Novel serological tools for detection of Thottapalayam virus, a Soricomorpha-borne hantavirus

Schlegel, Mathias; Tegshduuren, Erdenesaikhan; Yoshimatsu, Kumiko; Petraityte, Rasa; Sasnauskas, Kęstutis; Hammerschmidt, Bärbel; Friedrich, R; Mertens, Marc; Groschup, Martin H.; Arai, Satoru; Endo, Rika; Shimizu, Kenta; Koma, Takaaki; Yasuda, Shumpei P.; Ishihara, Chiaki; Ulrich, Rainer G.; Arikawa, Jiro; Köllner, Bernd

doi:10.1007/s00705-012-1405-9

Cited by 16 publications

(25 citation statements)

References 49 publications

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“…The reactivity of the same antibodies was also studied by IFA. PUUV N protein was recognized by mAbs 4C3, 2E12 and E5G6 as previously reported (Lundkvist et al, 1991;Schlegel et al, 2012a;Yoshimatsu et al, 1996), but none of the mAbs reacted with SWSV N protein. However, the polyclonal rabbit antiserum against the first 120 aa of SWSV N protein reacted with PUUV N protein in immunoblotting, further demonstrating the antigenic similarity of this region (Fig.…”

Section: Resultssupporting

confidence: 83%

“…Once we had established and characterized the recombinant SWSV N protein ELISA for its cross-reactivity, we decided to use this test to study whether SWSV could be The mAbs for PUUV, 4C3 and 2E12 are described in Lundkvist et al (1991); TULV, 3C11 and 3F10 are described in Vapalahti et al (1995b); TMPV, 2D9, 1F1, 2H6 and 2E7 are described in Schlegel et al (2012a); HTNV, ECO2, E5G6 and F23A1 are described in Yoshimatsu et al (1996). The polyclonal antibodies for PUUV and SWSV have been described previously (Ling et al, 2014;Vapalahti et al, 1995b).…”

Section: Resultsmentioning

confidence: 99%

“…This essential structural motif is also found in the N protein of the prototype shrew-borne hantavirus, Thottapalayam virus (TMPV, carried by Suncus murinus). TMPV, found in Asia, is distant from rodent-borne hantaviruses in phylogeny and the N protein of TMPV has been reported not to react with antiserum against rodent-borne hantaviruses (Schlegel et al, 2012a;Song et al, 2007a).…”

Section: Introductionmentioning

confidence: 99%

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Serological survey of Seewis virus antibodies in patients suspected for hantavirus infection in Finland; a cross-reaction between Puumala virus antiserum with Seewis virus N protein?

Ling

Vaheri

Hepojoki

et al. 2015

Journal of General Virology

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Puumala virus (PUUV, carried by Myodes glareolus) co-circulates with Seewis virus (SWSV, carried by Sorex araneus) in Finland. While PUUV causes 1000-3000 nephropathia epidemica (NE) cases annually, the pathogenicity of SWSV to man is unknown. To study the prevalence of SWSV antibodies in hantavirus fever-like patients' sera, we used recombinant SWSV nucleocapsid (N) protein as the antigen in ELISA, immunofluorescence assay (IFA) and immunoblotting. While characterizing the recombinant SWSV N protein, we observed that a polyclonal rabbit antiserum against PUUV N protein cross-reacted with SWSV N protein and vice versa. We initially screened 486 (450 PUUV-seronegative and 36 PUUV-seropositive) samples sent to Helsinki University Hospital Laboratory for PUUV serodiagnosis during 2002 and 2007 in an SWSV N protein IgG ELISA. In total, 4.2 % (19/450) of the PUUVseronegative samples were reactive in the SWSV N protein IgG ELISA and none of the tested samples [43 PUUV-seronegative (weakly reactive in the SWSV IgG ELISA) and 15 random] were reactive in the SWSV N protein IgM ELISA. None of the IgG reactions could be confirmed by IFA or immunoblotting. Furthermore, among the 36 PUUV-seropositive samples three were reactive in SWSV N protein IgG and ten in SWSV N protein IgM ELISA. One PUUVseropositive sample reacted with SWSV N protein in IFA and four in immunoblotting. Finally, we applied competitive ELISA to confirm that the observed reactivity was due to cross-reactivity rather than a true SWSV response. In conclusion, no evidence of SWSV infection was found among the 486 samples studied; however, we did demonstrate that PUUV antiserum cross-reacted with shrew-borne hantavirus N protein.

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Section: Resultssupporting

confidence: 83%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Serological survey of Seewis virus antibodies in patients suspected for hantavirus infection in Finland; a cross-reaction between Puumala virus antiserum with Seewis virus N protein?

Ling

Vaheri

Hepojoki

et al. 2015

Journal of General Virology

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“…Shigel et al reported MAbs against TPMV [34]. All four clones were directed against the N-terminal region.…”

Section: Hantavirus N Proteinmentioning

confidence: 99%

“…Furthermore, the N-terminal region showed strong reactivity in a Western blot assay on staining by MAbs and polyclonal antisera. This indicated that the epitope found in the N terminus is linear and immundominant [34]. Similarly, antibody epitopes induced in HFRS and HPS patient sera and rodent-borne hantavirus-infected animals using a synthetic peptide antigen and/or truncated antigens were investigated.…”

Section: Hantavirus N Proteinmentioning

confidence: 99%

Antigenic Properties of N Protein of Hantavirus

Yoshimatsu

Arikawa

2014

Viruses

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Hantavirus causes two important rodent-borne viral zoonoses, hemorrhagic fever with renal syndrome (HFRS) in Eurasia and hantavirus pulmonary syndrome (HPS) in North and South America. Twenty-four species that represent sero- and genotypes have been registered within the genus Hantavirus by the International Committee on Taxonomy of Viruses (ICTV). Among the viral proteins, nucleocapsid (N) protein possesses an immunodominant antigen. The antigenicitiy of N protein is conserved compared with that of envelope glycoproteins. Therefore, N protein has been used for serological diagnoses and seroepidemiological studies. An understanding of the antigenic properties of N protein is important for the interpretation of results from serological tests using N antigen. N protein consists of about 430 amino acids and possesses various epitopes. The N-terminal quarter of N protein bears linear and immunodominant epitopes. However, a serotype-specific and multimerization-dependent antigenic site was found in the C-terminal half of N protein. In this paper, the structure, function, and antigenicity of N protein are reviewed.

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Epidemiological Surveillance of Viral Hemorrhagic Fevers With Emphasis on Clinical Virology

Montoya-Ruiz

Rodas

2017

Methods in Molecular Biology

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This article will outline surveillance approaches for viral hemorrhagic fevers. Specific methods for surveillance of clinical samples will be emphasized. Separate articles will describe methods for surveillance of rodent-borne viruses (roboviruses) and arthropod-borne viruses (arboviruses). Since the appearance of hantaviruses and arenaviruses in the Americas, more than 30 different species in each group have been established, and therefore they have become the most frequently emerging viruses. Flaviviruses such as yellow fever and dengue viruses, although easier to recognize, are also more widely spread and therefore considered a very important public health issue, particularly for under-developed countries. On the other hand, marburgviruses and ebolaviruses, previously thought to be restricted to the African continent, have recently been shown to be more global. For many of these agents virus isolation has been a challenging task: trapping the specific vectors (mosquitoes and ticks), and reservoirs (rodents and bats), or obtaining the samples from suspected clinical human cases demands special protective gear, uncommon devices (respirators), special facilities (BSL-3 and 4), and particular skills to recognize the slow and inapparent cytopathic effects in cell culture. Alternatively, serological and molecular approaches have been very helpful in discovering and describing newly emerging viruses in many areas where the previous resources are unavailable. Unfortunately, in many cases, detailed studies have been performed only after outbreaks occur, and then active surveillance is needed to prevent viral dissemination in human populations.

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Novel serological tools for detection of Thottapalayam virus, a Soricomorpha-borne hantavirus

Cited by 16 publications

References 49 publications

Serological survey of Seewis virus antibodies in patients suspected for hantavirus infection in Finland; a cross-reaction between Puumala virus antiserum with Seewis virus N protein?

Serological survey of Seewis virus antibodies in patients suspected for hantavirus infection in Finland; a cross-reaction between Puumala virus antiserum with Seewis virus N protein?

Antigenic Properties of N Protein of Hantavirus

Epidemiological Surveillance of Viral Hemorrhagic Fevers With Emphasis on Clinical Virology

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