Novel route of infection in experimental model of hydatid disease

Kakru, Dalip K; Sofi, Bashir Ahmad; Assadullah, S

doi:10.4103/0377-4929.42514

Cited by 2 publications

(3 citation statements)

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“…Mice have been infected with a dose of ±2000 virulence of scoleces where Kakru et al [14] proved that injecting 40 mice in the peritoneal membrane with the number of 2000 of scoleces from the hydatid cysts fluid (that has been surgically removed from an infected being) caused 80% of infection, forming cysts that go between 1 -2 ml in diameter in the span of 2 -3 months in the mice. He also noticed meteorism in the stomach after 6 -7 months then the death of all mice.…”

Section: Discussionmentioning

confidence: 99%

“…Numerous samples of the hydatid cyst fluid have been extracted from hydatid cysts of camels and goats severally. The scoleces have been counted microscopically and a dose has been prepared according to Aboul-Atta et al [13] where a 0.5 ml of fluid contained ± 2000 of the scoleces that has been treated before usage by adding 100 units of penicillin with 200 ml/kg of streptomycin according to Kakru et al [14].…”

Section: Preparation Of the Sources Of Infectionmentioning

confidence: 99%

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Experiment of Hydatid Cyst in Two Strains (Camels and Goats) in Saudi Arabia

Bakhraibah¹,

Alsulami²,

Toulah³

et al. 2019

OJAS

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Hydatid cyst disease is one of the most parasitic diseases transmitted from carnivores animals (such as dogs) to humans and causes a deterioration in the general health. Its transmission to herbivores animals led to a substantial economic loss in the meat productivity and reduced its quality. The increase of acquisition of dogs at homes nowadays led to an increase in the risk of infection with many parasitic diseases such as hydatid cyst disease. The present study was conducted to provide a modern view on the current status of hydatid cyst disease among slaughtered animals in Jeddah through periodic visiting to a slaughter house in North Jeddah. The mice prepared have been infected in the peritoneal membrane with 0.2 ml of hydatid cyst fluid containing ±2000 protoscoleces from fertile cysts obtained from an infected goat's liver which resulted in a 75% infection rate. Mice infected with hydatid fluid from fertile cysts obtained from an infected camel's lungs showed a result of 11.1% infection rate. The cysts appeared in the peritoneal and abdominal cavity.

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Section: Discussionmentioning

confidence: 99%

Section: Preparation Of the Sources Of Infectionmentioning

confidence: 99%

Experiment of Hydatid Cyst in Two Strains (Camels and Goats) in Saudi Arabia

Bakhraibah¹,

Alsulami²,

Toulah³

et al. 2019

OJAS

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show abstract

“…Secondary hydatidosis was developed in five BALB/c female mice which were injected subcutaneously with 1000 freshly isolated PSCs prepared in 1ml PBS ( pH 7.2) when mice were two weeks old (Kakru et al, 2008). After four months, mice were killed by cervical dislocation and developing HCs were dissected out from subcutaneous tissue and maintained in RPMI-1640 medium.…”

Section: Parasitesmentioning

confidence: 99%

In Vitro Activity of Novel Metronidazole Derivatives on Larval Stages of Echinococcus Granulosus

Saadeh¹,

Nasr²,

Mubarak³

et al. 2014

JJBS

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The effects of metronidazole (MTZ) and novel synthesized MTZ derivatives on in vitro cultured Echinococcus granulosus protoscoleces (PSCs), 30 day old segmentation stage and hydatid cysts (HC) developing secondarily in BALB/c mice were compared to those caused upon treatment with comparable doses of albandazole (ABZ) and mebendazole (MBZ) drugs. The highest protoscolicidal action resulted from the use of a non-schiff based MTZ derivative (MTZ-w: 4-[2-(2-methyl-5nitro-1H-imidazol-1-yl) ethyoxy] benzeyldehyde). Incubation of PSCs with MTZ-w concentrations of 25, 12.5 and 6.25 µg/ml resulted in significantly higher mortality rates than those caused by ABZ or MBZ at all periods post incubation. Total mortality of PSCs always occurred one day earlier using MTZ-w. Moreover, incubation of PSCs with MTZ-w at 6.25 µg/ml concentration resulted in greater mortality of PSCs than that caused by ABZ at 25ug/ml concentration. Three other MTZ derivatives showed similar in vitro effects on PSCs to those caused by ABZ or MTZ. Light microscopy revealed that changes in PSCs exposed to MTZ derivatives and ABZ reflected their relative actions in targeting scolex hooks, suckers and tegument. MTZ-w and ABZ caused rupture of hooks, deformation in suckers and disintegration in tegument of both PSCs and in vitro cultured segmentation stage. Less detrimental changes occurred upon the exposure to other MTZ derivatives. Exposure of HC to MTZ-w and ABZ caused regression in their size, damage in germinal membrane, fragmentation of underlining tissue, and scaling of laminated membrane. MTZ-w warrants further assessment as a potential chemotherapeutic drug against cystic echinococcosis in both animals and humans.

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Novel route of infection in experimental model of hydatid disease

Cited by 2 publications

References 0 publications

Experiment of Hydatid Cyst in Two Strains (Camels and Goats) in Saudi Arabia

Experiment of Hydatid Cyst in Two Strains (Camels and Goats) in Saudi Arabia

In Vitro Activity of Novel Metronidazole Derivatives on Larval Stages of Echinococcus Granulosus

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