Novel techniques for drug loading quantification in mesoporous SBA-15 using chemometric-assisted UV and FT-IR data

Porras, Mauiricio Ariel; Adrover, María Esperanza; Pedernera, Marisa Noemí; Bucalá, Verónica; Gallo, Loreana

doi:10.1016/j.jpba.2022.114830

Cited by 2 publications

(1 citation statement)

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“…Although several publications have reported the non-covalent functionalization of mesoporous silica (SBA-15) with different compounds (drugs) [ 12 , 13 , 14 ], benzofurazans linked to mesoporous silica have not been reported yet. The importance of benzofurazans has been reported in the literature since 1968, being known for their bactericidal, fungicidal, insecticidal activity and, also, inhibitory effects on the biosynthesis of nucleic acids and proteins in various mammals [ 15 ].…”

Section: Introductionmentioning

confidence: 99%

Novel Antitumor Agents Based on Fluorescent Benzofurazan Derivatives and Mesoporous Silica

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Baratoiu-Carpen

et al. 2022

IJMS

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Two novel fluorescent mesoporous silica-based hybrid materials were obtained through the covalent grafting of [4-hydrazinyl-7-nitrobenz-[2,1,3-d]-oxadiazole (NBDH) and N1-(7-nitrobenzo[c][1,2,5]-oxadiazol-4-yl) benzene-1,2-diamine (NBD-PD), respectively, inside the channels of mesoporous silica SBA-15. The presence of fluorescent organic compounds (nitrobenzofurazan derivatives) was confirmed by infrared spectroscopy (IR), X-ray photoelectron spectroscopy (XPS), thermal analysis (TG), and fluorescence spectroscopy. The nitrogen physisorption analysis showed that the nitrobenzofurazan derivatives were distributed uniformly on the internal surface of SBA-15, the immobilization process having a negligible effect on the structure of the support. Their antioxidant activity was studied by measuring the ability to reduce free radicals DPPH (free radical scavenging activity), in order to formulate potential applications of the materials obtained. Cytotoxicity of the newly synthesized materials, SBA-NBDH and SBA-NBD-PD, was evaluated on human B16 melanoma cells. The morphology of these cells, internalization and localization of the investigated materials in melanoma and fibroblast cells were examined through fluorescence imaging. The viability of B16 (3D) spheroids after treatment with SBA-NBDH and SBA-NBD-PD was evaluated using MTS assay. The results showed that both materials induced a selective antiproliferative effect, reducing to various degrees the viability of melanoma cells. The observed effect was enhanced with increasing concentration. SBA-NBD-PD exhibited a higher antitumor effect compared to SBA-NBDH starting with a concentration of 125 µg/mL. In both cases, a significantly more pronounced antiproliferative effect on tumor cells compared to normal cells was observed. The viability of B16 spheroids dropped by 40% after treatment with SBA-NBDH and SBA-NBD-PD at 500 µg/mL concentration, indicating a clear cytotoxic effect of the tested compounds. These results suggest that both newly synthesized biomaterials could be promising antitumor agents for applications in cancer therapy.

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