Novel Rhizobium lineages isolated from root nodules of the common bean (Phaseolus vulgaris L.) in Andean and Mesoamerican areas

Ribeiro, Renan Augusto; Ormeño‐Orrillo, Ernesto; Dall’Agnol, Rebeca Fuzinatto; Graham, Peter; Martı́nez-Romero, Esperanza; Hungría, Mariangela

doi:10.1016/j.resmic.2013.05.002

Cited by 68 publications

(61 citation statements)

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“…Similar results reported by Andrade, Murphy and Giller (2002) and Shamseldin and Werner (2005) as well as the predominant R. tropici, which nodulates P. vulgaris in southern Ecuador (Table 4). Although few studies have reported on the genetic diversity of Rhizobium in Ecuador (Ribeiro et al, 2013), this is the first report of species diversity in the southern region of the country. Similar to this study, R. etli, R. leguminosarum and R. tropici have been previously isolated from common bean nodules (Martínez-Romero, 2003;Aguilar;Riva;Peltzer, 2004;Oliveira et al, 2011) and are considered to be the predominant occupant species of bean nodules in the Andean region (Junier et al, 2014;Ribeiro et al, 2015).…”

Section: Resultsmentioning

confidence: 77%

“…However, the genetic diversity of rhizobianodulating beans in Ecuador is, for the most part, unknown (Ribeiro et al, 2015). In previous studies, Bernal and Graham (2001) and Ribeiro et al (2013) reported on the diazotrophic rhizobial communities in soils of northern and central Ecuador. Recently, a new species, Rhizobium ecuadorense sp.…”

Section: Resultsmentioning

confidence: 99%

“…nov., which was obtained from a common bean nodule, has been described as a lineage from Ecuador (Ribeiro et al, 2015). This suggests that Ecuador is the center of genetic diversification for the common bean (Debouck et al, 1993), and symbionts of the several species of Rhizobium should be isolated from this region (Ribeiro et al, 2013).…”

Section: Resultsmentioning

confidence: 99%

“…leguminosarum clade. One of these lineages, with representatives isolated mostly from Ecuador, seems to be a dominant lineage associated with beans from that northern and central region (Ribeiro et al, 2013). This was one of the first studies in this Andean region, and additional research must be conducted to increase our understanding of the distribution patterns of rhizobial species in Ecuador.…”

Section: Introductionmentioning

confidence: 92%

“…The few previous studies from the Ecuadorian Andean region have focused only on determining the phylogenetic potential of symbionts (Bernal;Graham, 2001;Ribeiro et al, 2013). However, the effect of rhizobia on plant phenotypic parameters and the variability among isolated strains are rarely studied under laboratory, greenhouse or field conditions.…”

Section: Introductionmentioning

confidence: 99%

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Genetic and phenotypic diversity of Rhizobium isolates from Southern Ecuador

et al. 2017

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Rhizobium-legume symbioses play relevant roles in agriculture but have not been well studied in Ecuador. The aim of this study was to characterize the genetic and phenotypic diversity of Rhizobium isolates associated with Phaseolus vulgaris from southern Ecuador. Morphocultural characterization, biochemical tests and physiological analyses were conducted to authenticate and determine the diversity of bacteria Rhizobium-like isolates. The genetic diversity of the isolates was determined by molecular techniques, which consisted of bacteria DNA extraction and amplification and sequencing of the 16S rRNA gene. The nodulation parameters and nitrogen fixation for P. vulgaris under greenhouse conditions were also assessed to determine the phenotypic diversity among isolates. Furthermore, bacteria indole-acetic-acid production was evaluated by the colorimetric method. Morpho-cultural and biochemical characteristic assessments demonstrated that Rhizobium-like bacteria was associated with the P. vulgaris nodules. The diversity among the isolates, as determined by physiological analyses, revealed the potential of several isolates to grow at different pH values, salinity conditions and temperatures. Partial sequencing of the 16S rRNA gene identified the Rhizobium genus in every sampling site. From a total of 20 aligned sequences, nine species of Rhizobium were identified. Nodule formation and biomass, as well as nitrogen fixation, showed an increase in plant phenotypic parameters, which could be influenced by IAA production, especially for the strains R. mesoamericanum NAM1 and R. leguminosarum bv. viciae COL6. These results demonstrated the efficiency of native symbiotic diazotrophic strains inoculants for legume production. This work can serve as the basis for additional studies of native Rhizobium strains and to help spread the use of biofertilizers in Ecuadorian fields.Index terms: Phaseolus vulgaris; diazotrophic bacteria; 16S rRNA gene; nodulation; indole acetic acid. RESUMOA simbiose Rhizobium-leguminosa desempenha um relevante papel na agricultura, entretanto não tem recebido suficiente atenção de estudos científicos no Equador. O objetivo deste artigo foi caracterizar a diversidade genética e fenotípica de isolados de Rhizobium associados com Phaseolus vulgaris do sul do Equador. A caracterização morfo-cultural, testes bioquímicos e análises fisiológicas foram realizados para autenticar e determinar a diversidade de isolados de bactérias Rhizobium. A diversidade genética foi determinada por por técnicas moleculares consistindo na extração de DNA genômico bacteriano, amplificação e sequenciamento parcial do gene 16SrRNA; e parâmetros de nodulação e fixação de nitrogênio de P. vulgaris sobre condições de estufa foram testados para determinar a diversidade fenotípica entre os isolados. Além disso, a produção de ácido indolacético foi avaliada por um método colorimétrico. A análise fisiológica da diversidade entre os isolados revelou o potencial de crescimento de diversos isolados em diferentes níveis de pH, sa...

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