Novel, silver-ion-releasing nanofibrous scaffolds exhibit excellent antibacterial efficacy without the use of silver nanoparticles

Mohiti-Asli, Mahsa; Pourdeyhimi, Behnam; Loboa, Elizabeth G.

doi:10.1016/j.actbio.2013.12.024

Cited by 61 publications

(58 citation statements)

References 30 publications

(28 reference statements)

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“…SL at 0.5 mg/ml was slightly cytotoxic and was selected to further investigate the SL antimicrobial activity on Aa bacteria. Our results are in line with other findings in which biomaterials containing proper concentrations of Ag were shown to be compatible with eukaryotic cells, such as fibroblasts and osteoblasts …”

Section: Discussionsupporting

confidence: 93%

Gingival Mesenchymal Stem Cell (GMSC) Delivery System Based on RGD‐Coupled Alginate Hydrogel with Antimicrobial Properties: A Novel Treatment Modality for Peri‐Implantitis

Diniz

Chen

Ansari

et al. 2015

Journal of Prosthodontics

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Purpose Peri-implantitis is one of the most common inflammatory complications in dental implantology. Similar to periodontitis, in peri-implantitis, destructive inflammatory changes take place in the tissues surrounding a dental implant. Bacterial flora at the failing implant sites resemble the pathogens in periodontal disease and consist of Gram-negative anaerobic bacteria including Aggregatibacter actinomycetemcomitans (Aa). Here we demonstrate the effectiveness of a silver lactate (SL)-containing RGD-coupled alginate hydrogel scaffold as a promising stem cell delivery vehicle with antimicrobial properties. Materials and Methods Gingival mesenchymal stem cells (GMSCs) or human bone marrow mesenchymal stem cells (hBMMSCs) were encapsulated in SL-loaded alginate hydrogel microspheres. Stem cell viability, proliferation, and osteo-differentiation capacity were analyzed. Results Our results showed that SL exhibited antimicrobial properties against Aa in a dose-dependent manner, with 0.50 mg/ml showing the greatest antimicrobial properties while still maintaining cell viability. At this concentration, SL-containing alginate hydrogel was able to inhibit Aa on the surface of Ti discs and significantly reduce the bacterial load in Aa suspensions. Silver ions were effectively released from the SL-loaded alginate microspheres for up to 2 weeks. Osteogenic differentiation of GMSCs and hBMMSCs encapsulated in the SL-loaded alginate microspheres were confirmed by the intense mineral matrix deposition and high expression of osteogenesis-related genes. Conclusion Taken together, our findings confirm that GMSCs encapsulated in RGD-modified alginate hydrogel containing SL show promise for bone tissue engineering with antimicrobial properties against Aa bacteria in vitro.

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Section: Discussionsupporting

confidence: 93%

Gingival Mesenchymal Stem Cell (GMSC) Delivery System Based on RGD‐Coupled Alginate Hydrogel with Antimicrobial Properties: A Novel Treatment Modality for Peri‐Implantitis

Diniz

Chen

Ansari

et al. 2015

Journal of Prosthodontics

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“…The electrospun nanofiber can mimic the extra cellular matrix (ECM) environment which will help the host cells to grow and create a new natural cellular matrix [21,22]. Most importantly the preferred therapeutic materials can be introduced to the nanofiber matrix using the electrospinning process [17,23,24], that can seriously affect the wound healing.…”

Section: Q2mentioning

confidence: 99%

Electrospun polyurethane-dextran nanofiber mats loaded with Estradiol for post-menopausal wound dressing

Unnithan

Sasikala

Murugesan

et al. 2015

International Journal of Biological Macromolecules

123

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“…Compared to traditional wound dressings, fiber-based wound dressings have a number of advantages such as haemostasis, high filtration, semi-permeability, conformability, functional ability and improved cosmetic appearance/scar free (23). However, the current available studies on electrospun fibers for wound dressing are limited to the use of surface modifications or incorporation with chitosan, Ag ions/nanoparticles, ZnO nanoparticles, and antibiotics (24-27). In this work, we hypothesized that electrospun fiber membranes could provide sustained release of 25-hydroxyvitamin D 3 .…”

Section: Introductionmentioning

confidence: 99%

Local Sustained Delivery of 25-Hydroxyvitamin D3 for Production of Antimicrobial Peptides

et al. 2015

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Purpose-This study seeks to develop fiber membranes for local sustained delivery of 25-hydroxyvitamin D 3 to induce the expression and secretion of LL-37 at or near the surgical site, which provides a novel therapeutic approach to minimize the risk of infections.Methods-25-hydroxyvitamin D 3 loaded poly( L -lactide) (PLA) and poly(ε-caprolactone) (PCL) fibers were produced by electrospinning. The morphology of obtained fibers was characterized using atomic force microscope (AFM) and scanning electron microscope (SEM). 25-hydroxyvitamin D 3 releasing kinetics were quantified by enzyme-linked immunosorbent assay (ELISA) kit. The expression of cathelicidin (hCAP 18) and LL-37 was analyzed by immunofluorescence staining and ELISA kit. The antibacterial activity test was conducted by incubating pseudomonas aeruginosa in a monocytes' lysis solution.Results-AFM images suggest that the surface of PCL fibers is smooth, however, the surface of PLA fibers is relatively rough, in particular, after encapsulation of 25-hydroxyvitamin D 3 . The duration of 25-hydroxyvitamin D 3 release can last more than 4 weeks for all the tested samples. Plasma treatment can promote the release rate of 25-hydroxyvitamin D 3 . Human keratinocytes and monocytes express significantly higher levels of hCAP18/LL-37 after incubation with plasma treated and 25-hydroxyvitamin D 3 loaded PCL fibers than the cells incubated with around 10 times amount of free drug. After incubation with this fiber formulation for 5 days LL-37 in the lysis solutions of U937 cells can effectively kill the bacteria.

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Novel, silver-ion-releasing nanofibrous scaffolds exhibit excellent antibacterial efficacy without the use of silver nanoparticles

Cited by 61 publications

References 30 publications

Gingival Mesenchymal Stem Cell (GMSC) Delivery System Based on RGD‐Coupled Alginate Hydrogel with Antimicrobial Properties: A Novel Treatment Modality for Peri‐Implantitis

Gingival Mesenchymal Stem Cell (GMSC) Delivery System Based on RGD‐Coupled Alginate Hydrogel with Antimicrobial Properties: A Novel Treatment Modality for Peri‐Implantitis

Electrospun polyurethane-dextran nanofiber mats loaded with Estradiol for post-menopausal wound dressing

Local Sustained Delivery of 25-Hydroxyvitamin D3 for Production of Antimicrobial Peptides

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