Novel tissue units of regional differentiation in the gut epithelium of Drosopbila, as revealed by P-element-mediated detection of enhancer

Murakami, Ryutaro; Shigenaga, Ayako; Matsumoto, Akira; Yamaoka, Ikuo; Tanimura, Teiichi

doi:10.1007/bf00360519

Cited by 40 publications

(47 citation statements)

References 14 publications

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“…Detailed genetic and histological analyses have revealed further regionalization of these midgut regions (see Figure 1a,b for more details) (23, 107a). Such regionalization appears to be a general property of the Drosophila digestive tract given that it has also been observed in the proventriculus and larval midgut and hindgut (94,120,121). The digestive tract is divided into three discrete domains of different developmental origin: foregut, midgut, and hindgut.…”

Section: Compartmentalization Of the Digestive Tractmentioning

confidence: 89%

The Digestive Tract of Drosophila melanogaster

2013

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The digestive tract plays a central role in the digestion and absorption of nutrients. Far from being a passive tube, it provides the first line of defense against pathogens and maintains energy homeostasis by exchanging neuronal and endocrine signals with other organs. Historically neglected, the gut of the fruit fly Drosophila melanogaster has recently come to the forefront of Drosophila research. Areas as diverse as stem cell biology, neurobiology, metabolism, and immunity are benefitting from the ability to study the genetics of development, growth regulation, and physiology in the same organ. In this review, we summarize our knowledge of the Drosophila digestive tract, with an emphasis on the adult midgut and its functional underpinnings. 377

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Section: Compartmentalization Of the Digestive Tractmentioning

confidence: 89%

The Digestive Tract of Drosophila melanogaster

2013

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“…1). The enhancer trap insertion in line 258 (Murakami et al 1994) is expressed at high levels in the gastric caeca and at low levels in the anterior part of the midgut (Fig. 1B).…”

Section: Identification Of Genes That Require Ps1 Integrin Function Fmentioning

confidence: 99%

Uncoupling integrin adhesion and signaling: the beta PS cytoplasmic domain is sufficient to regulate gene expression in the Drosophila embryo

Martín-Bermudo

Brown²

1999

Genes & Development

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Integrin cell surface receptors are ideally suited to coordinate cellular differentiation and tissue assembly during embryogenesis, as they can mediate both signaling and adhesion. We show that integrins regulate gene expression in the intact developing embryo by identifying two genes that require integrin function for their normal expression in Drosophila midgut endodermal cells. We determined the relative roles of integrin adhesion versus signaling in the regulation of these integrin target genes. We find that integrin-mediated adhesion is not required between the endodermal cells and the surrounding visceral mesoderm for integrin target gene expression. In addition, a chimeric protein that lacks integrin-adhesive function, but maintains the ability to signal, can substitute for the endogenous integrin and regulate integrin target genes. This chimera consists of an oligomeric extracellular domain fused to the integrin ␤ PS subunit cytoplasmic domain; a control monomeric extracellular domain fusion does not alter integrin target gene expression. Therefore, oligomerization of the 47-amino-acid ␤ PS intracellular domain is sufficient to initiate a signaling pathway that regulates gene expression in the developing embryo.

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“…The drm-positive region of the midgut corresponds to the domain m13 that was recognized by its characteristic gene expression pattern in larva (Murakami et al, 1994). This region, as is the case of other posterior midgut regions, gives rise to a reabsorptive tissue in larva (Bodensein, 1950).…”

Section: Discussion Drumstick Is Required Cell-non-autonomously For Smentioning

confidence: 99%

“…The following fly stocks are used: Oregon R as wild type for the analyzing normal development, wgPY40 (hypomorphic mutant, Murakami et al, 1994); byn apro (strongest allele, Murakami et al, 1995); srp 2 (amorphic allele; Reuter, 1994) The following GAL4 and UAS strains were used for misexpression experiments: byn-GAL4 (for misexpression in the hindgut primordium); 48Y-GAL4 (for misexpression in the midgut); maternal-GAL4 (ubiquitous); UAS-tll; UAS-wg; UAS-drm; UAS-armS10. All these strains are provided by DGRC, Kyoto Stock Center and Bloomington Stock Center, if not mentioned specifically.…”

Section: Fly Strainsmentioning

confidence: 99%

“…The Drosophila gut consists of three major parts: ectodermal foregut, endodermal midgut and ectodermal hindgut. Each of these gut parts are subsequently subdivided into a number of distinct domains characterized by specific gene expression pattern (Murakami et al, 1994). These features make the Drosophila gut an attractive organ for the study of regional differentiation (Skaer, 1993;Hoch and Pankratz, 1996;Lengyel and Liu, 1998;Murakami et al, 1999;Lengyel and Iwaki, 2002).…”

Section: Introductionmentioning

confidence: 99%

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The drumstick gene acts cell-non-autonomously and triggers specification of the small intestine in the Drosophila hindgut

Uddin¹,

Yano²,

Murakami³

2011

Int. J. Dev. Biol.

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An odd family gene drumstick (drm) encodes a zinc finger protein, and is necessary for the development of the small intestine, an anterior domain of the ectodermal hindgut of Drosophila melanogaster. However, mechanisms that specify the small intestine, as well as gene regulatory pathways leading to transcriptional activation of drm, are still unclear. We found that drm is expressed in two different tissues abutting the anterior end of the hindgut primordium, that is, the posterior-most region of the midgut (endoderm) and basal portion of the Malpighian tubules. A small intestine marker gene, unpaired (upd), begins to be expressed at the anterior-most region of the hindgut primordium that abuts the basal portion of Malpighian tubules, and the upd-positive region expands, resulting in a short tube during stages 11-13. The small intestine develops in both of the mutant embryos, serpent (srp) and Krüppel (Kr), that lack the drm-positive midgut or Malpighian tubules, respectively, while it fails to develop in the Kr srp double-mutant embryos that lack both of the drm-positive tissues. These results demonstrate that drm expressed in the abutting tissues cell-non-autonomously induces development of the small intestine in the hindgut primordium, probably by deploying some extracellular signaling factor. drm expression in the posterior gut region disappears and the small intestine fails to form in tailless (tll) mutant embryos, whereas over-expression of tll causes expansion of drm expression throughout the midgut, inducing a longer small intestine. These results indicate that drm is activated under the control of tll and triggers development of the small intestine cell-non-autonomously through some extracellular signaling.

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Novel tissue units of regional differentiation in the gut epithelium of Drosopbila, as revealed by P-element-mediated detection of enhancer

Cited by 40 publications

References 14 publications

The Digestive Tract of Drosophila melanogaster

The Digestive Tract of Drosophila melanogaster

Uncoupling integrin adhesion and signaling: the beta PS cytoplasmic domain is sufficient to regulate gene expression in the Drosophila embryo

The drumstick gene acts cell-non-autonomously and triggers specification of the small intestine in the Drosophila hindgut

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