Novel Thermoreversible Injectable Hydrogel Formulations Based on Sodium Alginate and Poly(N-Isopropylacrylamide)

β-cyclodextrin (β-CD), with a lipophilic inner cavity and hydrophilic outer surface, interacts with a large variety of non-polar guest molecules to form non-covalent inclusion complexes. Conjugation of β-CD onto biomacromolecules can form physically-crosslinked hydrogel networks upon mixing with a guest molecule. Herein describes the development and characterization of self-healing, thermo-responsive hydrogels, based on host-guest inclusion complexes between alginate-graft-β-CD and Pluronic® F108 (poly(ethylene glycol)-b-poly(propylene glycol)-b-poly(ethylene glycol)). The mechanics, flow characteristics, and thermal response were contingent on the polymer concentrations, and the host-guest molar ratio. Transient and reversible physical crosslinking between host and guest polymers governed self-assembly, allowing flow under shear stress, and facilitating complete recovery of the material properties within a few seconds of unloading. The mechanical properties of the dual-crosslinked, multi-stimuli responsive hydrogels were tuned as high as 30 kPa at body temperature, and are advantageous for biomedical applications such as drug delivery and cell transplantation.

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“…70 Likewise, thermo-responsive alginate hydrogels presented in the literature do not exhibit shear-thinning and self-healing behavior. 71-73 …”

Section: Resultsmentioning

confidence: 99%

Self-Healing and Thermoresponsive Dual-Cross-Linked Alginate Hydrogels Based on Supramolecular Inclusion Complexes

et al. 2015

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“…Among various thermoresponsive polymers, poly( N -isopropylacrylamide) (PNIPAM), a synthetic polymer, has been widely studied as an injectable hydrogel for bone tissue regeneration and drug delivery applications . PNIPAM’s lower critical solution temperature (LCST) is low, with sol-to-gel transition occurring at 32 °C .…”

Section: Introductionmentioning

confidence: 99%

Guar-Based Injectable Thermoresponsive Hydrogel as a Scaffold for Bone Cell Growth and Controlled Drug Delivery

et al. 2018

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In this study, an injectable thermoresponsive hydroxypropyl guar-graft-poly(N-vinylcaprolactam) (HPG-g-PNVCL) copolymer was synthesized by graft polymerization. The reaction parameters such as temperature, time, monomer, and initiator concentrations were varied. In addition, the HPG-g-PNVCL copolymer was modified with nano-hydroxyapatite (n-HA) by in situ covalent cross-linking using divinyl sulfone (DVS) cross-linker to obtain HPG-g-PNVCL/n-HA/DVS composite material. Grafted copolymer and composite materials were characterized using Fourier transform infrared spectroscopy, thermogravimetric analysis, proton nuclear magnetic resonance spectroscopy (1H NMR), and differential scanning calorimetry. The morphology of the grafted copolymer (HPG-g-PNVCL) and the composite (HPG-g-PNVCL/n-HA/DVS) was examined using scanning electron microscopy (SEM), which showed interconnected porous honeycomb-like structures. Using Ultraviolet−visible spectroscopy, low critical solution temperature for HPG-g-PNVCL was observed at 34 °C, which is close to the rheology gel point at 33.5 °C. The thermoreversibility of HPG-g-PNVCL was proved by rheological analysis. The HPG-g-PNVCL hydrogel was employed for slow release of the drug molecule. Ciprofloxacin, a commonly known antibiotic, was used for sustainable release from the HPG-g-PNVCL hydrogel as a function of time at 37 °C because of viscous nature and thermogelation of the copolymer. In vitro cytotoxicity study reveals that the HPG-g-PNVCL thermogelling polymer works as a biocompatible scaffold for osteoblastic cell growth. Additionally, in vitro biomineralization study of HPG-g-PNVCL/n-HA/DVS was conducted using a simulated body fluid, and apatite-like structure formation was observed by SEM.

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“…Nevertheless, PNIPAAm inherently suffers from shortcomings such as its pronounced hydrophobicity above LCST. As a result, hydrogel syneresis is observed, , yielding inadequate mechanical properties and release of entrapped drugs. Moreover, PNIPAAm is not degradable, which poses the problem of its long-term accumulation at the implantation site.…”

Section: Introductionmentioning

confidence: 99%

Inputs of Macromolecular Engineering in the Design of Injectable Hydrogels Based on Synthetic Thermoresponsive Polymers

2020

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Injectable hydrogels have received high attention over the last decades in drug delivery and tissue engineering as they allow minimal invasive procedures. Among them, thermoresponsive-polymer-based hydrogels are considered of high interest owing to their simple, mild, and reproducible preparation conditions, relying only on selfreorganization upon heating. Recent advances in controlled/ living polymerization techniques and chemical conjugation now offer tremendous opportunities for achieving precise engineering and control over such hydrogel properties. In this perspective, we aim at highlighting their recent inputs in the design of thermoresponsive architectures and how they can address key prerequisites of gelation behavior, degradation, biofunctionalization, and controlled drug delivery.

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Novel Thermoreversible Injectable Hydrogel Formulations Based on Sodium Alginate and Poly(N-Isopropylacrylamide)

Cited by 26 publications

References 48 publications

Self-Healing and Thermoresponsive Dual-Cross-Linked Alginate Hydrogels Based on Supramolecular Inclusion Complexes

Self-Healing and Thermoresponsive Dual-Cross-Linked Alginate Hydrogels Based on Supramolecular Inclusion Complexes

Guar-Based Injectable Thermoresponsive Hydrogel as a Scaffold for Bone Cell Growth and Controlled Drug Delivery

Inputs of Macromolecular Engineering in the Design of Injectable Hydrogels Based on Synthetic Thermoresponsive Polymers

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