Novel Supplier of Mesenchymal Stem Cell: Subacromial Bursa

Lhee, S.-H.; Jo, Yunhee; Kim, B.Y.; Nam, Bo Mi; Nemeno, J.G.; Lee, S.; Yang, Wojong; Lee, J.I.

doi:10.1016/j.transproceed.2013.07.052

Cited by 18 publications

(19 citation statements)

References 9 publications

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Section: Discussionmentioning

confidence: 95%

“…Only recently, the BS has been identified by others as a novel source of MSCs within the shoulder, confirming their differentiation potential into the chondrogenic, osteogenic and adipogenic lineage and expression of the surface antigens CD73 and 90 [ 53 ]. Furthermore BS cells were also found to express the surface markers CD29 and platelet-derived growth factor receptor-beta (PDGFRB) [ 53 ], which have not been tested in this study. Additionally, we are able to provide detailed insight into the expression of a series of commonly used surface antigen markers of BS cells compared to BMSCs (Table 1 ) and give insights into the comparative transcriptome characteristics between these two cell types (Fig.…”

Section: Discussionmentioning

confidence: 95%

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Characterization of bursa subacromialis-derived mesenchymal stem cells

et al. 2015

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IntroductionThe bursa subacromialis (BS) provides the gliding mechanism of the shoulder and regenerates itself after surgical removal. Therefore, we explored the presence of mesenchymal stem cells (MSCs) within the human adult BS tissue and characterized the BS cells compared to MSCs from bone marrow (BMSCs) on a molecular level.MethodsBS cells were isolated by collagenase digest from BS tissues derived from patients with degenerative rotator cuff tears, and BMSCs were recovered by adherent culture from bone-marrow of patients with osteoarthritis of the hip. BS cells and BMSCs were compared upon their potential to proliferate and differentiate along chondrogenic, osteogenic and adipogenic lineages under specific culture conditions. Expression profiles of markers associated with mesenchymal phenotypes were comparatively evaluated by flow cytometry, immunohistochemistry, and whole genome array analyses.ResultsBS cells and BMSCs appeared mainly fibroblastic and revealed almost similar surface antigen expression profiles, which was CD44+, CD73+, CD90+, CD105+, CD106+, STRO-1+, CD14−, CD31−, CD34−, CD45−, CD144−. Array analyses revealed 1969 genes upregulated and 1184 genes downregulated in BS cells vs. BMSCs, indicating a high level of transcriptome similarity. After 3 weeks of differentiation culture, BS cells and BMSCs showed a similar strong chondrogenic, adipogenic and osteogenic potential, as shown by histological, immunohistochemical and RT-PCR analyses in contrast to the respective negative controls.ConclusionsOur in vitro characterizations show that BS cells fulfill all characteristics of mesenchymal stem cells, and therefore merit further attention for the development of improved therapies for various shoulder pathologies.

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Section: Discussionmentioning

confidence: 95%

Section: Discussionmentioning

confidence: 95%

Characterization of bursa subacromialis-derived mesenchymal stem cells

et al. 2015

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“… 2 , 7 , 10 , 11 , 12 Additional work has discussed the presence of these cells in subacromial bursa tissue as well. 13 , 14 , 15 , 16 , 17 These studies call into question the routine debridement and disposal of this tissue. 16 , 18 …”

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confidence: 99%

“… 13 , 14 , 15 , 16 , 17 These studies call into question the routine debridement and disposal of this tissue. 16 , 18 …”

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confidence: 99%

Analysis of Time to Form Colony Units for Connective Tissue Progenitor Cells (Stem Cells) Harvested From Concentrated Bone Marrow Aspirate and Subacromial Bursa Tissue in Patients Undergoing Rotator Cuff Repair

Landry

Levy

McCarthy

et al. 2020

Arthroscopy, Sports Medicine, and Rehabilitation

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Purpose To evaluate the time required for colonies to develop from concentrated bone marrow aspirate (cBMA) and subacromial bursal tissue samples. Methods Samples of cBMA and subacromial bursa tissue were harvested from patients undergoing rotator cuff repair surgery between November 2014 and December 2019. Samples were analyzed for time to form colonies and number of colonies formed. The impact of age, sex, and cellularity (cBMA only) was analyzed. Samples were cultured and evaluated daily for colony formation in accordance with the guidelines of the International Society for Cellular Therapy. Demographic factors were analyzed for impact on time to form colonies and number of colonies formed. Results Samples of cBMA were obtained from 92 patients. Subacromial bursa tissue was obtained from 54 patients. For cBMA, older age was associated with more days to form colonies ( P = .003), but sex ( P = .955) and cellularity ( P = .623) were not. For bursa, increased age was associated with longer time to form colonies ( P = .002) but not sex ( P = .804). Conclusions : Increased age (in cBMA and subacromial bursa tissue) and lower initial cellularity (in cBMA) are associated with longer time to form colonies in culture. Clinical Relevance Although connective tissue progenitor cells are widely used in orthopaedic practice, there are few metrics to determine their efficacy. Time to form colonies may serve as an important measurement for determining connective tissue progenitor cell viability for augmentation of rotator cuff repair. Subacromial bursa tissue may represent a viable alternative to cBMA for augmentation of rotator cuff repair, capable of forming colonies expediently in vivo.

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“… 7 The bursa has been found to contain large numbers of MSCs. 8 Even without isolation of specific MSCs, subacromial bursa has been found to have increased differentiation and gene expression potential than concentrated bone marrow aspirate from the proximal humerus. 9 As a result, we have developed a technique to capture the subacromial bursal tissue during subacromial bursectomy and reimplant the tissue on the bursal surface of the rotator cuff tendon after rotator cuff repair.…”

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confidence: 99%

Biologic Augmentation of Arthroscopic Rotator Cuff Repair Using Minced Autologous Subacromial Bursa

Pancholi

Gregory

2020

Arthroscopy Techniques

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Failure of rotator cuff repair surgery can be attributed to a variety of factors, including insufficient biologic environment to support healing. The subacromial bursal tissue has been shown to have a reservoir of mesenchymal stem cells and is a potential source for biologic augmentation during rotator cuff repair. We have developed a technique to capture the subacromial bursal tissue during subacromial bursectomy and then reimplant the tissue on the bursal surface of the rotator cuff tendon after rotator cuff repair. Our goal is to describe our technique of subacromial tissue collection and reimplantation that obviates the need of suturing a whole sleeve of bursal tissue while improving cell yield for rotator cuff healing.

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Novel Supplier of Mesenchymal Stem Cell: Subacromial Bursa

Cited by 18 publications

References 9 publications

Characterization of bursa subacromialis-derived mesenchymal stem cells

Characterization of bursa subacromialis-derived mesenchymal stem cells

Analysis of Time to Form Colony Units for Connective Tissue Progenitor Cells (Stem Cells) Harvested From Concentrated Bone Marrow Aspirate and Subacromial Bursa Tissue in Patients Undergoing Rotator Cuff Repair

Biologic Augmentation of Arthroscopic Rotator Cuff Repair Using Minced Autologous Subacromial Bursa

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