Novel temperature-responsive polymer brushes with carbohydrate residues facilitate selective adhesion and collection of hepatocytes

Abstract

Temperature-responsive glycopolymer brushes were designed to investigate the effects of grafting architectures of the copolymers on the selective adhesion and collection of hypatocytes. Homo, random and block sequences of N-isopropylacrylamide and 2-lactobionamidoethyl methacrylate were grafted on glass substrates via surface-initiated atom transfer radical polymerization. The galactose/lactose-specific lectin RCA 120 and HepG2 cells were used to test for specific recognition of the polymer brushes containing galactose residues over the lower critical solution temperatures (LCSTs). RCA 120 showed a specific binding to the brush surfaces at 37• C. These brush surfaces also facilitated the adhesion of HepG2 cells at 37• C under nonserum conditions, whereas no adhesion was observed for NIH-3T3 fibroblasts. When the temperature was decreased to 25• C, almost all the HepG2 cells detached from the block copolymer brush, whereas the random copolymer brush did not release the cells. The difference in releasing kinetics of cells from the surfaces with different grafting architectures can be explained by the correlated effects of significant changes in LCST, mobility, hydrophilicity and mechanical properties of the grafted polymer chains. These findings are important for designing 'on-off' cell capture/release substrates for various biomedical applications such as selective cell separation.

Dynamically tunable cell culture platforms for tissue engineering and mechanobiology

“…Functionalization with sugar molecules is also applicable, as these ligands are fascinating candidates for manipulating the interaction between material surfaces and cells. Along these lines, Idota et al developed smart copolymer brushes that are comprised of NIPAAm and 2-lactobionamidoethyl methacrylate (LAMA), which has a galactose residue that binds specifically to the asialoglycoprotein receptors of hepatocytes, and regulates hepatocyte-selective adhesion through temperature alterations [71]. NIH 3T3 fibroblasts did not adhere to the surface of polymer brushes containing LAMA under serum-free conditions, while the surface promoted human hepatocellular carcinoma cell line (HepG2) adhesion at 37°C through specific interactions with functional sugar moieties.…”

Combinational Effect of Cell Adhesion Biomolecules and Their Immobilized Polymer Property to Enhance Cell-Selective Adhesion

“…Platform. The poly(NIPAAm-co-CIPAAm) cell assay platform was prepared using a surface-initiated atom transfer radical polymerization (ATRP) technique [29] (Figure 2). Water-repellent printed slide glasses and regular slide glasses (10 mm × 25 mm) (Matsunami Glass Industry, Tokyo, Japan) were exposed to UV ozone for 10 min for cleaning.…”

“…In this work, we apply the cell assay platform of poly(NIPAAm-co-CIPAAm) [26][27][28][29] to compare cell adhesion performances. Specifically, the changes in the cell adhesion performance and the preference among three different cell types (ECs, FBs, and SMCs), which we refer to as "cell-selective adhesion" (Figure 1), are evaluated.…”

Polymer Brushes with Precise Architectures for Molecular Biorecognition

“…Alternatively, Idota et al [82] have designed temperature-responsive glycopolymer brushes to investigate the effect of the grafting architectures of the copolymer on the selective adhesion and collection of hypatocytes ( Fig. 10.4).…”