Novel scanning magnetic microscopy method for the characterization of magnetic nanoparticles

Araújo, Jefferson F.D.F.; Tahir,; Arsalani, Soudabeh; Freire, F.L.; Mariotto, G.; Cremona, Marco; Mendoza, Leonardo Alfredo Forero; Luz-Lima, C.; Zaman, Quaid; Rosso, Tommaso Del; Baffa, Oswaldo; Bruno, A. C.

doi:10.1016/j.jmmm.2019.166300

Cited by 20 publications

(14 citation statements)

References 29 publications

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“…The transmission electron microscopy (TEM) image of sample 3 (Fig. 1c) reveals nanoparticles of spherical-like shape, corroborating previous studies (Harush et al 2008;Araujo et al 2020;Rocha et al 2017), measuring an average size of 34 ± 9 nm, the latter being determined from the size distribution fitted by a log-normal function as shown in Fig. 1d.…”

Section: Characterization Of the Magcit-mb Nanosystemsupporting

confidence: 89%

Methylene blue associated with maghemite nanoparticles has antitumor activity in breast and ovarian carcinoma cell lines

et al. 2021

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Background Cancer constitutes group of diseases responsible for the second largest cause of global death, and it is currently considered one of the main public health concerns nowadays. Early diagnosis associated with the best choice of therapeutic strategy, is essential to achieve success in cancer treatment. In women, breast cancer is the second most common type, whereas ovarian cancer has the highest lethality when compared to other neoplasms of the female genital system. The present work, therefore, proposes the association of methylene blue with citrate-coated maghemite nanoparticles (MAGCIT–MB) as a nanocomplex for the treatment of breast and ovarian cancer. Results In vitro studies showed that T-47D and A2780 cancer cell lines underwent a significant reduction in cell viability after treatment with MAGCIT–MB, an event not observed in non-tumor (HNTMC and HUVEC) cells and MDA-MB-231, a triple-negative breast cancer cell line. Flow cytometry experiments suggest that the main mechanism of endocytosis involved in the interiorization of MAGCIT–MB is the clathrin pathway, whereas both late apoptosis and necrosis are the main types of cell death caused by the nanocomplex. Scanning electron microscopy and light microscopy reveal significant changes in the cell morphology. Quantification of reactive oxygen species confirmed the MAGCIT–MB cytotoxic mechanism and its importance for the treatment of tumor cells. The lower cytotoxicity of individual solution of maghemite nanoparticles with citrate (MAGCIT) and free methylene blue (MB) shows that their association in the nanocomplex is responsible for its enhanced therapeutic potential in the treatment of breast and ovarian cancer in vitro. Conclusions Treatment with MAGCIT–MB induces the death of cancer cells but not normal cells. These results highlight the importance of the maghemite core for drug delivery and for increasing methylene blue activity, aiming at the treatment of breast and ovarian cancer. Graphic Abstract

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Section: Characterization Of the Magcit-mb Nanosystemsupporting

confidence: 89%

Methylene blue associated with maghemite nanoparticles has antitumor activity in breast and ovarian carcinoma cell lines

et al. 2021

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“…The magnetic maps were generated using a home-built SMM, with a reading system containing Hall effect sensors connected to a reading system [ 33 , 34 ]. The SMM technique consists of an improved method to produce magnetic maps for each magnetic field applied to the sample.…”

Section: Nanoparticles Characterizationmentioning

confidence: 99%

“…In recent years, the scanning magnetic microscope (SMM) has played a fundamental role in the characterization of magnetic materials, updated in the characterization of samples of several materials, such as rocks, steels, and nanostructures [ 33 , 34 , 35 ]. In the present work, we report a magnetization method improvement, where the maps obtained by a home-built SMM are three-dimensional (3D) instead of the in-line technique obtained through maps in two-dimensional (2D) mode, as previously developed by Araujo et al [ 33 ]. Parameters extracted from the SMM data can be used to build a MxH hysteresis curve with the respective magnetization values for each applied field.…”

Section: Introductionmentioning

confidence: 99%

Magnetic Characterization by Scanning Microscopy of Functionalized Iron Oxide Nanoparticles

et al. 2021

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This study aimed to systematically understand the magnetic properties of magnetite (Fe3O4) nanoparticles functionalized with different Pluronic F-127 surfactant concentrations (Fe3O4@Pluronic F-127) obtained by using an improved magnetic characterization method based on three-dimensional magnetic maps generated by scanning magnetic microscopy. Additionally, these Fe3O4 and Fe3O4@Pluronic F-127 nanoparticles, as promising systems for biomedical applications, were prepared by a wet chemical reaction. The magnetization curve was obtained through these three-dimensional maps, confirming that both Fe3O4 and Fe3O4@Pluronic F-127 nanoparticles have a superparamagnetic behavior. The as-prepared samples, stored at approximately 20 °C, showed no change in the magnetization curve even months after their generation, resulting in no nanoparticles free from oxidation, as Raman measurements have confirmed. Furthermore, by applying this magnetic technique, it was possible to estimate that the nanoparticles’ magnetic core diameter was about 5 nm. Our results were confirmed by comparison with other techniques, namely as transmission electron microscopy imaging and diffraction together with Raman spectroscopy. Finally, these results, in addition to validating scanning magnetic microscopy, also highlight its potential for a detailed magnetic characterization of nanoparticles.

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“…In this article, monodisperse magnetic nanoparticles (MNPs) of magnetite synthesized by co-precipitation were characterized by a magnetic method especially appropriate for low mass samples in the order of tens of micrograms. This method is based on scanning magnetic microscopy (SMM) and has been an important research tool for obtaining the magnetic properties of different materials and for their applications in areas such as geology, biomedicine, science and technology [1][2][3]. In this study, it was used a susceptometer composed by a reading system based on a gradiometric configuration using two Hall effect sensors made of GaIn with a magnetic detection diameter of 300 µm.…”

Section: Rio De Janeiro Brazilmentioning

confidence: 99%

“…1.b). In addition to characterizing the behavior of MNPs (superparamagnetic behavior) at room temperature, the average diameter (around 12 nm) was estimated based on the magnetization curve [3]. The average diameter of MNPs is usually obtained by using techniques such as TEM (FIG.…”

Section: Rio De Janeiro Brazilmentioning

confidence: 99%

Synthesis and Characterization of Monodisperse Magnetic Nanoparticles by a Scanning Susceptometer

et al. 2020

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Novel scanning magnetic microscopy method for the characterization of magnetic nanoparticles

Cited by 20 publications

References 29 publications

Methylene blue associated with maghemite nanoparticles has antitumor activity in breast and ovarian carcinoma cell lines

Methylene blue associated with maghemite nanoparticles has antitumor activity in breast and ovarian carcinoma cell lines

Magnetic Characterization by Scanning Microscopy of Functionalized Iron Oxide Nanoparticles

Synthesis and Characterization of Monodisperse Magnetic Nanoparticles by a Scanning Susceptometer

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