Novel small molecules targeting bZIP23 TF improve stomatal conductance and photosynthesis under mild drought stress by regulating ABA

Vanitha, P. A.; Vijayaraghavareddy, Preethi; Uttarkar, Akshay; Dawane, Akashata; Sujitha, D.; Ashwin, V; Babitha, K. C.; Niranjan, Vidya; Sheshshayee, M. S.; Anuradha, Carani Venkatraman; Udayakumar, M.; Vemanna, Ramu S.

doi:10.1111/febs.16461

Cited by 19 publications

(6 citation statements)

References 44 publications

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“…The lack of an eff ect of treatments on the F v /F m ratio indicates that both eCO 2 and water defi cit did not alter leaf photochemistry, which was also observed in other species subjected to water defi cit, and indicates absence of pre-dawn photoinhibition (Rascher et al, 2004;Kitao et al, 2007;Oliveira and Marenco, 2019). A decline in Φ PSII under water defi cit (and hence an increase in NPQ) can be associated with a reduction in stomatal conductance and photosynthesis, as there is a direct relationship between stomatal conductance and Φ PSII (Jiang et al, 2006;Ahanger et al, 2020;Vanitha et al, 2022). In addition, water defi cit can eventually aff ect the stability of membranes in chloroplasts.…”

Section: Discussionmentioning

confidence: 53%

Elevated CO2 induces down-regulation of photosynthesis and alleviates the effect of water deficit in Ceiba pentandra (Malvaceae)

Silveira,

Marenco

2023

Rev. Árvore

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The simultaneous effect of elevated CO2 concentration and drought on trees is still under investigation in the Amazon. We evaluated the effect of CO2 levels (400 and 800 ppm) and water regimes (50% and 100% soil field capacity) on photosynthetic traits, chlorophyll fluorescence, and total biomass accumulation in Ceiba pentandra. In well-watered plants, light-saturated photosynthesis (PN-sat) increased in plants exposed to elevated CO2, but both PN-sat and stomatal conductance decreased in response to water deficit. The maximum carboxylation rate of Rubisco declined under elevated CO2, which indicates down-regulation of photosynthesis at elevated CO2. The Fv/Fm ratio was not affected by treatments. Notwithstanding, total plant biomass and leaf area were reduced by 34-37% under water deficit, but they were not affected by CO2 levels. The PN-sat values measured in well-irrigated plants at ambient CO2 were similar to those observed in plants subjected to elevated CO2 and water deficit (p = 0.26). We concluded that the effect of water deficit on PN-sat was mitigated by elevated CO2. These results suggest that the increase of atmospheric CO2 concentrations associated to climate changes can at least partly offset the negative effect of drought in this multiuse and widely distributed species.

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Section: Discussionmentioning

confidence: 53%

Elevated CO2 induces down-regulation of photosynthesis and alleviates the effect of water deficit in Ceiba pentandra (Malvaceae)

Silveira,

Marenco

2023

Rev. Árvore

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“…We hypothesize that stress‐induced transcripts of negative regulators may be efficiently targeted through dsRNA. OsbZIP23 transcription factor is upregulated in salt, drought and oxidative stress conditions (Pa et al., 2022; Sujitha et al., 2023). OsbZIP23 was therefore targeted by dsRNA.…”

Section: Resultsmentioning

confidence: 99%

Exogenous application of nanocarrier‐mediated double‐stranded RNA manipulates physiological traits and defence response against bacterial diseases

Pal,

Ingole,

Yavvari

et al. 2024

Molecular Plant Pathology

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Stability and delivery are major challenges associated with exogenous double‐stranded RNA (dsRNA) application into plants. We report the encapsulation and delivery of dsRNA in cationic poly‐aspartic acid‐derived polymer (CPP6) into plant cells. CPP6 stabilizes the dsRNAs during long exposure at varied temperatures and pH, and protects against RNase A degradation. CPP6 helps dsRNA uptake through roots or foliar spray and facilitates systemic movement to induce endogenous gene silencing. The fluorescence of Arabidopsis GFP‐overexpressing transgenic plants was significantly reduced after infiltration with gfp‐dsRNA‐CPP6 by silencing of the transgene compared to plants treated only with gfp‐dsRNA. The plant endogenous genes flowering locus T (FT) and phytochrome interacting factor 4 (PIF4) were downregulated by a foliar spray of ft‐dsRNA‐CPP6 and pif4‐dsRNA‐CPP6 in Arabidopsis, with delayed flowering and enhanced biomass. The rice PDS gene targeted by pds‐dsRNA‐CPP6 through root uptake was effectively silenced and plants showed a dwarf and albino phenotype. The NaCl‐induced OsbZIP23 was targeted through root uptake of bzip23‐dsRNA‐CPP6 and showed reduced transcripts and seedling growth compared to treatment with naked dsRNA. The negative regulators of plant defence SDIR1 and SWEET14 were targeted through foliar spray to provide durable resistance against bacterial leaf blight disease caused by Xanthomonas oryzae pv. oryzae (Xoo). Overall, the study demonstrates that transient silencing of plant endogenous genes using polymer‐encapsulated dsRNA provides prolonged and durable resistance against Xoo, which could be a promising tool for crop protection and for sustaining productivity.

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“…In the final stage, extra-precision docking was carried out using the same parameters as above and the best scoring states were taken as the final docking results [ 60 , 61 , 62 ].…”

Section: Methodsmentioning

confidence: 99%

Mycobacterium Time-Series Genome Analysis Identifies AAC2′ as a Potential Drug Target with Naloxone Showing Potential Bait Drug Synergism

Niranjan¹,

Uttarkar²,

Murali³

et al. 2022

Molecules

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The World Health Organization has put drug resistance in tuberculosis on its list of significant threats, with a critical emphasis on resolving the genetic differences in Mycobacterium tuberculosis. This provides an opportunity for a better understanding of the evolutionary progression leading to anti-microbial resistance. Anti-microbial resistance has a great impact on the economic stability of the global healthcare sector. We performed a timeline genomic analysis from 2003 to 2021 of 578 mycobacterium genomes to understand the pattern underlying genomic variations. Potential drug targets based on functional annotation was subjected to pharmacophore-based screening of FDA-approved phyto-actives. Reaction search, MD simulations, and metadynamics studies were performed. A total of 4,76,063 mutations with a transition/transversion ratio of 0.448 was observed. The top 10 proteins with the least number of mutations were high-confidence drug targets. Aminoglycoside 2’-N-acetyltransferase protein (AAC2’), conferring resistance to aminoglycosides, was shortlisted as a potential drug target based on its function and role in bait drug synergism. Gentamicin-AAC2’ binding pose was used as a pharmacophore template to screen 10,570 phyto-actives. A total of 66 potential hits were docked to obtain naloxone as a lead — active with a docking score of -6.317. Naloxone is an FDA-approved drug that rapidly reverses opioid overdose. This is a classic case of a repurposed phyto-active. Naloxone consists of an amine group, but the addition of the acetyl group is unfavorable, with a reaction energy of 612.248 kcal/mol. With gentamicin as a positive control, molecular dynamic simulation studies were performed for 200 ns to check the stability of binding. Metadynamics-based studies were carried out to compare unbinding energy with gentamicin. The unbinding energies were found to be −68 and −74 kcal/mol for naloxone and gentamycin, respectively. This study identifies naloxone as a potential drug candidate for a bait drug synergistic approach against Mycobacterium tuberculosis.

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Novel small molecules targeting bZIP23 TF improve stomatal conductance and photosynthesis under mild drought stress by regulating ABA

Cited by 19 publications

References 44 publications

Elevated CO2 induces down-regulation of photosynthesis and alleviates the effect of water deficit in Ceiba pentandra (Malvaceae)

Elevated CO2 induces down-regulation of photosynthesis and alleviates the effect of water deficit in Ceiba pentandra (Malvaceae)

Exogenous application of nanocarrier‐mediated double‐stranded RNA manipulates physiological traits and defence response against bacterial diseases

Mycobacterium Time-Series Genome Analysis Identifies AAC2′ as a Potential Drug Target with Naloxone Showing Potential Bait Drug Synergism

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