Caverzan et al, showing that reduced TEWL and promoted human skin hydration are related to modulation of the AQP3. 15 Stimulating the biosynthesis of aquaporin is also associated with an increasing level of CD44, claudin-1, and filaggrin proteins involved in skin water maintenance. 16 Imperata cylindrical is the active ingredients of product A, which has hydrating characteristics due to the presence of natural osmoprotective compounds, including 3-dimethylsulfoniopropionate, potassium, and sugars, in the formulation.…”

Effects of a High-Molecular-Weight Polysaccharides Isolated from Korean Persimmon on the Antioxidant, Anti-Inflammatory, and Antiwrinkle Activity

“…Second, macromolecules, high-molecularweight compounds, which are polymers, composed of multiple monomer units, including carbohydrates (especially polysaccharides) and proteins [19]. In particular, plant polysaccharides have gained great attention in the cosmetic industry owing to their several pharmaceutical effects on human skin [20,21]. Nevertheless, additional studies are required to establish a number of plant-based polysaccharides as functional cosmetic and cosmeceutical ingredients.…”

Clinical study to assess efficacy and safety of Purifying Neem Face Wash in prevention and reduction of acne in healthy adults

“…15 Presently, herbal preparations are used to develop new skincare products with different therapeutic properties that lead to skin hydration and consequently maintain skin water balance. 40 The main limitation of this study was its single-arm observational design without any comparator. Therefore, the effect of the product could not be compared with any other skincare product.…”

“…Hydration is an essential factor to regulate skin barrier function and metabolism, which keeps skin healthy 40 . In acne vulgaris, the skin barrier function has been found to be diminished along with the reduction of the epidermal ceramide level (lowering hydration).…”