Novel squalene-producing thraustochytrids found in mangrove water

Otagiri, Masato; Khalid, Ammara; Moriya, Shigeharu; Osada, Hiroyuki; Takahashi, Shunji

doi:10.1080/09168451.2017.1359485

Cited by 16 publications

(7 citation statements)

References 20 publications

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“…MAN70 showed a higher level of squalene production than MAN65, accumulating up to 13 mg/g DW. This accumulation profile is not very different from data published for 132 analyzed isolates, which mostly showed squalene yields between 7.5 and 33 mg/g DW [17,20]. The highest concentrations of squalene were reported in Aurantiochytrium sp.…”

Section: Intracellular Production Of Lipids Protein Carbohydrates contrasting

confidence: 44%

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The Nutritional and Pharmacological Potential of New Australian Thraustochytrids Isolated from Mangrove Sediments

et al. 2020

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Mangrove sediments represent unique microbial ecosystems that act as a buffer zone, biogeochemically recycling marine waste into nutrient-rich depositions for marine and terrestrial species. Marine unicellular protists, thraustochytrids, colonizing mangrove sediments have received attention due to their ability to produce large amounts of long-chain ω3-polyunsaturated fatty acids. This paper represents a comprehensive study of two new thraustochytrids for their production of valuable biomolecules in biomass, de-oiled cakes, supernatants, extracellular polysaccharide matrixes, and recovered oil bodies. Extracted lipids (up to 40% of DW) rich in polyunsaturated fatty acids (up to 80% of total fatty acids) were mainly represented by docosahexaenoic acid (75% of polyunsaturated fatty acids). Cells also showed accumulation of squalene (up to 13 mg/g DW) and carotenoids (up to 72 µg/g DW represented by astaxanthin, canthaxanthin, echinenone, and β-carotene). Both strains showed a high concentration of protein in biomass (29% DW) and supernatants (2.7 g/L) as part of extracellular polysaccharide matrixes. Alkalinization of collected biomass represents a new and easy way to recover lipid-rich oil bodies in the form of an aqueous emulsion. The ability to produce added-value molecules makes thraustochytrids an important alternative to microalgae and plants dominating in the food, pharmacological, nutraceutical, and cosmetics industries.

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Section: Intracellular Production Of Lipids Protein Carbohydrates contrasting

confidence: 44%

“…Aurantiochytrium species showed the highest concentration of squalene among thraustochytrids, accumulating up to 318 mg/g DW [1,16]. Other representatives of thraustochytrids showed a lower level of squalene production, 0.15-84 g/kg DW [17][18][19][20].…”

Section: Introductionmentioning

confidence: 99%

The Nutritional and Pharmacological Potential of New Australian Thraustochytrids Isolated from Mangrove Sediments

et al. 2020

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“…For example, A. limacinum displays five times more squalene than its close relative H. fermentalgiana [6]. However, a recent study identified new highly producing strains, forming a distinct clade on a phylogenetic tree and branching out of the Aurantiochytrium cluster [120]. These new thraustochytrid members are potential novel candidates for the commercial production of squalene.…”

Section: Carotenoids Sterols and Squalenementioning

confidence: 99%

The lipid metabolism in thraustochytrids

Morabito

Bournaud

Maës

et al. 2019

Progress in Lipid Research

130

100

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Thraustochytrids are unicellular heterotrophic marine protists of the Stramenopile group, often considered as non-photosynthetic microalgae. They have been isolated from a wide range of habitats including deep sea, but are mostly present in waters rich in sediments and organic materials. They are abundant in mangrove forests where they are major colonizers, feeding on decaying leaves and initiating the mangrove food web. Discovered 80 years ago, they have recently attracted considerable attention due to their biotechnological potential. This interest arises from their fast growth, their specific lipid metabolism and the improvement of the genetic tools and transformation techniques. These organisms are particularly rich in ω3-docosahexaenoic acid (DHA), an 'essential' fatty acid poorly encountered in land plants and animals but required for human health. To produce their DHA they use a complex system different from the classical fatty acid synthase system. They are also a potential source of squalene and carotenoids. Here we review our current knowledge about the life cycle, ecophysiology, and metabolism of these organisms, with a particular focus on lipid dynamics. We describe the different pathways involved in lipid and fatty acid syntheses, emphasizing their specificity, and we report on the recent efforts aimed to engineer their lipid metabolism.

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“…14 novel squalene producing thraustochytrid strains were isolated and identi ed. The amount of produced squalene was determined between 7.54 to 13.9 g/g CDW [44].…”

Section: Phylogenetic Analysis Of Strain Dr37mentioning

confidence: 99%

Rhodosporidium Sp. DR37: A Novel Strain For Production of Squalene in Optimized Cultivation Conditions

Shakeri

Khoshbasirat

Maleki³

2021

Preprint

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Background: Rhodosporidium strain, a well-known oleaginous yeast, has been widely used as a platform for lipid and carotenoid production. However, the production of squalene for application in lipid-based biofuels is not reported in this strain. Here, we isolated and identified newly strain of Rhodosporidium sp. DR37 and investigated its potential for production of squalene under various cultivation conditions.Results: In the present study, Rhodosporidium sp. DR37 was isolated from mangrove ecosystem and its potential for squalene production was assessed. When Rhodosporidium sp. DR37 was cultivated on non-optimized medium (20 g/L glucose, 5 g/L peptone, 5 g/L YE, 15 g/L agar, seawater (50% v/v), pH 7, 30 °C), 64 mg/L of squalene was produced. Significantly, use of optimized medium (20 g/L sucrose, 5 g/L peptone, seawater (20 % v/v), pH 7, 25 °C) allowed highest squalene accumulation in Rhodosporidium sp. DR37 (619 mg/L). The maximum squalene content was obtained as 21.6% of total lipid in comparison to the non-optimized medium (13.9% of total lipid).Conclusions: This study is the first report to employ marine oleaginous Rhodosporidium sp. DR37 for accumulation of squalene in optimized medium. Our findings provide the potential of Rhodosporidium sp. DR37 for production of squalene as well as lipid and carotenoids for biofuel applications in large scale.

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Novel squalene-producing thraustochytrids found in mangrove water

Cited by 16 publications

References 20 publications

The Nutritional and Pharmacological Potential of New Australian Thraustochytrids Isolated from Mangrove Sediments

The Nutritional and Pharmacological Potential of New Australian Thraustochytrids Isolated from Mangrove Sediments

The lipid metabolism in thraustochytrids

Rhodosporidium Sp. DR37: A Novel Strain For Production of Squalene in Optimized Cultivation Conditions

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