Novel strategies in tendon and ligament tissue engineering: Advanced biomaterials and regeneration motifs

Kuo, Catherine K.; Marturano, Joseph E.; Tuan, Rocky S.

doi:10.1186/1758-2555-2-20

Cited by 135 publications

(177 citation statements)

References 89 publications

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“…For a detailed review of biomaterials and scaffold designs, and growth factors currently utilized in engineering ligament tissue, refer to the following reviews. [20][21][22][23] In the approach taken here, isolated bone marrow stromal cells (BMSCs) are differentiated along osteogenic and fibrogenic pathways and in doing so, generate their own extra-cellular matrices. These matrices are then manipulated by the cells via contraction to form the constructs without artificial scaffolds.…”

Section: Introductionmentioning

confidence: 99%

Three-Dimensional Engineered Bone–Ligament–Bone Constructs for Anterior Cruciate Ligament Replacement

Smietana

Kostrominova

et al. 2012

Tissue Engineering Part A

114

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The anterior cruciate ligament (ACL), a major stabilizer of the knee, is commonly injured. Because of its intrinsic poor healing ability, a torn ACL is usually reconstructed by a graft. We developed a multi-phasic, or boneligament-bone, tissue-engineered construct for ACL grafts using bone marrow stromal cells and sheep as a model system. After 6 months in vivo, the constructs increased in cross section and exhibited a well-organized microstructure, native bone integration, a functional enthesis, vascularization, innervation, increased collagen content, and structural alignment. The constructs increased in stiffness to 52% of the tangent modulus and 95% of the geometric stiffness of native ACL. The viscoelastic response of the explants was virtually indistinguishable from that of adult ACL. These results suggest that our constructs after implantation can obtain physiologically relevant structural and functional characteristics comparable to those of adult ACL. They present a viable option for ACL replacement.

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Section: Introductionmentioning

confidence: 99%

Three-Dimensional Engineered Bone–Ligament–Bone Constructs for Anterior Cruciate Ligament Replacement

Smietana

Kostrominova

et al. 2012

Tissue Engineering Part A

114

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“…Mechanical stimuli, when provided in a proper manner, induce the growth and better alignment of cells, improving the quality of ECM 5,6 , however, the frequency as well as the intensity of these stimuli are not yet well known 2 .…”

Section: Introductionmentioning

confidence: 99%

“…However, they experience some of the highest mechanical loads of the body. When these loads exceed a critical threshold, injuries may occur, resulting in morphofunctional changes and disorder of movements 5 . Ligaments are functional (effective) under stress or stretch, however, without compression or functionality when shortened beyond their rest threshold 2 .…”

Section: Introductionmentioning

confidence: 99%

Avaliação dos ligamentos longitudinais da coluna de ratos Wistar em modelo experimental da terapia Suit

et al. 2016

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| Ligaments adapt according to the intensity of physical activity and mechanical load to which they are subjected. In the last decade there have been methods and protocols in the field of infant neurofunctional physiotherapy, which have the term "suit" in common, to characterize the existence of suits with adjustable elastic bands and the possibility of applying load on the human skeleton. Since the mechanical load can produce fibrocartilaginous changes on the ligaments and also that no studies evaluating the effect of suit therapy on ligaments of the spine were found, research with experimental methods of load are justified. The aim of this study was to analyze thickness and morphology of longitudinal ligaments of the spine of Wistar rats when subjected to mechanical load by vertebral compression. Thirty animals were separated into five groups (G1 -control; G2 -simulation of the use of suit; G3, G4, and G5 -maintenance of the suit). The suit experimental model, in G4 and G5, were adapted weights or elastic bands arranged in "X" for 50% of spinal overload of the weight of the animal, who remained with the suit for 40 hours over four weeks of experiment, five days a week.There were no significant differences for thickness, and morphological changes of longitudinal ligaments were also not observed. We concluded that there were no changes in longitudinal ligaments of the spine in animals subjected to the experimental model of suit therapy. RESUMEN | Pueden adaptarse los ligamentos a la intensidad de la actividad física y a la carga mecánica sometida a ellos. En la última década, han surgido métodos y protocolos en el área de fisioterapia neurofuncional infantil que tienen en común el término "suit" para caracterizar la existencia de ropas con bandas elásticas ajustables y la posibilidad de aplicación de cargas en el esqueleto humano. Debido a que la carga mecánica puede producir alteraciones fibrocartilaginosas en los ligamentos y que no han sido encontrados estudios que evaluaron el efecto de la terapia suit en los ligamentos de la columna vertebral, las investigaciones con métodos experimentales de carga son necesarias. Este estudio tiene el propósito de evaluar las espesuras y la morfología de los ligamentos longitudinales de la columna vertebral de ratas Wistar cuando sometida a carga mecánica por compresión vertebral. Se dividieron treinta ratas en cinco grupos (G1 -grupo control; G2 -simulación de la utilización del suit; G3, G4, G5 -mantenimiento de la ropa).Desde el modelo experimental del suit, en el G4 y G5 se adaptaron pesos o elásticos puestos en "X" para la sobrecarga vertebral de 50% del peso de los animales, los cuales permanecieron con la ropa durante cuarenta horas, al largo de cuatro semanas de experimento, en cinco días semanales. No fueron observadas diferencias significativas para la espesura, tampoco cambios morfológicos en los ligamentos longitudinales. Se concluye que no se observaron alteraciones en los ligamentos longitudinales de la columna vertebral de los animales sometidos al ...

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“…16,20 Several approaches used the slow release of stimulatory growth factors (GFs) attached to the scaffolds. 14,17,21 All these modifications require many testing experiments under highly standardized conditions. The presence of stimulatory GFs or natural ligament ECM is especially advantageous if precursor cells are used to produce a tissue engineered neotendon or-ligament.…”

mentioning

confidence: 99%

“…6,7 Therefore, functionalization of synthetic polymers is demanded. 12,13,17 Biomaterial functionalization should increase cell adherence, survival, spreading and migration, proliferation or differentiation and at least ligamentogenesis. It could also be applied to adapt biomechanical properties.…”

mentioning

confidence: 99%

Do There Exist Innovative Perspectives in Tissue Engineering-Based Ligament Reconstruction?

Tanzil¹

2017

ATROA

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Ligament reconstruction remains challenging, since ligaments represent heavily loaded tissues with high extracellular matrix (ECM) content, very low cell numbers, limited blood supply and as a consequence, restricted repair capacity. To circumvent limited auto graft availability and donor site morbidity, tissue engineered biological grafts could present benefit for ligament reconstruction in future. Valuable tissue engineered approaches can be developed based on small-scale in vitro models.This mini review was prepared to draw attention to some experimental key problems and to illustrate approaches to establish tissue engineered ligament substitutes including appropriate scaffolds, functionalization of them, applicable cell sources, three-dimensional (3D) cell culturing and seeding strategies. It opens also the view on novel perspectives, which could move ligament tissue engineering forward such as utilization of various natural allogenic and xengenic ligament-derived ECMs and first attempts in ligament bioprinting.

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Novel strategies in tendon and ligament tissue engineering: Advanced biomaterials and regeneration motifs

Cited by 135 publications

References 89 publications

Three-Dimensional Engineered Bone–Ligament–Bone Constructs for Anterior Cruciate Ligament Replacement

Three-Dimensional Engineered Bone–Ligament–Bone Constructs for Anterior Cruciate Ligament Replacement

Avaliação dos ligamentos longitudinais da coluna de ratos Wistar em modelo experimental da terapia Suit

Do There Exist Innovative Perspectives in Tissue Engineering-Based Ligament Reconstruction?

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