Novel Smooth Muscle Cell Lines from Transgenic Mice Harboring Temperature-sensitive SV40 Large T-antigen Gene. Temperature-dependent Expression of Smooth Muscle Myosin Heavy Chain-1 and Calponin Genes

The immortalized cell lines with differentiation potentials: Their establishment and possible application

“…A novel smooth muscle cell line (SVS) established from the transgenic mice retains the expression of specific markers for smooth muscle cells, such as smooth muscle myosin-1, calponin and SM22α. (35) We found that one BM stromal cell line, TBR-B, can be induced to smooth muscle cells in the defined culture medium. (31) Since the differentiation stage of SVS and TBR-B cells can be controlled by culture temperature, they should be quite valuable tools to study the regulation of phenotypic modulation of smooth muscle cells, and ascorbic acid was shown to be a potent factor inducing the expression of h1-calponin and α-actin.…”

“…Type II, type XI, and type X-collagens, aggrecan, link protein Vascular smooth muscle cell lines (SVS30 and SVS24 cells) (35) α-actin, smooth muscle myosin heavy chain-1, and calponin…”

Benidipine, a Calcium Channel Blocker, Regulates Proliferation and Phenotype of Vascular Smooth Muscle Cells

“…Nifedipine (Wako, Osaka), rapamycin (Carbiochem, San Diego, CA, USA), ascorbic acid 2-phosphate (Sigma Aldrich, St. Louis, MO, USA), anti-α-actin monoclonal antibody (Sigma Aldrich), anti-calponin monoclonal antibody (Sigma Aldrich), anti-p21 monoclonal antibody (BD Biosciences, San Jose, CA, USA), anti-p70 S6 kinase antibody (C-18) (Santa Cruz, Santa Cruz, CA, USA), anti-phospho-p70 S6 Kinase (Thr389) antibody (Cell Signaling, Beverly, MA, USA), anti-S6 ribosomal protein antibody (Cell Signaling), anti-phospho-S6 ribosomal protein (Ser240 / 244) antibody (Cell Signaling), anti-mouse Ig, horseradish peroxidase linked whole antibody (Amersham Biosciences, Buckinghamshire, UK), p70 S6 kinase (T412E), active (Upstate, Lake Placid, NY, USA), S6 kinase assay kit (Upstate), and WST-1 (Roche, Basel, Switzerland). Anti-smooth muscle-specific myosin antibody was prepared as described by Hasegawa et al (16).…”

Conditionally immortalized brain capillary endothelial cell lines established from a transgenic mouse harboring temperature-sensitive simian virus 40 large T-antigen gene

“…However, at temperatures over 37°C large T-antigen becomes unstable and is degraded when cells are cultured (2); thus, the cells stop growing. Several cell lines, such as hepatocyte cells (3), gastric surface mucosal cells (4), and smooth muscle cells (5) have been established from Tg mice and these cell lines exhibit specific differentiated phenotypes. Moreover, this strategy is very useful for establishing cell lines from small organs and tissues, such as brain capillary endothelial cells.…”