Novel Synthesis of Fluorescein Isothiocyanate-Based Fluorescent Nanoprobes in Imaging Lung Inflammation

Abstract

We aimed to examine the novel synthesis of fluorescent nanoprobes synthesized in imaging lung inflammation and diseased tissues. All reagents were purchased from commercial suppliers to synthesize the PLGA, PEG, GFP, RFP, rhodamine, and magnetic fluorescent nanoprobes. We performed experiments using human lung cells from the Chinese Academy of Medical Sciences Cell Center. The cells were cultured in a DMEM medium. Confocal microscopy was used to label the cells during imaging. All statistical analyses were performed in GraphPad Prism. There were significant differences in the fluorescent intensities of all nanoprobes. The fluorescence intensity of the iron oxide nanoprobes was significantly higher than all other probes, while Cy5.5 and RFP nanoprobes had significantly higher fluorescence intensity than PEG and FITC. Moreover, we found that GFP has a better quantum yield than RFP, while RFP has longer emission wavelengths than GFP. Fluorescent nanoprobes have shown great potential as a non-invasive and sensitive tool for imaging lung inflammation and diseased tissue.