Novel sensitive immunosensor for the selective detection of Engrailed 2 urinary prostate cancer biomarker

Mokni, Meriem; Tlili, Amal; Attia, Ghada; Khaoulani, Sohayb; Zerrouki, Chouki; Omezzine, Asma; Othmane, Ali; Bouslama, Ali; Fourati, Najla

doi:10.1016/j.bios.2022.114678

Cited by 8 publications

(4 citation statements)

References 26 publications

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“…Fitting the measurement data according to Hill Model, the dissociation constant is 𝐾 𝑑 = 4.605 × 10 −10 mg/mL. The value range of the reported 𝐾 𝑑 is very low, indicating a solid attachment in this recognition system, which is expected for antibody-antigen immunoreaction [74].…”

Section: Immunosensor Analytical Performancementioning

confidence: 93%

Ultra-sensitive selective detection of HopQ protein as a biomarker for Helicobacter pylori bacteria by an electrochemical voltammetric sensor

Jaradat¹,

Al‐Hamry

Ibbini

et al. 2023

Preprint

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Background Helicobacter pylori (H. pylori) is a highly contagious pathogenic bacterium that can cause gastrointestinal ulcers and may gradually lead to gastric cancer. H. pylori expresses the outer membrane HopQ protein at the earliest stages of infection. Therefore, HopQ is a highly reliable candidate as a biomarker for H. pylori detection in saliva samples. Materials and Methods: An H. pylori immunosensor is developed based on detecting HopQ as a biomarker in saliva by a screen-printed carbon electrode (SPCE) modified with MWCNT-COOH decorated with gold nanoparticles (AuNP). The HopQ antibodies are grafted on the SPCE/MWCNT/AuNP surface using EDC/S-NHS chemistry. The sensor performance is investigated by various methods and H. pylori detection performance in spiked saliva samples is evaluated by square wave voltammetry. Results: The sensor is suitable for HopQ detection with high sensitivity and excellent linearity in the 10 pg/mL - 100 ng/mL range and with a 10 pg/ml limit of detection. The sensor was tested in saliva at 10 ng/mL and returned an 107.6% recovery. The dissociation constant Kd for HopQ/HopQ antibody interaction, estimated from Hill's model, is calculated with a value of an order of 4.605 × 10−10 mg/mL. Conclusions: Due to the strategical choice of biomarker, the utilization of nanocomposite material to enhance the SPCE electrical performance, the intrinsic selectivity of the antibody-antigen interaction, and effective immobilization, the fabricated platform shows high selectivity, good stability, reproducibility, and cost-effectiveness for early H. pylori detection. Additionally, we provide insight into possible future aspects the researchers are recommended to focus on.

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Section: Immunosensor Analytical Performancementioning

confidence: 93%

Ultra-sensitive selective detection of HopQ protein as a biomarker for Helicobacter pylori bacteria by an electrochemical voltammetric sensor

Jaradat¹,

Al‐Hamry

Ibbini

et al. 2023

Preprint

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“…The gold electrodes underwent initial cleaning using a polishing process outlined in our previous publication [54]. Two different thiolated single-stranded DNA probes with 40 bases, namely, Probe 1 and Probe 2, were designed to investigate the influence of hy bridization location on the detection signal and ensure the specific and selective recogni tion of PCA3.…”

Section: Genosensor Designmentioning

confidence: 99%

“…To understand the affinity between PCA3 and Probe 2, the dissociation constant (K d ) was estimated using a combined power law and Hill model, as outlined in Equation (3). The power law component models non-specific adsorption, while the Hill equation component accounts for specific recognition processes [54,70]. This approach provides a comprehensive analysis of both non-specific and specific interactions between PCA3 and the probe:…”

Section: Specificity Testsmentioning

confidence: 99%

Designing a Simple Electrochemical Genosensor for the Detection of Urinary PCA3, a Prostate Cancer Biomarker

Mokni,

Tlili,

Khalij

et al. 2024

Micromachines

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This study investigates the feasibility of a simple electrochemical detection of Prostate Cancer Antigen 3 (PCA3) fragments extracted from patients’ urine, using a thiolated single-strand DNA probe immobilized on a gold surface without using a redox probe. To enhance the PCA3 recognition process, we conducted a comparative analysis of the hybridization location using two thiolated DNA probes: Probe 1 targets the first 40 bases, while Probe 2 targets the fragment from bases 47 to 86. Hybridization with PCA3 followed, using square wave voltammetry. The limit of detection of the designed genosenors were of the order of (2.2 ng/mL), and (1.6 ng/mL) for Probes 1 and 2, respectively, and the subsequent sensitivities were of the order of (0.09 ± 0.01) µA−1 · µg−1 · mL and (0.10 ± 0.01) µA−1 · µg−1 · mL. Specificity tests were then conducted with the sensor functionalized with Probe 2, as it presents better analytical performances. The electrochemical results indicate that the designed sensor can clearly discriminate a complementary target from a non-complementary one. A further modeling of the calibration curves with the Power Law/Hill model indicates that the dissociation constant increases by one order of magnitude, confirming the ability of the designed sensor to perfectly discriminate complementary targets from non-complementary ones.

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“…It has been reported that EN1 is overexpressed in tumors and functions as an oncogene 3–6 . High‐expression levels of EN2 have been found in many solid cancers, including prostate, bladder, colorectal, and breast cancer 7–11 . There is accumulating evidence that EN2 is a potential oncogene; however, the exact mechanism underlying its biological function remains to be fully elucidated.…”

Section: Introductionmentioning

confidence: 99%

Engrailed 2 serves as a master regulator of the super‐enhancer in the TNC gene locus in non‐small cell lung cancer

Li,

Jiang,

Wang

et al. 2023

Environmental Toxicology

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Engrailed 2 (EN2) is a homeodomain‐containing protein that is dysregulated in many types of cancer. However, the role of EN2 in non‐small cell lung cancer (NSCLC) and the mechanism underlying its biological function are largely unclear. Here, we showed that EN2 played an oncogenic function in NSCLC and greatly enhanced the malignant phenotype of NSCLC cells. Meanwhile, EN2 was able to boost the expression of a well‐studied oncogenic Tenascin‐C (TNC) gene, which in turn activated the AKT signaling pathway. Interestingly, we found that EN2 directly bound to the super‐enhancer (SE) region in the TNC locus. The histone marker H3K27ac was also enriched in the region, indicating the activation of the SE. Treatment of the cells with JQ1, an inhibitor of SE activity, abrogated the effect of EN2 on the expression of TNC and phosphorylation of AKT‐Ser473. Collectively, our work unveils a novel mode of EN2 function, in which EN2 governs the SE in the TNC locus, consequently activating the oncogenic TNC–AKT axis in NSCLC.

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Novel sensitive immunosensor for the selective detection of Engrailed 2 urinary prostate cancer biomarker

Cited by 8 publications

References 26 publications

Ultra-sensitive selective detection of HopQ protein as a biomarker for Helicobacter pylori bacteria by an electrochemical voltammetric sensor

Ultra-sensitive selective detection of HopQ protein as a biomarker for Helicobacter pylori bacteria by an electrochemical voltammetric sensor

Designing a Simple Electrochemical Genosensor for the Detection of Urinary PCA3, a Prostate Cancer Biomarker

Engrailed 2 serves as a master regulator of the super‐enhancer in the TNC gene locus in non‐small cell lung cancer

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