Novel Sensitive, Two-site ELISA for the Quantification of Der f 1 Using Monoclonal Antibodies

Kim, Ji Tae; Lee, Jae Hyun; Yuk, Jie Eun; Song, Hangyeol; Kim, Hyunho; Kim, Sung Hyun; Kim, Dong Jun; Shin, Yeji; Lee, Deug Chan; Jeong, Kyoung Yong

doi:10.4168/aair.2021.13.4.665

Cited by 2 publications

(2 citation statements)

References 7 publications

(7 reference statements)

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“…ELISA has been generated from a vast search for substitutes to labeling of antigens or antibodies with radioisotopes for application in immunoradiometric techniques (Exner & Koutts, 2021) and radioimmunoassays (Tamura et al., 2021). The two‐site ELISA, also known as sandwich ELISA, has been used for bacteria's recognition in foodstuff (Kim et al., 2021). This technique has included the capture of antigen via an antibody that has been immobilized on a solid matrix.…”

Section: Common Methods For Detection Of Foodborne Pathogenic Agentsmentioning

confidence: 99%

State of the art: Lateral flow assays toward the point‐of‐care foodborne pathogenic bacteria detection in food samples

Sohrabi

Majidi

Khaki

et al. 2022

Comp Rev Food Sci Food Safe

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Diverse chemicals and some physical phenomena recently introduced in nanotechnology have enabled scientists to develop useful devices in the field of food sciences. Concerning such developments, detecting foodborne pathogenic bacteria is now an important issue. These kinds of bacteria species have demonstrated severe health effects after consuming foods and high mortality related to acute cases. The most leading path of intoxication and infection has been through food matrices. Hence, quick recognition of foodborne bacteria agents at low concentrations has been required in current diagnostics. Lateral flow assays (LFAs) are one of the urgent and prevalently applied quick recognition methods that have been settled for recognizing diverse types of analytes. Thus, the present review has stressed on latest developments in LFAs‐based platforms to detect various foodborne pathogenic bacteria such as Salmonella, Listeria, Escherichia coli, Brucella, Shigella, Staphylococcus aureus, Clostridium botulinum, and Vibrio cholera. Proper prominence has been given on exactly how the labels, detection elements, or procedures have affected recent developments in the evaluation of diverse bacteria using LFAs. Additionally, the modifications in assays specificity and sensitivity consistent with applied food processing techniques have been discussed. Finally, a conclusion has been drawn for highlighting the main challenges confronted through this method and offered a view and insight of thoughts for its further development in the future.

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Section: Common Methods For Detection Of Foodborne Pathogenic Agentsmentioning

confidence: 99%

State of the art: Lateral flow assays toward the point‐of‐care foodborne pathogenic bacteria detection in food samples

Sohrabi

Majidi

Khaki

et al. 2022

Comp Rev Food Sci Food Safe

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“…However, the purification of natural allergens from their original allergen sources can be rather difficult, requiring extensive purification steps to achieve real purity [45][46][47]. Additionally, the number of monoclonal antibodies available for use in affinity chromatography is currently scarce, especially in the case of certain allergic sources [48,49]. For this reason, most research groups tend to heterologously express allergens and characterize them in vitro first [16,19,26].…”

Section: Discussionmentioning

confidence: 99%

Removal of N-Terminal Peptide Impacts Structural Aspects of an IgE-Reactive Recombinant Der p 5

Vieira,

Silva,

Silveira

et al. 2023

Allergies

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(1) Background: Modification of the structural elements of allergens is widely used in the field of allergies. The goal of the present research was to express, purify, and characterize the shortened recombinant group 5 allergen of Dermatophagoides pteronyssinus (rDer p 5). (2) Methods: rDer p 5 storage stability and aggregation capacity were explored through in silico analysis, dynamic light scattering (DLS), and SDS-PAGE. Serum IgE reactivity and cytokine amount were investigated in sera or cell culture supernatants through ELISA, MULTIPLEX®, and Western blot analysis using sera from sensitized humans from Brazil, Colombia, and Ecuador. (3) Results: Dimeric rDer p 5 was detected through native PAGE, and this result was confirmed by data from DLS. The protein was thermically stable, as it did not degrade at 4 °C for 21 days. The shortened rDer p 5 was classified as a major IgE allergen in Brazil and Colombia, but minor in Ecuador. IL-13, IL-10, IL-1β, and IL-6 were significantly elevated in the sera of rDer p 5-reactive patients. The same cytokines plus IL-5 were more secreted by human cells upon rDer p 5 stimulation. (4) Conclusions: N-terminal peptide deletion led to a higher rDer p 5 folding stability, which, even though dimeric, was an IgE-reactive protein. Therefore, rDer p 5 could be used for molecular diagnostic applications or as backbone for hypoallergen design.

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Novel Sensitive, Two-site ELISA for the Quantification of Der f 1 Using Monoclonal Antibodies

Cited by 2 publications

References 7 publications

State of the art: Lateral flow assays toward the point‐of‐care foodborne pathogenic bacteria detection in food samples

State of the art: Lateral flow assays toward the point‐of‐care foodborne pathogenic bacteria detection in food samples

Removal of N-Terminal Peptide Impacts Structural Aspects of an IgE-Reactive Recombinant Der p 5

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