Novel structures in marginal and intermediate cells presumably relate to functions of apical versus basal strial strata

Spicer, Samuel S.; Schulte, Bradley A.

doi:10.1016/j.heares.2004.09.006

Cited by 27 publications

(31 citation statements)

References 50 publications

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“…These results strongly suggest that the ␣ 1 -subunit is localized at either the basolateral membrane of marginal cells or the apical site of intermediate cells. The resolution of confocal microscopic analysis was, however, insufficient to precisely determine the localization of the ␣ 1 -subunit, because the basolateral membrane of marginal cells and the apical membrane of intermediate cells are associated in close vicinity of ϳ150 -200 Å and are prominently invaginated (28,72). In spiral ganglions, we detected moderate labeling of the ␣ 1 -subunit in the neurons but not in satellite cells (Fig.…”

Section: Fig 2 Expression and Distribution Of Hmentioning

confidence: 87%

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Gastric type H⁺,K⁺-ATPase in the cochlear lateral wall is critically involved in formation of the endocochlear potential

Shibata

Hibino²,

Doi³

et al. 2006

American Journal of Physiology-Cell Physiology

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Cochlear endolymph has a highly positive potential of approximately +80 mV known as the endocochlear potential (EP). The EP is essential for hearing and is maintained by K+ circulation from perilymph to endolymph through the cochlear lateral wall. Various K+ transport apparatuses such as the Na+,K+-ATPase, the Na+-K+-2Cl− cotransporter, and the K+ channels Kir4.1 and KCNQ1/KCNE1 are expressed in the lateral wall and are known to play indispensable roles in cochlear K+ circulation. The gastric type of the H+,K+-ATPase was also shown to be expressed in the cochlear lateral wall (Lecain E, Robert JC, Thomas A, and Tran Ba Huy P. Hear Res 149: 147–154, 2000), but its functional role has not been well studied. In this study we examined the precise localization of H+,K+-ATPase in the cochlea and its involvement in formation of EP. RT-PCR analysis showed that the cochlea expressed mRNAs of gastric α1-, but not colonic α2-, and β-subunits of H+,K+-ATPase. Immunolabeling of an antibody specific to the α1 subunit was detected in type II, IV, and V fibrocytes distributed in the spiral ligament of the lateral wall and in the spiral limbus. Strong immunoreactivity was also found in the stria vascularis. Immunoelectron microscopic examination exhibited that the H+,K+-ATPase was localized exclusively at the basolateral site of strial marginal cells. Application of Sch-28080, a specific inhibitor of gastric H+,K+-ATPase, to the spiral ligament as well as to the stria vascularis caused prominent reduction of EP. These results may imply that the H+,K+-ATPase in the cochlear lateral wall is crucial for K+ circulation and thus plays a critical role in generation of EP.

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Section: Fig 2 Expression and Distribution Of Hmentioning

confidence: 87%

“…Morphologically, marginal cells can be identified with their highly convoluted and infolded basolateral membrane (72). We also visualized barttin immunoreactivity as another hallmark for marginal cells (15).…”

Section: Fig 2 Expression and Distribution Of Hmentioning

confidence: 92%

Gastric type H⁺,K⁺-ATPase in the cochlear lateral wall is critically involved in formation of the endocochlear potential

Shibata

Hibino²,

Doi³

et al. 2006

American Journal of Physiology-Cell Physiology

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“…Because marginal cell density was of special interest among strial cell types, marginal cells were counted in the lower base, upper base, and lower apex, while other cell types were quantified only in the upper base. Spicer and Schulte (2005b). Unconnected capillary profiles were counted over the entire strial profile in the lower base, upper base, and lower apex (Fig.…”

Section: Stria Vascularismentioning

confidence: 99%

Divergent Aging Characteristics in CBA/J and CBA/CaJ Mouse Cochleae

Ohlemiller

Dahl

Gagnon

2010

JARO

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Two inbred mouse strains, CBA/J and CBA/CaJ, have been used nearly interchangeably as 'good hearing' standards for research in hearing and deafness. We recently reported, however, that these two strains diverge after 1 year of age, such that CBA/CaJ mice show more rapid elevation of compound action potential (CAP) thresholds at high frequencies (Ohlemiller, Brain Res. 1277: 70-83, 2009). One contributor is progressive decline in endocochlear potential (EP) that appears only in CBA/CaJ. Here, we explore the cellular bases of threshold and EP disparities in old CBA/J and CBA/CaJ mice. Among the major findings, both strains exhibit a characteristic age (∼18 months in CBA/J and 24 months in CBA/CaJ) when females overtake males in sensitivity decline. Strain differences in progression of hearing loss are not due to greater hair cell loss in CBA/ CaJ, but instead appear to reflect greater neuronal loss, plus more pronounced changes in the lateral wall, leading to EP decline. While both male and female CBA/CaJ show these pathologies, they are more pronounced in females. A novel feature that differed sharply by strain was moderate loss of outer sulcus cells (or 'root' cells) in spiral ligament of the upper basal turn in old CBA/CaJ mice, giving rise to deep indentations and void spaces in the ligament. We conclude that CBA/CaJ mice differ both quantitatively and qualitatively from CBA/J in age-related cochlear pathology, and model different types of presbycusis.

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“…Marginal cells, intermediate cells, and basal cells were counted in an 80 μm linear segment of stria, centered at the midpoint. No attempt was made to distinguish between lower and upper level intermediate cells, a distinction recently made by Schulte and Spicer (Spicer and Schulte 2005b). Ligament thickness was measured on an axis co-linear with the strial midline.…”

Section: Assessment Of Permanent Noise Injury (8 Week After Noise)mentioning

confidence: 99%

Different Cellular and Genetic Basis of Noise-Related Endocochlear Potential Reduction in CBA/J and BALB/cJ Mice

Ohlemiller

Rosen

Rellinger

et al. 2010

JARO

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The acute and permanent effects of noise exposure on the endocochlear potential (EP) and cochlear lateral wall were evaluated in BALB/cJ (BALB) inbred mice, and compared with CBA/J (CBA) and C57BL/6 (B6) mice. Two-hour exposure to broadband noise (4-45 kHz) at 110 dB SPL leads to a~50 mV reduction in the EP in BALB and CBA, but not B6. EP reduction in BALB and CBA is reliably associated with characteristic acute cellular pathology in stria vascularis and spiral ligament. By 8 weeks after exposure, the EP in CBA mice has returned to normal. In BALBs, however, the EP remains depressed by an average~10 mV, so that permanent EP reduction contributes to permanent threshold shifts in these mice. We recently showed that the CBA noise phenotype in part reflects the influence of a large effect quantitative trait locus on Chr. 18, termed Nirep (Ohlemiller et al., Hear Res 260:47-53, 2010b). While CBA "EP susceptibility" alleles are dominant to those in B6, examination of (B6×BALB) F1 hybrid mice and (F1×BALB) N2 backcross mice revealed that noise-related EP reduction and associated cell pathology in BALBs are inherited in an autosomal recessive manner, and are dependent on multiple genes. Moreover, while N2 mice formed from B6 and CBA retain strong correspondence between acute EP reduction, ligament pathology, and strial pathology, N2s formed from B6 and BALB include subsets that dissociate pathology of ligament and stria. We conclude that the genes and cascades that govern the very similar EP susceptibility phenotypes in BALB and CBA mice need not be the same. BALBs appear to carry alleles that promote more pronounced long term effects of noise on the lateral wall. Separate loci in BALBs may preferentially impact stria versus ligament.

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Novel structures in marginal and intermediate cells presumably relate to functions of apical versus basal strial strata

Cited by 27 publications

References 50 publications

Gastric type H⁺,K⁺-ATPase in the cochlear lateral wall is critically involved in formation of the endocochlear potential

Gastric type H⁺,K⁺-ATPase in the cochlear lateral wall is critically involved in formation of the endocochlear potential

Divergent Aging Characteristics in CBA/J and CBA/CaJ Mouse Cochleae

Different Cellular and Genetic Basis of Noise-Related Endocochlear Potential Reduction in CBA/J and BALB/cJ Mice

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Novel structures in marginal and intermediate cells presumably relate to functions of apical versus basal strial strata

Cited by 27 publications

References 50 publications

Gastric type H+,K+-ATPase in the cochlear lateral wall is critically involved in formation of the endocochlear potential

Gastric type H+,K+-ATPase in the cochlear lateral wall is critically involved in formation of the endocochlear potential

Divergent Aging Characteristics in CBA/J and CBA/CaJ Mouse Cochleae

Different Cellular and Genetic Basis of Noise-Related Endocochlear Potential Reduction in CBA/J and BALB/cJ Mice

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Product

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Gastric type H⁺,K⁺-ATPase in the cochlear lateral wall is critically involved in formation of the endocochlear potential

Gastric type H⁺,K⁺-ATPase in the cochlear lateral wall is critically involved in formation of the endocochlear potential