Novel technique of overlaying a poly-l-lactic acid nanosheet for adhesion prophylaxis and fixation of intraperitoneal onlay polypropylene mesh in a rabbit model

Fujino, Keiichi; Kinoshita, Manabu; Saitoh, Akihiro; Yano, Hidekazu; Nishikawa, Kenichiro; Fujie, Toshinori; Iwaya, Keiichi; Kakihara, Minoru; Takeoka, Shinji; Saitoh, Daizoh; Tanaka, Yuji

doi:10.1007/s00464-011-1745-7

Cited by 24 publications

(30 citation statements)

References 25 publications

(41 reference statements)

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“…Consistent with previous reports (8), when the repaired tissue was placed back inside the abdominal cavity the film did not stick to any opposing tissues and remained adherent strictly to the treatment site.…”

Section: In Vivo Study Observations and Histologysupporting

confidence: 89%

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Repairing Fetal Membranes with a Self-adhesive Ultrathin Polymeric Film: Evaluation in Mid-gestational Rabbit Model

et al. 2014

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Preterm premature rupture of membranes (PPROM) causes 40% of all preterm births, affecting 150,000 women each year in the United States. Prenatal diagnostic procedures and surgical interventions increase incidence of adverse events, leading to iatrogenic membrane rupture after a fetoscopic procedure in 45% of cases. We propose an ultrathin, self-adherent, poly-L-lactic acid patch ("nanofilm") as a reparative wound closure after endoscopic/fetoscopic procedures. These nanofilms are compatible with application in wet conditions and with minimally invasive instrumentation.Ex vivo studies to evaluate the nanofilm were conducted using human chorion-amnion (CA) membranes. A custom-built inflation device was used for mechanical characterization of CA membranes and for assessment of nanofilm adhesion and sealing of membrane defects up to 3 mm in size. These ex vivo tests demonstrated the ability of the nanofilm to seal human CA defects ranging in size from 1 to 3 mm in diameter. In vivo survival studies were conducted in 25 mid-gestational rabbits, defects were created by perforating the uterus and the CA membranes and subsequently using the nanofilm to seal these wounds. These in vivo studies confirmed the successful sealing of defects smaller than 3 mm observed ex vivo.Histological analysis of whole harvested uteri 7 days after surgery showed intact uterine walls in 59% of the nanofilm repaired fetuses, along with increased uterine size and intrauterine development in 63% of the cases. In summary, we have developed an ultrathin, self-adhesive nanofilm for repair of uterine membrane defects.

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Section: In Vivo Study Observations and Histologysupporting

confidence: 89%

“…This same group also demonstrated the efficacy of PLLA sheets as a method for fixing a polypropylene mesh in the peritoneal cavity without inducing visceral adhesions during open surgery (8).…”

Section: Introductionmentioning

confidence: 92%

Repairing Fetal Membranes with a Self-adhesive Ultrathin Polymeric Film: Evaluation in Mid-gestational Rabbit Model

et al. 2014

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“…35,45,46 The composition of these coatings varies from synthetic materials including polymers and metal films, 39,47,48 to biologic materials. Biologic mesh coatings include purified collagen, 40 gelatin, 35 hyaluronic acid, 42–44 chitosan, 38 cellulose, 16,39,43 and fatty acids. 39,43 Several of these biologic coating materials are isolated from ECM but in highly purified forms rather than the complex mixture found in the native ECM microenvironment.…”

Section: Discussionmentioning

confidence: 99%

Polypropylene surgical mesh coated with extracellular matrix mitigates the host foreign body response

Wolf

Carruthers

Dearth

et al. 2013

J. Biomed. Mater. Res.

109

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Surgical mesh devices composed of synthetic materials are commonly used for ventral hernia repair. These materials provide robust mechanical strength and are quickly incorporated into host tissue; factors which contribute to reduced hernia recurrence rates. However, such mesh devices cause a foreign body response with the associated complications of fibrosis and patient discomfort. In contrast, surgical mesh devices composed of naturally occurring extracellular matrix (ECM) are associated with constructive tissue remodeling, but lack the mechanical strength of synthetic materials. A method for applying a porcine dermal ECM hydrogel coating to a polypropylene mesh is described herein with the associated effects upon the host tissue response and biaxial mechanical behavior. Uncoated and ECM coated heavy-weight BARD™ Mesh were compared to the light-weight ULTRAPRO™ and BARD™ Soft Mesh devices in a rat partial thickness abdominal defect overlay model. The ECM coated mesh attenuated the pro-inflammatory response compared to all other devices, with a reduced cell accumulation and fewer foreign body giant cells. The ECM coating degraded by 35 days, and was replaced with loose connective tissue compared to the dense collagenous tissue associated with the uncoated polypropylene mesh device. Biaxial mechanical characterization showed that all of the mesh devices were of similar isotropic stiffness. Upon explantation, the light-weight mesh devices were more compliant than the coated or uncoated heavy-weight devices. The present study shows that an ECM coating alters the default host response to a polypropylene mesh, but not the mechanical properties in an acute in vivo abdominal repair model.

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“…4b). 57 The results evidenced that PLA FsNMs are feasible to induce adhesion prophylaxis in the IPOM, having a beneficial effect as an atraumatic fixation tool. In addition, it was found that nanosheets do not cause inflammatory reaction, which was attributed to the excellent biocompatibility of PLA.…”

Section: Polyester Free-standing Nanomembranesmentioning

confidence: 96%

Insulating and semiconducting polymeric free-standing nanomembranes with biomedical applications

et al. 2015

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In recent decades, polymers have experienced a radical evolution: from being used as inexpensive materials in the manufacturing of simple appliances to be designed as nanostructured devices with important applications in many leading fields, such as biomedicine at the nanoscale. Within this context, polymeric free-standing nanomembranes - self-supported quasi-2D structures with a thickness ranging from similar to 10 to a few hundreds of nanometers and an aspect ratio of size and thickness greater than 10(6) - are emerging as versatile elements for applications as varied as overlapping therapy, burn wound infection treatment, antimicrobial platforms, scaffolds for tissue engineering, drug-loading and delivery systems, biosensors, etc. Although at first, a little over a decade ago, materials for the fabrication of free-standing nanosheets were limited to biopolymers and insulating polymers that were biodegradable, during the last five years the use of electroactive conducting polymers has been attracting much attention because of their extraordinary advantages in the biomedical field. In this context, a systematic review of current research on polymeric free-standing nanomembranes for biomedical applications is presented. Moreover, further discussion on the future developments of some of these exciting areas of study and their principal challenges is presented in the conclusion section.Peer ReviewedPostprint (author's final draft

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Novel technique of overlaying a poly-l-lactic acid nanosheet for adhesion prophylaxis and fixation of intraperitoneal onlay polypropylene mesh in a rabbit model

Abstract: Overlaying of a PLLA nanosheet was effective for adhesion prophylaxis of intraperitoneal mesh. It also may have a possible beneficial effect on fixation of mesh.

Cited by 24 publications

References 25 publications

Repairing Fetal Membranes with a Self-adhesive Ultrathin Polymeric Film: Evaluation in Mid-gestational Rabbit Model

Repairing Fetal Membranes with a Self-adhesive Ultrathin Polymeric Film: Evaluation in Mid-gestational Rabbit Model

Polypropylene surgical mesh coated with extracellular matrix mitigates the host foreign body response

Insulating and semiconducting polymeric free-standing nanomembranes with biomedical applications

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