Novel spectrophotometric technique for rapid determination of extractable PHA using Sudan black dye

Porras, Mauiricio Ariel; Villar, Marcelo A.; Cubitto, María Amelia

doi:10.1016/j.jbiotec.2017.06.012

Cited by 15 publications

(12 citation statements)

References 18 publications

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“…As it can be seen, the temperature range assayed in these experiences did not signi cantly affect the PHB productivity (Figure 3b,c), despite these values are within the optimal growing temperature range for this strain (Porras et al 2017 As it was aforementioned, vinasse sample employed in the optimization of the media composition was V 2017 , meanwhile for the optimization of growing conditions it was employed V 2018 . The variability in physicochemical properties of both vinasse samples led to signi cant differences in PHB productivity.…”

Section: Optimization Of Culture Media Composition and Fermentation Conditionsmentioning

confidence: 63%

“…In this study, it was employed Bacillus megaterium to synthetize biopolymers via microbial fermentation. According to López et al (2012) and Porras et al(2017), this strain is capable of producing PHB and PHA copolymers, respectively. To optimize culture media composition, it was carried out 15 experiments given by the Box-Behnken experimental design and obtained PHB productivity (P) values are included in Table 2.…”

Section: Optimization Of Culture Media Composition and Fermentation Conditionsmentioning

confidence: 99%

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Vinasse: From A Residue To A High Added Value Biopolymer

Trapé¹,

López²,

Villar³

2021

Preprint

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This work aimed to study the feasibility to use vinasse for polyhydroxybutyrate (PHB) production by Bacillus megaterium. To optimize the culture medium, a Box Behnken design was employed considering carbon (C), nitrogen (N), and phosphorus (Ph) concentrations as independent variables and PHB productivity as the response variable. The productivity decreased when C or N were increased, probably due to the presence of phenolic compounds and the limitation of N for the production of PHB by Bacillus sp. bacteria. An additional experimental design to optimize the C/N ratio and growing conditions (fermentation time and temperature) was carried out. Fermentation time had a statistically significant effect on PHB productivity reaching 10.6 mg/L h. On the other hand, the variability in physicochemical properties of vinasse samples led to significant differences in PHB productivity. Lower productivity values were obtained when vinasse had DBO high values. Therefore, biopolymers production from vinasse is a feasible alternative to valorize this bioethanol by-product.

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Section: Optimization Of Culture Media Composition and Fermentation Conditionsmentioning

confidence: 63%

Section: Optimization Of Culture Media Composition and Fermentation Conditionsmentioning

confidence: 99%

Vinasse: From A Residue To A High Added Value Biopolymer

Trapé¹,

López²,

Villar³

2021

Preprint

Self Cite

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“…Sudan black B (C29H24N6) is a diazo fat-soluble dye [58]. PHAs are observed as black granules with Sudan black B by bright-field microscopy [59].…”

Section: Discussionmentioning

confidence: 99%

Haloarchaea as Cell Factories to Produce Bioplastics

et al. 2021

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Plastic pollution is a worldwide concern causing the death of animals (mainly aquatic fauna) and environmental deterioration. Plastic recycling is, in most cases, difficult or even impossible. For this reason, new research lines are emerging to identify highly biodegradable bioplastics or plastic formulations that are more environmentally friendly than current ones. In this context, microbes, capable of synthesizing bioplastics, were revealed to be good models to design strategies in which microorganisms can be used as cell factories. Recently, special interest has been paid to haloarchaea due to the capability of some species to produce significant concentrations of polyhydroxyalkanoate (PHA), polyhydroxybutyrate (PHB), and polyhydroxyvalerate (PHV) when growing under a specific nutritional status. The growth of those microorganisms at the pilot or industrial scale offers several advantages compared to that of other microbes that are bioplastic producers. This review summarizes the state of the art of bioplastic production and the most recent findings regarding the production of bioplastics by halophilic microorganisms with special emphasis on haloarchaea. Some protocols to produce/analyze bioplastics are highlighted here to shed light on the potential use of haloarchaea at the industrial scale to produce valuable products, thus minimizing environmental pollution by plastics made from petroleum.

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“…[ 12 ] A novel spectrophotometric technique to quantify PHA using Sudan black dye with maximum absorbance value at around 660–680 nm was developed by Porras et al. [ 13 ]…”

Section: Identification Quantification and Characterization Of Phamentioning

confidence: 99%

“…[ 8 ] For its quantification and analysis, many methods such as GC‐MS, NMR, FTIR, and spectrophotometry have been used, but there is no simple or unified quantitative method for the same. [ 12 ] Spectrophotometric method using Sudan black dye has been used as a proxy for PHA analysis in multiple studies [ 13 ] as a GC‐based quantification approach is not convenient for day‐to‐day analysis.…”

Section: Introductionmentioning

confidence: 99%

Process optimization, metabolic engineering interventions and commercialization of microbial polyhydroxyalkanoates production – A state‐of‐the art review

Lhamo

Behera

Mahanty

2021

Biotechnology Journal

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Background Microbial polyhydroxyalkanoates (PHAs) produced using renewable resources could be the best alternative for conventional plastics. Despite their incredible potential, commercial production of PHAs remains very low. Nevertheless, sincere attempts have been made by researchers to improve the yield and economic viability of PHA production by utilizing low‐cost agricultural or industrial wastes. In this context, the use of efficient microbial culture or consortia, adoption of experimental design to trace ideal growth conditions, nutritional requirements, and intervention of metabolic engineering tools have gained significant attention. Purpose and scope This review has been structured to highlight the important microbial sources for PHA production, use of conventional and non‐conventional substrates, product optimization using experimental design, metabolic engineering strategies, and global players in the commercialization of PHA in the past two decades. The challenges about PHA recovery and analysis have also been discussed which possess indirect hurdle while expanding the horizon of PHA‐based bioplastics. Summary Selection of appropriate microorganism and substrate plays a vital role in improving the productivity and characteristics of PHAs. Experimental design‐based bioprocess, use of metabolic engineering tools, and optimal product recovery techniques are invaluable in this dimension. Conclusion Optimization strategies, which are being explored in isolation, need to be logically integrated for the successful commercialization of microbial PHAs.

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Novel spectrophotometric technique for rapid determination of extractable PHA using Sudan black dye

Cited by 15 publications

References 18 publications

Vinasse: From A Residue To A High Added Value Biopolymer

Vinasse: From A Residue To A High Added Value Biopolymer

Haloarchaea as Cell Factories to Produce Bioplastics

Process optimization, metabolic engineering interventions and commercialization of microbial polyhydroxyalkanoates production – A state‐of‐the art review

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